| 1. |
- Andréasson, Sven, et al.
(author)
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Psychosocial Treatment for Alcohol Dependence
- 2003
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In: Treating Alcohol and Drug Abuse : An Evidence Based Review - An Evidence Based Review. - : Wiley. - 352730682X - 9783527306824 - 9783527601462 ; , s. 43-188
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Book chapter (peer-reviewed)
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| 2. |
- Berglund, Mats, et al.
(author)
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Pharmacotherapy for alcohol dependence
- 2003
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In: Treatment of Alcohol and Drug Abuse. An Evidence-Based Review. - 9783527306824 - 352730682X ; , s. 4-4
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Book chapter (other academic/artistic)
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| 3. |
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| 5. |
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| 6. |
- Chabes, Andrei, et al.
(author)
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Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit
- 2000
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 97:6, s. 2474-2479
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Journal article (peer-reviewed)abstract
- Ribonucleotide reductase (RNR) catalyzes the de novo synthesis of deoxyribonucleotides. Eukaryotes have an alpha(2)beta(2) form of RNR consisting of two homodimeric subunits, proteins R1 (alpha(2)) and R2 (beta(2)). The R1 protein is the business end of the enzyme containing the active site and the binding sites for allosteric effectors. The R2 protein is a radical storage device containing an iron center-generated tyrosyl free radical. Previous work has identified an RNR protein in yeast, Rnr4p, which is homologous to other R2 proteins but lacks a number of conserved amino acid residues involved in iron binding. Using highly purified recombinant yeast RNR proteins, we demonstrate that the crucial role of Rnr4p (beta') is to fold correctly and stabilize the radical-storing Rnr2p by forming a stable 1:1 Rnr2p/Rnr4p complex. This complex sediments at 5.6 S as a betabeta' heterodimer in a sucrose gradient. In the presence of Rnr1p, both polypeptides of the Rnr2p/Rnr4p heterodimer cosediment at 9.7 S as expected for an alpha(2)betabeta' heterotetramer, where Rnr4p plays an important role in the interaction between the alpha(2) and the betabeta ' subunits. The specific activity of the Rnr2p complexed with Rnr4p is 2,250 nmol deoxycytidine 5'-diphosphate formed per min per mg, whereas the homodimer of Rnr2p shows no activity. This difference in activity may be a consequence of the different conformations of the inactive homodimeric Rnr2p and the active Rnr4p-bound form, as shown by CD spectroscopy. Taken together, our results show that the Rnr2p/Rnr4p heterodimer is the active form of the yeast RNR small subunit.
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| 7. |
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| 8. |
- Ismail, S, et al.
(author)
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Direct observation of the molten state of nanometer-sized particles with an atomic force microscope: A feasibility study
- 2002
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In: Journal of Nanoparticle Research. - 1572-896X. ; 4:4, s. 351-356
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Journal article (peer-reviewed)abstract
- An atomic force microscope (AFM) was used to directly examine the physical state of nanometer-sized particles. The critical diameter of indium particles, where evidence of melting at room temperature was observed, was 7.8 +/- 1.2 nm. This conclusion is based on a method relying on the manipulation of particles in ambient air and at constant temperature. This method involves a simple set-up that permits a combination of both manipulation and imaging of individual particles. To determine whether a particle is molten, three criteria are used: the merging of particles to form bigger spherical particles, a tip-induced shape change, and the formation of nanofibers. All three criteria have been checked using other particle materials. An attempt at 56degreesC revealed oxidation of the indium particles as the major problem for melting investigation. Manipulations under high-purity nitrogen atmosphere support the validity of the findings. The use of the AFM to determine whether a nanoparticle is molten is, however, complicated by the oxidation issue.
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| 9. |
- Kortegaard, Carl, et al.
(author)
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Observation of the molten state of nano-particles with an atomic force microscope
- 2002
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In: 7th International Conference on Nanometer-Scale Science and Technology and 21st European Conference on Surface Science.
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Conference paper (peer-reviewed)abstract
- An atomic force microscope was used to directly examine the physical state of nanometer-sized particles. The critical diameter of indium particles, where evidence of melting at room temperature was observed, was 7.8±1.2 nm. This conclusion is based on a method relying on the manipulation of particles in ambient air and at constant temperature. This method involves a simple set up that permits a combination of both manipulation and imaging of individual particles. To determine whether a particle is molten, three criteria are used: the merging of particles to form bigger spherical particles, a tip-induced shape change and the formation of nanofibres. All three criteria have been checked using other particle materials. The use of the atomic force microscope to determine whether a nanoparticle is molten, is however complicated by oxidation
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| 10. |
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