| 1. |
|
|
| 2. |
- Bezivin, J., et al.
(author)
-
OCL and model driven engineering
- 2005
-
In: Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). - 1611-3349 .- 0302-9743. ; 3297, s. 67-75
-
Journal article (peer-reviewed)abstract
- Precise modeling is essential to the success of the OMG's Model Driven Architecture initiative. At the modeling level (M1) OCL allows for the precision needed to write executable models. Can OCL be extended to become a full high-level executable language with side-effects? At the meta-level (M2), queries, views and transformations are subjects that will be vital to the success of the OMG's Model Driven Architecture initiative. Will OCL 2.0 become an essential part of the Queries/Views/Transformations standard and what will be its application areas in industry? Can the features of OCL 2.0 be used in the Model Driven Engineering (MDE) approach? This workshop aims at bringing together people from academia that are expected to report on inspiring ideas for innovative application scenarios and tools, and industrial practitioners, which are expected to provide statements on their view of the future of OCL in the context of MDE.
|
|
| 3. |
|
|
| 4. |
- Franzen, Oscar, et al.
(author)
-
Draft genome sequencing of Giardia intestinalis assemblage B isolate GS : is human giardiasis caused by two different species?
- 2009
-
In: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7366 .- 1553-7374. ; 5:8, s. e1000560-
-
Journal article (peer-reviewed)abstract
- Giardia intestinalis is a major cause of diarrheal disease worldwide and two major Giardia genotypes, assemblages A and B, infect humans. The genome of assemblage A parasite WB was recently sequenced, and the structurally compact 11.7 Mbp genome contains simplified basic cellular machineries and metabolism. We here performed 454 sequencing to 16 x coverage of the assemblage B isolate GS, the only Giardia isolate successfully used to experimentally infect animals and humans. The two genomes show 77% nucleotide and 78% amino-acid identity in protein coding regions. Comparative analysis identified 28 unique GS and 3 unique WB protein coding genes, and the variable surface protein (VSP) repertoires of the two isolates are completely different. The promoters of several enzymes involved in the synthesis of the cyst-wall lack binding sites for encystation-specific transcription factors in GS. Several synteny-breaks were detected and verified. The tetraploid GS genome shows higher levels of overall allelic sequence polymorphism (0.5 versus <0.01% in WB). The genomic differences between WB and GS may explain some of the observed biological and clinical differences between the two isolates, and it suggests that assemblage A and B Giardia can be two different species.
|
|
| 5. |
- Johansson, Olof, et al.
(author)
-
Bistable Molecular Switches Based on Linkage Isomerization in Ruthenium Polypyridyl Complexes with a Ligand-Bound Ambidentate Motif
- 2009
-
In: Chemistry - A European Journal. - : Wiley. - 0947-6539 .- 1521-3765. ; 15:5, s. 1195-1204
-
Journal article (peer-reviewed)abstract
- Electron-transfer-induced linkage isomerization was investigated in a series of bis-tridentate Ru polypyridyl complexes [Ru(L-X-OH)(Y-tpy)](2+) with ambidentate ligand L-X-OH bpy-C(R)(OH)-py (bpy-2,2'-bipyridine py-pyridine; R-H, Me, Ph, or tBu) and spectator ligand Y-tpy (tpy 2.2':6',2 ''-terpyridine, Y-p-tolyl, p-PhCO2Me, Cl, OEt, N-pyrrolidine). The ligand-bound ambidentate motif switches reversibly between N and O coordination in the Ru-II and Ru-III state,respectively. The potentials of the Ru-III/II couple differ by about 0.5 V between the isomers, and this results in a bistable electrochemical response of the molecular switches. The effects of structural modifications in form of substituents on the linking carbon atom of the ambidentate ligand and on the central pyridine moiety of the spectator ligand were investigated by electrochemical and computational methods. Differences in isomerization behavior span six orders of magnitude in rate constants and two orders of magnitude in equilibrium constants. The results can be interpreted in terms of steric and electronic substituent effects and their influence on rotational barriers, ligation geometry, and electron deficiency of the metal center.
|
|
| 6. |
- Reiner, David S., et al.
(author)
-
Synchronisation of Giardia lamblia : identification of cell cycle stage-specific genes and a differentiation restriction point
- 2008
-
In: International Journal of Parasitology. - : Elsevier BV. - 0020-7519 .- 1879-0135. ; 38:8-9, s. 935-944
-
Journal article (peer-reviewed)abstract
- The intestinal parasite Giardia lamblia undergoes cell differentiations that entail entry into and departure from the replicative cell cycle. The pathophysiology of giardiasis depends directly upon the ability of the trophozoite form to replicate in the host upper small intestine. Thus, cell proliferation is tightly linked to disease. However, studies of cell cycle regulation in Giardia have been hampered by the inability to synchronise cultures. Here we report that Giardia isolates of the major human genotypes A and B can be synchronised using aphidicolin, a mycotoxin that reversibly inhibits replicative DNA polymerases in eukaryotic cells. Aphidicolin arrests Giardia trophozoites in the early DNA synthesis (S) phase of the cell cycle. We identified a set of cell cycle orthologues in the Giardia genome using bioinformatic analyses and showed that synchronised parasites express these genes in a cell cycle stage-specific manner. The synchronisation method also showed that during encystation, exit from the ordinary cell cycle occurs preferentially in GZ and defines a restriction point for differentiation. Synchronisation opens up possibilities for further molecular and cell biological studies of chromosome replication, mitosis and segregation of the complex cytoskeleton in Giardia.
|
|
