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- Mattsson, K, et al.
(author)
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Latent nuclear antigen of Kaposi's sarcoma herpesvirus/human herpesvirus-8 induces and relocates RING3 to nuclear heterochromatin regions
- 2002
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In: The Journal of general virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 83:Pt 1, s. 179-188
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Journal article (peer-reviewed)abstract
- LANA, the major latency-associated nuclear antigen of Kaposi’s sarcoma herpesvirus/human herpesvirus-8 (KSHV/HHV-8), binds RING3 protein, one of five human homologues of thefsh(female sterile homeotic) gene product ofDrosophila. In KSHV/HHV-8-infected cells LANA and the viral episomes accumulate in heterochromatin-associated nuclear bodies. Here we show that in several KSHV/HHV-8-negative cell lines derived from carcinomas, sarcomas and lymphomas, RING3 was expressed at low levels, primarily localized to the euchromatin, and dissociated from the chromosomes during mitosis. In contrast, in KSHV/HHV-8-infected body cavity lymphoma cells the bulk of RING3 localizes to the LANA nuclear bodies and remains associated with the chromosomes during cell division. KSHV/HHV-8-infected body cavity lymphoma cells expressed RING3 at much higher levels than cells without the virus. Transfection of full-length LANA, but not the C terminus alone, greatly induced RING3 gene expression, and LANA and RING3 co-localized even in the transfected cells, in the absence of KSHV/HHV-8 viral DNA. High levels of LANA expression led to the disappearance of heterochromatin in both human and mouse cells. We suggest that LANA and RING3 may create a local euchromatic microenvironment around the viral episomes that are anchored to the heterochromatin.
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| 2. |
- Yong, Yu, 1972, et al.
(author)
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Observation of Carbon Disulfide by Differential Optical Absorption Spectroscopy in Shanghai
- 2004
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In: Geophysical Research Letters. - 1944-8007 .- 0094-8276. ; 109:11, s. L11107-
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Journal article (peer-reviewed)abstract
- Carbon disulfide, CS 2 , is an important sulfur-containing species in the global environment. Its oxidation to SO 2 and COS are basic atmospheric chemical processes in the sulfur cycle. The typical measurement methods for CS 2 are gas chromatography with flame photometric detection (GC-FPD) and gas chromatography - mass spectrometry (GC-MS). This paper presents CS 2 and SO 2 long-term measurements made by the long path Differential Optical Absorption Spectroscopy (DOAS) technique in the winter of 2001 to 2002 in the urban area of Shanghai, China. We found a good correlation between SO 2 and CS 2 with average [SO 2 ]/[CS 2 ] ≈ 2.1. Combined with an analysis of pollution sources near the measurement site, our measurements indicate that CS 2 came mainly from a coal burning furnace close to the measurement site. Copyright 2004 by the American Geophysical Union.
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