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- Pamela, J., et al.
(author)
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Overview of results and possibilities for fast particle research on JET
- 2002
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In: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 42:8, s. 1014-1028
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Journal article (peer-reviewed)abstract
- The large physical size of the JET tokamak, its heating systems and diagnostics, and its capability to operate with full deuterium-tritium (D-T) plasmas, including high-power tritium neutral beam injection (NBI), give it unique possibilities in fast particle research in fusion plasmas. These have already been used to generate significant (2-3 MW level) power in fusion a-particles in the 1997 D-T campaign. Recent JET experiments have concentrated on two important scenarios of relevance to next-step tokamak devices: the ELMy H-mode plasmas and plasmas with strong internal transport barriers (ITBs). The achieved progress will help in preparation for a possible second D-T experiment on JET. Fast particle studies have also been carried out recently using ion cyclotron resonance heating (ICRH)-accelerated particles and external-excitation methods to study Alfven eigenmodes (AEs). Looking towards the future, the capability of JET will be enhanced by upgrades to the NBI system, ICRH system and various diagnostics. Results of the first JET D-T experiment (DTE1) form a basis on which to elaborate a second D-T experiment (DTE2) which could be proposed after these enhancements. The alpha-physics part of this programme would be divided between the investigation of alpha-particle confinement, heating and loss processes in the 'integrated scenarios' (where the discharge is as close as possible to an ITER-relevant scenario), and dedicated 'alpha-physics' experiments, with specially prepared plasmas. In ELMy H-mode plasmas the fusion performance could roach Q(=P-fusion/P-input) of similar to0.33 at the highest combined heating powers, corresponding to similar to 6x10(-4), allowing a test of the margins of TAE stability in quasi-steady-state conditions. The integrated-scenario fast particle programme could concentrate on the instabilities and heating in plasma regimes with strong steady-state ITBs, with expected Q values similar to0.58 and similar to2x10(-3), demonstrating the compatibility of these operating scenarios with alpha-effects. Excitation of TAEs by alpha-particles in the plasma core could also be studied in such integrated scenarios. An issue which will receive attention is the confinement of MeV energy ions in the centre of ITB plasmas with strongly reversed shear, where the low current density in the centre may lead to the alpha-particles entering loss orbits. In preparation for a D-T campaign, studies of triton burn-up in deuterium ITB plasmas will begin in the 2002 experimental campaigns. Special 'afterglow' experiments to measure TAEs after the termination of the (stabilizing) NBI have already been explored in JET deuterium ITB scenarios and would be planned for DTE2. It is intended to develop special versions of ITB plasmas with dominant ion heating which would maximize the sensitivity to degradation of alpha-heating effects.
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- Czene, S, et al.
(author)
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DNA fragmentation and morphological changes in apoptotic human lymphocytes
- 2002
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In: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 294:4, s. 872-878
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Journal article (peer-reviewed)abstract
- Cell suspensions enriched in cells at various stages of apoptosis were obtained by separation of irradiated human peripheral blood lymphocytes on density gradients at different post-irradiation times. The state of DNA fragmentation in the cells was determined by comet assay and pulsed field gel electrophoresis. The morphologically distinguishable features of apoptosis such as chromatin condensation and cell shrinkage correlated with discrete stages of DNA fragmentation. It was found that greater than or equal to50kbp fragmentation of DNA occurs already in cells of normal density whereas the subsequent DNA fragmentation onto fragments <50 kbp occurs in parallel with cell shrinkage and simultaneous increase in cell density. The observed stages of DNA fragmentation seem to be separated in time that could allow in case of abortive apoptosis formation of chromosomal aberrations.
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- Magnusson, Per, et al.
(author)
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Monoclonal antibodies against tissue-nonspecific alkaline phosphatase : Report of the ISOBM TD9 Workshop
- 2002
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In: Tumor Biology. - : Springer Science and Business Media LLC. - 1010-4283 .- 1423-0380. ; 23:4, s. 228-248
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Journal article (peer-reviewed)abstract
- Nineteen monoclonal antibodies (MAbs) against tissue-nonspecific (liver/bone/kidney) alkaline phosphatase (TNALP) were investigated in the ISOBM TD-9 Workshop. These MAbs were generated with antigens obtained from human bone tissue (n = 9), human osteosarcoma cell lines (SaOS-2 and TPX, n = 7) and human liver tissue (n = 3). The evaluation included the following antigen forms: (a) commercially available preparations of human bone ALP (BALP) and liver ALP (LALP), (b) human BALP isoforms, B/I, B1 and B2, and (c) soluble secreted epitope-tagged recombinant human TNALP (setTNALP) expressed in COS-1, osteosarcoma (SaOS-2) and hepatoma (Huh2) cell lines. In addition, 16 TNALP mutant cDNAs corresponding to a wide spectrum of reported hypophosphatasia mutations were used in an attempt to map specific immunoreactive epitopes on the surface of the TNALP molecule. The TD-9 MAbs were evaluated by immunoradiometric (IRMA) assays, cross-inhibition and different enzyme immunoassay designs. No indications of explicit tissue discriminatory immunoreactivities of the investigated MAbs against TNALP were found. However, certain IRMA combinations of MAbs increased the specificity of BALP measurements. All MAbs bound to the three BALP isoforms B/I, B1 and B2, but none of the investigated MAbs were specific for any of the isoforms. Significant differences were, however, found in immunoreactivity between these isoforms, with cross-reactivities ranging from 21 to 109% between the two major BALP isoforms B1 and B2. Desialylation with neuraminidase significantly increased the MAb affinity for the BALP isoforms B/I, B1 and B2, and also decreased the observed differences in cross-reactivity between these isoforms. We suggest, therefore, that the MAb affinity is dependent on the amount/number of terminal sialic acid residues located at the five putative N-glycosylation sites. Based on the overall results, we present a putative three-dimensional model of the TNALP molecule with positioning of the four major antigenic domains (designated A-D) of the investigated MAbs. The TNALP molecule is depicted as a homodimer, hence most, but not necessarily all, epitopes are displayed twice. The antigenic domains were positioned with the following assumptions: domain A was positioned close to the active site since most of these MAbs interfered with the catalytic activity. Interestingly, both MAbs included in the commercial BALP kits were grouped with domain A. Moreover, 4 of the 5 putative N-glycosylation sites (with terminal sialic acid residues) are located within, or with close proximity to, domain A. Domain B was localized at the top flexible loop (crown domain) of the TNALP molecule. Domain C was clearly defined by the IRMA assay combinations and by site-directed mutants of TNALP to be close to residue E281, which is located near the fourth metal binding site, likely to be occupied by a calcium ion. Domain D was positioned close to residues A115, A162 and E174, but this domain was also close to the GPI anchor site. In conclusion, none of the 19 investigated TD-9 MAbs were entirely specific for BALP or LALP, thus indicating that all MAbs bind mainly to epitopes on the common protein core of BALP and LALP and/or common glycosylated epitopes. However, some MAbs (either single or in combination with other MAbs) work sufficiently well to measure BALP when the assayed samples do not contain elevated levels of LALP. Copyright © 2002 S. Karger AG, Basel.
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- Testa, I, et al.
(author)
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Physically Inspired Models for the Synthesis of Stiff Strings with Dispersive Waveguides
- 2004
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In: EURASIP Journal on Advances in Signal Processing. - : Springer. - 1687-6172 .- 1687-6180. ; 2004:7, s. 964-977
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Journal article (peer-reviewed)abstract
- We review the derivation and design of digital waveguides from physical models of stiff systems, useful for the synthesis of sounds from strings, rods, and similar objects. A transform method approach is proposed to solve the classic fourth-order equations of stiff systems in order to reduce it to two second-order equations. By introducing scattering boundary matrices, the eigenfrequencies are determined and their n2 dependency is discussed for the clamped, hinged, and intermediate cases. On the basis of the frequency-domain physical model, the numerical discretization is carried out, showing how the insertion of an all-pass delay line generalizes the Karplus-Strong algorithm for the synthesis of ideally flexible vibrating strings. Knowing the physical parameters, the synthesis can proceed using the generalized structure. Another point of view is offered by Laguerre expansions and frequency warping, which are introduced in order to show that a stiff system can be treated as a nonstiff one, provided that the solutions are warped. A method to compute the all-pass chain coefficients and the optimum warping curves from sound samples is discussed. Once the optimum warping characteristic is found, the length of the dispersive delay line to be employed in the simulation is simply determined from the requirement of matching the desired fundamental frequency. The regularization of the dispersion curves by means of optimum unwarping is experimentally evaluated.
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