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Träfflista för sökning "WFRF:(Svitel Juraj) srt2:(2004)"

Search: WFRF:(Svitel Juraj) > (2004)

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  • Masarova, Jana, et al. (author)
  • Mannan-penicillin G acylase neoglycoproteins and their potential applications in biotechnology
  • 2004
  • In: Biotechnology and Applied Biochemistry. - 1470-8744. ; 39:3, s. 285-291
  • Journal article (peer-reviewed)abstract
    • Mannan-penicillin G acylase neoglycoproteins were prepared by the conjugation of Saccharomyces cerevisiae mannan with enzyme penicillin G acylase using the reductive amination method. Eight neoglycoproteins preparations were obtained after gel chromatography. The preparations contained from 42 to 67% (w/w) saccharides and their molar masses varied from 283 to over 1000 kDa. Significant biospecific interaction of separated fractions with the lectin concanavalin A was evaluated by the precipitation and sorption method (equilibrium constants) and further characterized using surface plasmon resonance to determine kinetic association and dissociation constants. K-D was determined over the range 10(-7) M. High-molar-mass preparations appeared to be more suitable for preparation of stable and active complexes with concanavalin A for prospective use as a penicillin G acylase biocatalyst in enzyme reactors. The enzyme stability of such complexes was significantly increased compared with the original neoglycoprotein. Lower-molar-mass preparations were more suitable for applications such as biocatalysts in bioanalytical devices.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Danielsson, Bengt (2)
Gemeiner, P. (2)
Svitel, Juraj (2)
Mendichi, R. (1)
Nahalkova, J (1)
Masarova, Jana (1)
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Mislovicova, D (1)
Pribulova, B (1)
Petrus, L (1)
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University
Lund University (2)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (2)
Year
2004 (2)Undo refine

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