| 1. |
- Aboualizadeh, F., et al.
(author)
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Mapping the Phospho-dependent ALK Interactome to Identify Novel Components in ALK Signaling
- 2021
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In: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836. ; 433:23
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Journal article (peer-reviewed)abstract
- Protein-protein interactions (PPIs) play essential roles in Anaplastic Lymphoma Kinase (ALK) signaling. Systematic characterization of ALK interactors helps elucidate novel ALK signaling mechanisms and may aid in the identification of novel therapeutics targeting related diseases. In this study, we used the Mammalian Membrane Two-Hybrid (MaMTH) system to map the phospho-dependent ALK interactome. By screening a library of 86 SH2 domain-containing full length proteins, 30 novel ALK interactors were identified. Many of their interactions are correlated to ALK phosphorylation activity: oncogenic ALK mutations potentiate the interactions and ALK inhibitors attenuate the interactions. Among the novel interactors, NCK2 was further verified in neuroblastoma cells using co-immunoprecipitation. Modulation of ALK activity by addition of inhibitors lead to concomitant changes in the tyrosine phosphorylation status of NCK2 in neuroblastoma cells, strongly supporting the functionality of the ALK/NCK2 interaction. Our study provides a resource list of potential novel ALK signaling components for further study. (C) 2021 The Authors. Published by Elsevier Ltd.
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| 3. |
- Beer, Sven, et al.
(author)
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Basic Concepts and Key Parameters of Chlorophyll Fluorescence
- 2021
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In: Research Methods of Environmental Physiology in Aquatic Sciences. - Singapore : Springer. - 9789811553530 - 9789811553547 ; , s. 221-229
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Book chapter (other academic/artistic)abstract
- Photosynthetic rates can be measured in several different ways. Classically, phytoplankton productivity has been measured by uptake rates of 14C-labelled inorganic carbon (Ci, i.e. CO2 and HCO3−), which is a sensitive measure even for sparse phytoplankton assemblages. For macrophytes, gas exchange of CO2 or O2 have been used as preferred methods of photosynthetic productivity. All these above mentioned methods have the disadvantage of being slow (minutes to hours or days) and the need for enclosing the plants, the latter of which invariably alters their surroundings from natural ones in terms of light (both irradiance and spectrum), water flow and decreasing nutrient, such as Ci, levels, as well as increasing O2 concentrations, which may impede on rates. For the last 20 years, chlorophyll fluorescence measurements have increasingly replaced the other methods as being both sensitive and speedy, and applicable under natural, non-enclosed, conditions. This chapter explains the background to chlorophyll fluorescence, as well as the principles of pulse-amplitude modulated (PAM) fluorometry and, briefly, fast repetition rate fluorometry (FRRF) measurements.
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| 4. |
- Beer, Sven, et al.
(author)
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Carbon Dioxide vs. Bicarbonate Utilisation
- 2021
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In: Research Methods of Environmental Physiology in Aquatic Sciences. - Singapore : Springer. - 9789811553530 - 9789811553547 ; , s. 153-164
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Book chapter (other academic/artistic)abstract
- While CO2 is the external inorganic carbon (Ci) form used for photosynthesis in terrestrial plants, the higher concentration of bicarbonate (HCO3−) in most water bodies renders this ionic Ci form the preferred external Ci source for most cyanobacteria, microalgae, and submerged macrophytes. The equilibrium concentrations of these two Ci forms depend largely on pH. For example, at today’s average seawater pH of 8.1, the HCO3− concentration is approximately 120 times that of dissolved CO2; in most lakes and streams pH values are lower so this ratio is reduced in favour of CO2 (but this Ci form is seldom higher than that of HCO3−), and HCO3− utilisation becomes less advantageous. On the other hand, the affinity of aquatic phototrophs for CO2 is much higher than that for HCO3−, and the preferred Ci form will thus be a compromise between the affinities for, and concentrations of, the two Ci forms. This chapter will outline ways to determine whether, and to what degree, external CO2 or HCO3− is utilised by various aquatic phototrophs.
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| 5. |
- Chi, C. -H, et al.
(author)
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Message from the program co-chairs
- 2011
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In: 15th IEEE International EDOC Enterprise Computing Conference, EDOC 2011. - 1541-7719. ; , s. ix-x
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Journal article (peer-reviewed)
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| 6. |
- Dittmar, Jakob, 1971-
(author)
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Comics für Blinde und Sehbehinderte?
- 2022
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Conference paper (other academic/artistic)abstract
- Taktile Comics für blinde und sehbehinderte Leserinnen und Leser sind eine gut gemeinte Idee, die jedoch in der praktischen Umsetzung schnel an Grenzen stößt. Besonders für Geburtsblinde ist das Konzept des Comics schwierig, in dem die Unterschiede zwischen Abbildungen bedeutungs- und erzählungstragend sind: zwar können Bilder im relief oder auf andere tastbare Form mit den Händen lesbar werden, aber der Vergleich und der damit verbundene Leseaufwand sind enorm. Auch die räumlichen Konzepte, die dabei Bedeutung bekommen, müssen erst gelernt werden.Wohingegen für Blinde mit Vorerfahrung von Comics und Bilderbüchern (vor der Erblindung) taktile Comics Freude machen können, so scheint das Prizip Comic für die allermeisten Geburtsblinden höchstens als intellektuelle Herausforderung von Interesse, Lesen zur Unterhaltung jedoch geschieht anders für diese Zielgruppe. Grenzen der medialen Anwendbarkeit und Zugänglichkeit werden diskutiert, die Gefahren von Fehleinschätzungen bei medialen Entwicklungen und Anwendungen, samt deren Erforschung, werden am Beispiel vertieft.
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| 7. |
- Fitzgerald, John, 1979, et al.
(author)
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Evolution of the AWS Waveswing(TM) MkII Concept
- 2008
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In: 2nd International Conference on Ocean Energy (ICOE 2008), 15th – 17th October 2008, Brest, France. ; ICOE 2008, s. 9-
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Journal article (other academic/artistic)abstract
- The evolution of the Archimedes Waveswing™concept up to 2007 is described. AWS Ocean EnergyLtd have continued to tackle the fundamentalfunctional issues like absorption, control, powerconversion and power export as they apply to theWaveswing™, but now with a particular emphasis onthe equally essential lifecycle goals such asmaintainability, manufacturability, deployability andof course overall economic viability. These effortshave been centred on the evolving concept design of aMk II Waveswing™ demonstrator for deployment atthe EMEC test site. At its core, the Mk II is stillfundamentally the same wave power concept of avariable displacement submerged absorber with itsinherent broadbandedness and the ability to respondeasily to long period wave excitations. However, someimportant developments have been made since theMk I pilot in Portugal and this paper highlights somepractical examples of how the Waveswing™ concepthas matured since early testing.
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| 9. |
- Stern, Jenny, 1987-, et al.
(author)
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Quality of web-based information at the beginning of a global pandemic : a cross-sectional infodemiology study investigating preventive measures and self care methods of the coronavirus disease 2019
- 2021
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In: BMC Public Health. - : BioMed Central (BMC). - 1471-2458. ; 21
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Journal article (peer-reviewed)abstract
- Background: Reducing the spread and impact epidemics and pandemics requires that members of the general population change their behaviors according to the recommendations, restrictions and laws provided by leading authorities. When a new epidemic or pandemic emerges, people are faced with the challenge of sorting through a great volume of varied information. Therefore, the dissemination of high-quality web-based information is essential during this time period. The overarching aim was to investigate the quality of web-based information about preventive measures and self care methods at the beginning of the COVID-19 pandemic.Methods: In May 2020, consumer-oriented websites written in Swedish were identified via systematic searches in Google (n = 76). Websites were assessed with inductive content analysis, the JAMA benchmarks, the QUEST tool and the DISCERN instrument.Results: Seven categories and 33 subcategories were identified concerning preventive measures (md = 6.0 subcategories), with few specifying a method for washing hands (n = 4), when to sanitize the hands (n = 4), and a method for sanitizing the hands (n = 1). Eight categories and 30 subcategories were identified concerning self care methods (md = 3.0 subcategories), with few referring to the national number for telephone-based counseling (n = 20) and an online symptom assessment tool (n = 16). Overall, the median total quality scores were low (JAMA = 0/4, QUEST =13/28, DISCERN = 29/80).Conclusions: At the beginning of the pandemic, substantial quality deficits of websites about COVID-19 may have counteracted the public recommendations for preventive measures. This illustrates a critical need for standardized and systematic routines on how to achieve dissemination of high-quality web-based information when new epidemics and pandemics emerge.
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