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- Hillarp, Andreas, et al.
(author)
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The 20210 A allele of the prothrombin gene is a common risk factor among Swedish outpatients with verified deep venous thrombosis
- 1997
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In: Thrombosis and Haemostasis. - 0340-6245. ; 78:3, s. 2-990
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Journal article (peer-reviewed)abstract
- A dimorphism in the 3'-untranslated region of the prothrombin gene (G to A transition at position 20210) has recently been reported to be associated with increases in plasma prothrombin levels and in the risk of venous thrombosis. We have examined the prothrombin dimorphism among 99 unselected outpatients with phlebography verified deep venous thrombosis, and in 282 healthy controls. The prevalence of the 20210 A allele was 7.1% (7/99) in the patient group, and 1.8% (5/282) in the healthy control group (p = 0.0095). The relative risk of venous thrombosis was calculated to be 4.2 (95% CI, 1.3 to 13.6), and was still significant when adjustment was made for age, sex and the factor V:R506Q mutation causing APC resistance [odds ratio 3.8 (95% CI, 1.1 to 13.2)]. As previously reported, 28% of the patients were carriers of the factor V:R506Q mutation. Thus, 34% (one patient carried both traits) of unselected patients with deep venous thrombosis were carriers of an inherited prothrombotic disorder. To sum up, our results confirm the 20210 A allele of the prothrombin gene to be an important risk factor for venous thrombosis.
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- Sundstrom, Jens, et al.
(author)
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MADS-box genes active in developing pollen cones of Norway spruce (Picea abies) are homologous to the B-class floral homeotic genes in angiosperms
- 1999
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In: Developmental Genetics. - 0192-253X .- 1520-6408. ; 25:3, s. 253-266
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Journal article (peer-reviewed)abstract
- The reproductive organs of conifers, the pollen cones and seed cones, differ in morphology from the angiosperm flower in several fundamental respects. In this report we present evidence to suggest that the two plant groups, in spite of these morphological differences and the long evolutionary distance between them, share important features in regulating the development of the reproductive organs. We present the cloning of three genes, DAL11, DAL12, and DAL13, from Norway spruce, all of which are related to the angiosperm B-class of homeotic genes. The B-class genes determine the identities of petals and stamens. They are members of a family of MADS-box genes, which also includes C-class genes that act to determine the identity of carpels and, in concert with B genes specify stamens in the angiosperm flower. Phylogenetic analyses and the presence of B-class specific C-terminal motifs in the DAL protein sequences imply homology to the B-class genes. Specific expression of all three genes in developing pollen cones suggests that the genes are involved in one aspect of B function, the regulation of development of the pollen-bearing organs. The different temporal and spatial expression patterns of the three DAL genes in the developing pollen cones indicate that the genes have attained at least in part distinct functions. The DAL11, DAL12, and 13 expression patterns in the pollen cone partly overlap with that of the previously identified DAL2 gene, which is structurally and functionally related to the angiosperm C-class genes. This result supports the hypothesis that an interaction between B- and C-type genes is required for male organ development in conifers like in the angiosperms. Taken together, our data suggests that central components in the regulatory mechanisms for reproductive organ development are conserved between conifers and angiosperms and, thus, among all seed plants.
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- Svensson, Erik
(author)
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Pharmacodynamic effects of antibiotics on growing and nongrowing bacteria
- 1997
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Doctoral thesis (other academic/artistic)abstract
- The main objective of this thesis was to study antibiotic effects on nongrowing baCteria and to study the development of resistance in Staphylococcus epidermidis, a bacterium which is often found in biofilm infections. The antibiotic effect on biofilm infections is generally weak, which probably is due to decreased growth rate of the bacteria. The effects of antibiotics on growing and nongrowing bacteria were studied with pharmacodynamic parameters. One of these parameters is postantibiotic effect (PAE). The conventional PAE measurement is performed with viable count. Viable counting is very laborious, which is why other methods have been used. However, the results may differ from those obtained with the viable count method for determining PAE. In this study effective regrowth time (ERT) and control-related effective regrowth time (CERT) were shown to be independent of the method used for bacterial quantification. With CERT and PAE it possible to study antibiotic effects on nongrowing and growing bacteria.CERT and PAE of amikacin, ciprofloxacin, and imipenem were investigated on growing and nongrowing Escherichia coli and Pseudonumas aeruginosa. All drugs had long CERT and PAE on growing bacteria. Arnikacin and ciprofloxacin, but not imipenem had long CERT and PAE on nongrowing bacteria.Amikacin, imipenem, ofloxacin, rifampicin, and vancomycin induced CERT and PAE on growing S. epidermidis, and imipenem combination with amikacin or vancomycin had a synergistic CERT and PAE in these cultures. Only rifampicin or antibiotic combinations containing rifampicin were effective against nongrowing S. epidermidis. There was no synergistic CERT or PAE on nongrowing bacteria.Selection and regrowth of highly rifampicin resistant S. epidermidis, which was present at a frequency of 10·7 in the initial inoculum, were seen when the bacteria were in~ubated with rifampicin at a high inoculum. Rifampicin combined with high concentrations of amikacin or ofloxacin prevents selection and regrowth of rifampicin resistant bacteria.
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- Svensson, Jonas, et al.
(author)
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Image artifacts due to a time-varying contrast medium concentration in 3D contrast-enhanced MRA
- 1999
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In: Journal of Magnetic Resonance Imaging. - 1522-2586. ; 10:6, s. 919-928
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Journal article (peer-reviewed)abstract
- The purpose of this work was to study image effects due to time-varying contrast medium concentration in contrast-enhanced three dimensional (3D) magnetic resonance angiography (MRA) images. Two different simulation models (1D and 3D) and two different contrast medium variation schemes were used. Phantom measurements were also performed. Experiments were performed for several different bolus timings. Similar sequence and image object parameters were used in both simulations and measurements (TE/TR 2. 1/7.8 mses, flip angle 30 degrees, T1/T2 1200-80/150-40 msec, flow velocity 100 cm/sec). A small variation in bolus timing yielded large variations in the appearance of the image effects, especially if the center of k-space was sampled in the vicinity of rapid contrast medium concentration variation. For a typical bolus injection in a patient, a severe signal loss but only minor ringing and edge artifacts appeared if the bolus injection was poorly timed. Effects of pulsatile flow were minor. The 3D model proved to be a useful tool in these studies.
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