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Träfflista för sökning "WFRF:(Sibirny Andriy A) srt2:(2016)"

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1.	Klionsky, Daniel J, et al. (author) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). 2016 In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 12:1, s. 1-222 Journal article (peer-reviewed)
2.	Schifferdecker, Anna, et al. (author) Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast. 2016 In: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 1432-0614 .- 0175-7598. Journal article (peer-reviewed)abstract Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Zhang, Yan (1); Korhonen, Laura (1); Lindholm, Dan (1); Vertessy, Beata G. (1); Wang, Mei (1); Wang, Xin (1); show more...; Liu, Yang (1); Kumar, Rakesh (1); Wang, Dong (1); Li, Ke (1); Liu, Ke (1); Zhang, Yang (1); Nàgy, Péter (1); Kominami, Eiki (1); van der Goot, F. Gis ... (1); Bonaldo, Paolo (1); Thum, Thomas (1); Adams, Christopher M (1); Minucci, Saverio (1); Vellenga, Edo (1); Swärd, Karl (1); Nilsson, Per (1); De Milito, Angelo (1); Zhang, Jian (1); Shukla, Deepak (1); Kågedal, Katarina (1); Chen, Guoqiang (1); Liu, Wei (1); Cheetham, Michael E. (1); Sigurdson, Christina ... (1); Clarke, Robert (1); Zhang, Fan (1); Gonzalez-Alegre, Ped ... (1); Jin, Lei (1); Chen, Qi (1); Taylor, Mark J. (1); Romani, Luigina (1); Wang, Ying (1); Kumar, Ashok (1); Simons, Matias (1); Ishchuk, Olena (1); Piskur, Jure (1); Ishaq, Mohammad (1); Yang, Qian (1); Algül, Hana (1); Brest, Patrick (1); Simon, Hans-Uwe (1); Mograbi, Baharia (1); Florey, Oliver (1); Ström, Anna-Lena (1); show less...

University: Lund University (2); Umeå University (1); Stockholm University (1); Linköping University (1); Karolinska Institutet (1); Swedish University of Agricultural Sciences (1)

Language: English (2)

Research subject (UKÄ/SCB): Natural sciences (2); Medical and Health Sciences (1)

Year: 2016 (2)Undo refine

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