| 1. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Carbone, R., et al.
(author)
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Pamela observation of the 2012 may 17 gle event
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
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Conference paper (peer-reviewed)abstract
- The PAMELA (Payload for Antimatter Matter Exploration and Light-nuclei Astrophysics) satellite-borne experiment has been collecting data in orbit since July 2006, providing accurate measurements of the energy spectra and composition of the cosmic radiation from a few hundred MeV/n up to hundred GeV/n. This wide interval of measured energies makes PAMELA a unique instrument for Solar Energetic Particle (SEP) observations. Not only does it span the energy range between the ground-based neutron monitor data and the observations of SEPs from space, but also PAMELA carries out the first direct measurements of the composition for the highest energy SEP events, including those causing Ground Level Enhancements (GLEs). PAMELA has registered many SEP events in solar cycle 24 including the 2012 May 17 GLE event (GLE 71), offering unique opportunities to address the question of high-energy SEP origin. Experimental performances and preliminary results on the 2012 May 17 events will be presented. We will discuss the derived particle injection time and compare with other time scales at the Sun including the flare and CME onset times.
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| 3. |
- Di Felice, V., et al.
(author)
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Solar modulation of galactic hydrogen and helium over the 23rd solar minimum with the PAMELA experiment
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
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Conference paper (peer-reviewed)abstract
- PAMELA has been orbiting the Earth for more than six years, gathering data on solar, galactic and trapped cosmic rays during the time of the last solar minimum. The apparatus comprises a time-of-flight system, a silicon-microstrip magnetic spectrometer, a silicon-tungsten electromagnetic calorimeter, an anticoincidence system, a shower tail catcher scintillator and a neutron detector. The combination of these devices allows charged particle and antiparticle identification over a wide energy range and with an unprecedented precision. The quasi-polar orbit of the instrument, with an inclination of 70 degrees, makes it possible to measure low energy particles starting from about 100 MeV/n. In this work we present the time and rigidity dependence of the galactic proton and helium fluxes over the first 4 years of operation during the A < 0 solar minimum of solar cycle 23.
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| 4. |
- Mocchiutti, E., et al.
(author)
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Cosmic–ray positron energy spectrum measured by PAMELA
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
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Conference paper (peer-reviewed)abstract
- The PAMELA satellite borne experiment is designed to study cosmic rays with great accuracy in a wide energy range. One of PAMELA’s main goal is the study of the antimatter component of cosmic rays. The experiment, housed on board the Russian satellite Resurs–DK1, was launched on June 15th 2006 and it is still taking data. In this work we present the measurement of galactic positron energy spectrum in the energy range between 500 MeV and few hundred GeV.
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| 5. |
- Ricci, M., et al.
(author)
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Study on 2012 march 7 solar particle event and forbush decrease with the PAMELA experiment
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
-
Conference paper (peer-reviewed)abstract
- The PAMELA (Payload for Antimatter Matter Exploration and Light-nuclei Astro-physics) space-borne experiment was launched on 15 June 2006 and has been continuously collecting data since then. The apparatus measures electrons, positrons, protons, anti-protons and heavier nuclei from about 100 MeV to several hundreds of GeV. The on-board instrumentation is built around a permanent magnet with a silicon microstrip tracker, providing charge and track detection information. During solar maximum conditions of solar cycle 24, PAMELA has been providing key information about solar energetic particles (SEPs) and their influence at Earth. We discuss here the recent 2012 March 7 SEP event with a brief comment on the subsequent Forbush decrease, registered by PAMELA. This event was also observed by Fermi/LAT exhibiting unprecedented time-extended γ-ray emission (> 100 MeV) lasting nearly 20 hours. We compare the derived accelerated ion population at the Sun with the ion population measured in space by PAMELA and discuss the implications for particle acceleration.
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| 6. |
- Formato, V., et al.
(author)
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Hydrogen and helium isotopes flux in cosmic rays with the PAMELA experiment
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
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Conference paper (peer-reviewed)abstract
- PAMELA is a satellite borne experiment designed to study with great accuracy cosmic rays of galactic, solar, and trapped nature, with particular focus on the antimatter component. The detector consists of a permanent magnet spectrometer core to provide rigidity and charge sign information, a Time-of-Flight system for velocity and charge information, a Silicon-Tungsten calorimeter and a Neutron detector for lepton/hadron identification. The beta and rigidity information allow to identify isotopes for Z = 1 and Z = 2 particles in the energy range 100 MeV/n to 1 GeV/n. In this work we will present the final PAMELA results on the H and He isotope fluxes measured during the 23rd solar minimum from 2006 to 2007. Such fluxes carry relevant information helpful in constraining parameters in galactic cosmic rays propagation models complementary to those obtained from other secondary to primary measurements such as the boron-to-carbon ratio.
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| 7. |
- Adriani, O., et al.
(author)
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Antiprotons in primary cosmic radiation with PAMELA experiment
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
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Conference paper (peer-reviewed)abstract
- The latest measurements of antiprotons spectrum and antiproton-to-proton ratio in primary cosmic rays with PAMELA experiment are presented. They are in good agreement with model of secondary production of antiprotons in Galaxy, but they do not completely rule other sources at the high-energies.
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| 8. |
- Adriani, O., et al.
(author)
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Time Dependence Of The Proton Flux Measured By Pamela During The 2006 July-2009 December Solar Minimum
- 2013
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In: Astrophysical Journal. - : IOP Publishing. - 0004-637X .- 1538-4357. ; 765:2, s. 91-
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Journal article (peer-reviewed)abstract
- The energy spectra of galactic cosmic rays carry fundamental information regarding their origin and propagation. These spectra, when measured near Earth, are significantly affected by the solar magnetic field. A comprehensive description of the cosmic radiation must therefore include the transport and modulation of cosmic rays inside the heliosphere. During the end of the last decade, the Sun underwent a peculiarly long quiet phase well suited to study modulation processes. In this paper we present proton spectra measured from 2006 July to 2009 December by PAMELA. The large collected statistics of protons allowed the time variation to be followed on a nearly monthly basis down to 400 MV. Data are compared with a state-of-the-art three-dimensional model of solar modulation.
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| 9. |
- Formato, V., et al.
(author)
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Galactic boron and carbon fluxes measured by the PAMELA experiment
- 2013
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In: Proceedings of the 33rd International Cosmic Rays Conference, ICRC 2013. - : Sociedade Brasileira de Fisica. - 9788589064293
-
Conference paper (peer-reviewed)abstract
- The PAMELA experiment is a satellite-borne apparatus that performs measurements of the cosmic radiation with a particular focus on antiparticles and light nuclei. The heart of experiment is a magnetic spectrometer to measure the particle rigidity and sign of charge. A Time-of-Flight system, a Silicon-Tungsten calorimeter, and a neutron detector allow particle identification and lepton/hadron discrimination. The apparatus is surrounded by a set of anticoincidence scintillation counters to reject multi-particle events. In this work we will present the Boron and Carbon fluxes measured by PAMELA from July 2006 to March 2008. Such data, and in particular the B/C flux ratio, can help the modelling of the galactic cosmic rays propagation. This can be a crucial point in predicting the astrophysical background of antimatter (positrons and antiprotons) in cosmic rays in the search for a dark matter signal.
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| 10. |
- Martucci, M., et al.
(author)
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Analysis on H spectral shape during the early 2012 SEPs with the PAMELA experiment
- 2014
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In: Nuclear Instruments and Methods in Physics Research Section A. - : Elsevier BV. - 0168-9002 .- 1872-9576. ; 742, s. 158-161
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Journal article (peer-reviewed)abstract
- The satellite-borne PAMELA experiment has been continuously collecting data since 2006. This apparatus is designed to study charged particles in the cosmic radiation. The combination of a permanent magnet, a silicon strip tracker and a silicon-tungsten imaging calorimeter, and the redundancy of instrumentation allow very precise studies on the physics of cosmic rays in a wide energy range and with high statistics. This makes PAMELA a very suitable instrument for Solar Energetic Particle (SEP) observations. Not only does it span the energy range between the ground-based neutron monitor data and the observations of SEPs from space, but PAMELA also carries out the first direct measurements of the composition for the highest energy SEP events, including those causing Ground Level Enhancements (GLEs). In particular, PAMELA has registered many SEP events during solar cycle 24, offering unique opportunities to address the question of high-energy SEP origin. A preliminary analysis on proton spectra behaviour during this event is presented in this work.
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