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Träfflista för sökning "WFRF:(Ramakrishnan P) srt2:(2005-2009)"

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1.	Osmanov, S, et al. (author) Executive summary and recommendations from the WHO/UNAIDS/IAVI expert group consultation on 'Phase IIB-TOC trials as a novel strategy for evaluation of preventive HIV vaccines', 31 January-2 February 2006, IAVI, New York, USA 2007 In: AIDS (London, England). - 0269-9370. ; 21:4, s. 539-546 Journal article (peer-reviewed)
2.	Krengel, Ute, 1964, et al. (author) Preliminary X-ray crystallographic analysis of the secreted chorismate mutase from Mycobacterium tuberculosis: A tricky crystallization problem solved 2006 In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. - 1744-3091. ; 62:5, s. 441-445 Journal article (peer-reviewed)abstract Chorismate mutase catalyzes the conversion of chorismate to prephenate in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and plants. Here, the crystallization of the unusual secreted chorismate mutase from Mycobacterium tuberculosis (encoded by Rv1885c), a 37.2 kDa dimeric protein belonging to the AroQγ subclass of mutases, is reported. Crystal optimization was non-trivial and is discussed in detail. To obtain crystals of sufficient quality, it was critical to initiate crystallization at higher precipitant concentration and then transfer the drops to lower precipitant concentrations within 5-15 min, in an adaptation of a previously described technique [Saridakis & Chayen (2000), Protein Sci. 9, 755-757]. As a result of the optimization, diffraction improved from 3.5 to 1.3 Å resolution. The crystals belong to space group P21, with unit-cell parameters a = 42.6, b = 72.6, c = 62.0 Å., β = 104.5°. The asymmetric unit contains one biological dimer, with 167 amino acids per protomer. A soak with a transition-state analogue is also described.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Warren, M. (1); Schmidt, C. (1); Fast, PE (1); Kaleebu, P (1); Smith, P. (1); Gray, R (1); show more...; Sandstrom, E (1); Cooper, D (1); Foulkes, M (1); Dey, Raja, 1963 (1); Fix, A. (1); Allen, S (1); Levin, J (1); Peters, B (1); Krengel, Ute, 1964 (1); Churchyard, G (1); Johnston, M (1); Rees, H (1); Follmann, D (1); Karita, E (1); Birx, D (1); Ökvist, M. (1); Pitisuttithum, P (1); Sasso, S. (1); Ramakrishnan, C. (1); Kast, P. (1); Osmanov, S (1); Ackland, J (1); Barth-Jones, D (1); Chomba, E (1); Duerr, A (1); Jin, SG (1); Hutubessy, R (1); Malone, S (1); Indrayan, A (1); Mathieson, BJ (1); Mastro, TD (1); McNeil, J (1); Robertson, MN (1); Ramakrishnan, R (1); Rida, W (1); Ruan, YH (1); Self, S (1); Thiry, G (1); Wasserheit, J (1); Priddy, F (1); Thior, I (1); Macklin, R (1); Tangwa, G (1); show less...

University: Chalmers University of Technology (1); Karolinska Institutet (1)

Language: English (2)

Research subject (UKÄ/SCB): Natural sciences (1)

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