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| 2. |
- Hansson, Markus, et al.
(author)
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Targeting proteins to secretory lysosomes of natural killer cells as a principle for immunoregulation.
- 2003
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In: Molecular Immunology. - 1872-9142. ; 40:6, s. 363-372
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Journal article (peer-reviewed)abstract
- Secretory lysosomes of natural killer (NK) cells combine storage, regulated secretion and lysosomal activity. We asked whether one could target exogenous proteins to the secretory lysosomes of NK-cells for final delivery into a tumor site upon degranulation. cDNAs for both soluble and transmembrane (tm) proteins were expressed in the human YT-Indy NK-cell line. Targeting of a soluble TNF receptor (sTNFR1) was achieved by expressing a cDNA construct with a transmembrane sequence to facilitate ER-export and by incorporating a cytosolic sorting signal (Y) from CD63 to overcome constitutive secretion. The resulting sTNFR1-tm-Y was targeted to secretory lysosomes as confirmed by results from biosynthetic radiolabeling in combination with subcellular fractionation, immunoelectron microscopy, and immunofluorescence microscopy. A soluble sTNFR1 form was generated in the secretory lysosome by endogenous proteolytic activity. Expression of exogenous normally secretory non-membrane proteins, such as alpha1-microglobulin (alpha1-m) and alpha1-antitrypsin (alpha1-at) resulted mostly in constitutive secretion although a small amount of alpha1-microglobulin was targeted to secretory lysosomes. Our results suggest a potential for delivery of pharmacologically active agents into tumor sites by use of the NK-cell secretory lysosome as a carrier. (C) 2003 Elsevier Ltd. All rights reserved.
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| 3. |
- Maurin, Anna-Sofia, et al.
(author)
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Realistic metaphysics: An interview with D. H. Mellor
- 2001
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In: Theoria: a Swedish Journal of Philosophy. - 0040-5825. ; 67:2, s. 96-113
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Journal article (peer-reviewed)abstract
- THERE WAS A TIME when many philosophers agreed that metaphysics was dead. Anyone aquatinted with the works of D.H. Mellor knows that the subject is alive and well. Two young philosophers who are familiar with his work, Anna-Sofia Maurin and Johannes Persson, met him in Cambrige for an interview.
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| 4. |
- Persson, Katarina Sofia, 1972-
(author)
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Deformation zones in models and nature
- 2002
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Doctoral thesis (other academic/artistic)abstract
- Field studies encounter several complicating factors not studied in the models. Examples are oblique convergence, heterogeneous materials and thermal softening by intruding magmas. Within the deeply eroded Svecokarelian orogeny, studies in eastern Bergslagen indicate strain accommodation of the N-S orogenic shortening by regional E-W folding and shear along the conjugate Singö Shear Zone and Ornö Banded Series. Rising temperature resulted in migmatites affecting the strain accommodation resulting in decoupling and rotation of folds along one of the deformation zones.Deformation zones developed in convergent orogens have been studied in both analogue models and in nature. These studies have focused on a number of important factors controlling strain accommodation during orogenesis. The models show that the shape of the leading edge of the indenting continent controls whether the initial suture remains active or if an effective indenter develops, the spacing and number of faults, the width of the orogen and the height of the mountains. All these characteristics depend on the rate and spatial distribution of erosion and sedimentation. Erosion decreases the importance of effective indenters and favors shearing on existing faults leading to steeper, longer lived shears bounding narrow orogens. If sediments load the margin (e.g. foreland), the thrusts propagate further outboard widening the orogen. The strain that is accommodated by compaction and shearing along deep décollement and conjugate imbricate shears is episodic in time. This work links episodes of increased rates of erosion and sedimentation to episodes of high uplift rates, i.e. the development of new imbricate thrusts or pop-up wedges.
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| 5. |
- Persson, Sofia, 1972
(author)
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Läraryrkets uppkomst
- 2004
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In: Presentation vid konferensen Lärares professionella liv, Öland, augusti 2004..
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Conference paper (other academic/artistic)
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| 7. |
- Rondin Lindberg, Sofia, et al.
(author)
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Interactions between the leukaemia-associated ETO homologues of nuclear repressor proteins.
- 2003
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In: European Journal of Haematology. - : Wiley. - 1600-0609 .- 0902-4441. ; 71:6, s. 439-447
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Journal article (peer-reviewed)abstract
- The eight-twenty-one (ETO) homologues, represented by ETO, myeloid transforming gene-related protein 1 (MTGR1) and myeloid transforming gene chromosome 16 (MTG16), are nuclear repressor proteins. ETO is part of the fusion protein acute myeloid leukaemia (AML)1-ETO, resulting from the translocation (8;21). Similarly, MTG16 is disrupted to become part of AML1/MTG16 in t(16;21). The aberrant expression of these chimeras could affect interplay between ETO homologues and contribute to the leukaemogenic process. We investigated possible interactions between the ETO homologues. Ectopic co-expression in COS-cells resulted in heterodimerisation of the various ETO homologues suggesting that they may co-operate. Similarly, the chimeric oncoprotein AML1-ETO interacted with both MTGR1 and MTG16. However, results from cell lines endogenously expressing more than one ETO homologue did not demonstrate co-precipitation. Results from IP-Western and size determination by gel filtration of deletion mutants expressed in COS-cells, indicated an important role of the HHR domain for oligomerisation. A role was also suggested for the Nervy domain in the homologue interactions. Our results suggest that ETO homologues can interact with each other as well as with AML1-ETO, although it is unclear as to what extent these interactions occur in vivo.
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