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- Fridén, Thomas, et al.
(author)
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Function after anterior cruciate ligament injuries. Influence of visual control and proprioception
- 1998
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In: Acta Orthopaedica Scandinavica. - : Medical Journals Sweden AB. - 0001-6470. ; 69:6, s. 590-594
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Journal article (peer-reviewed)abstract
- Information about limb positions and movements consists of input from visual, vestibular, cutaneous, muscular, tendinous and joint receptors, but the relative contribution from each type and location of receptors is not known. The aim of this study was: a) to measure the contribution from visual control on extremity function, as measured with a one-leg hop test in healthy persons, in patients with an asymptomatic ACL injury, after non-operative treatment and in patients with a stable knee after an ACL reconstruction, b) to investigate if there was any relation between proprioception from the extremity, as measured with the threshold for detecting passive motion of the knee, and the one-leg hop test with a gradual decrease in visual control. There was a decrease in hop-length when the subjects were deprived of visual control that was significant when the dominant eye or both eyes were blinded, both in the 2 patient groups and the reference population. The magnitude of the length reduction did not differ between the groups or between injured and healthy limbs. In all 4 threshold tests performed as a measure of peripheral proprioception, a stronger relation to hop-length was recorded for the blinded hop than with full visual control in the patients with nonoperated ACL injuries. The coefficients of correlation between hop-length and the proprioceptive recordings in the injured limb were of the same magnitude as on the healthy side.
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| 4. |
- Fridén, Thomas, et al.
(author)
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Proprioception after an acute knee ligament injury: a longitudinal study on 16 consecutive patients
- 1997
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In: Journal of Orthopaedic Research. - : Wiley. - 1554-527X .- 0736-0266. ; 15:5, s. 637-644
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Journal article (peer-reviewed)abstract
- A defect in proprioception has been found in selected patient groups that have an anterior cruciate ligament deficient knee at different times after the original injury. The time of development and the extent of such defects were studied longitudinally on 16 consecutive patients. During the first year after a primary knee injury, which included a complete rupture of the anterior cruciate ligament, we repeatedly performed three tests of proprioception: (a) one to determine the threshold for detecting a passive motion from starting positions of 20 and 40 degrees, (b) an active reproduction of a passive angular change, and (c) a visual estimation of a passive angular change. The injured limb was compared with the uninjured limb and with the limbs of an age-matched reference group of healthy subjects. The population did not have a normal distribution, and some patients had consistently extreme recordings in the threshold tests at the various times of testing. Significant differences were found between the groups at the starting position of 20 degrees, when the injured knee was compared with the uninjured knee, after 1 month (p = 0.05), and after 2 months (p = 0.03). There was a trend toward a higher threshold for detecting a passive motion when the injured side was compared with the knees of the reference group at 1 month (p = 0.06) but not later on. A similar pattern was found for the injured knee at the starting position of 40 degrees, but it was not significant. An impaired ability to detect a passive motion was registered for the nearly extended knee 1 and 2 months after a primary injury. In the active reproduction and visual estimation tests, no significant defects were found at any time during the first year in these consecutively studied patients.
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| 5. |
- Fridén, Thomas, et al.
(author)
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Proprioception in the nearly extended knee. Measurements of position and movement in healthy individuals and in symptomatic anterior cruciate ligament injured patients
- 1996
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In: Knee Surgery, Sports Traumatology, Arthroscopy. - 1433-7347. ; 4:4, s. 217-224
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Journal article (peer-reviewed)abstract
- Proprioception of the knee was measured in 19 healthy individuals to evaluate whether there were any differences between extension and flexion movements from two different starting positions. The threshold before detecting a passive movement, visual estimation on a protractor of a passive change in position (30 degrees angular change) and active reproduction of the same angular change were registered. The reference population was tested twice to study normal variation and reproducibility, followed by the evaluation of 20 patients with chronic, symptomatic and unilateral anterior cruciate ligament (ACL)-deficient knees. In the normal population no differences were found between the right and the left leg, men and women, or measurements made at the first and at the second test occasion. The thresholds from a starting position of 20 degrees were lower for extension than for flexion. When comparing the thresholds for extension between the 20 degrees and the 40 degrees starting position, lower values were found in the more extended position. The thresholds for flexion were lower from the 40 degrees starting position than from the 20 degrees starting position. The active reproduction of an angular change of 30 degrees was more accurate during flexion (30 degrees-60 degrees) than during extension (60 degrees-30 degrees). There were no differences in the reproduction tests or in thresholds from the 40 degrees starting position between the patients and the normal group, but the patients had higher thresholds from the 20 degrees starting position, in movements towards both extension 1.0 degree (range 0.5 degree-12.0 degrees) and flexion 1.5 degrees (range 0.5 degree-10.0 degrees) than the normal group 0.75 degree (range 0.5 degree-2.25 degrees) (P = 0.01) and 1.0 degree (range 0.5 degree-3.0 degrees) (P = 0.06), respectively. Thus, information of passive movements in the nearly extended knee position was more sensitive towards extension than towards flexion in threshold tests and the sensitivity improved closer to full extension, which implies a logical joint protective purpose. In this nearly extended knee position, which is the basis for most weight-bearing activities, patients with symptomatic ACL-deficient knees had an impaired awareness in detecting a passive movement. There were no differences in the more flexed position or in the reproduction tests between the patients and the normal group, and reproduction tests in the present form seem less appropriate to use in the evaluation of ACL injuries.
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- Fridén, Thomas, et al.
(author)
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Proprioceptive defects after an anterior cruciate ligament rupture -- the relation to associated anatomical lesions and subjective knee function
- 1999
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In: Knee Surgery, Sports Traumatology, Arthroscopy. - : Springer Science and Business Media LLC. - 1433-7347 .- 0942-2056. ; 7:4, s. 226-231
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Journal article (peer-reviewed)abstract
- A disturbed proprioception has been described in patients with an anterior cruciate ligament (ACL) deficient knee. The relation to demographic data and to different commonly associated anatomical lesions, as well as to subjective knee function, was prospectively studied in 16 consecutive patients after an acute knee ligament injury. All patients had a complete rupture of the ACL, but variable associated anatomical lesions. The threshold to detect a passive motion, as a measure of their proprioceptive ability, was registered repeatedly during the first year after injury. Four of the patients had consistently severe and persistent deficits at 1, 2 and 8 months. These four individuals had more chondral lesions and a lower subjective rating of their knee function than the remaining patients. In the whole group there were significant correlations between the recorded thresholds and associated chondral lesions, meniscal lesions and the subjective rating of knee function. We found no significant relation between age, gender, activity level, grade of mechanical laxity increase or a medial collateral ligament rupture, and the proprioceptive recordings. Thus, morphological lesions other than a rupture of the ACL seem to contribute to the proprioceptive deficits after a knee ligament injury, and the patients' ability to detect a passive motion showed a relation to subjective knee function from the time of injury onwards.
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- Karlsson, Jan, 1966-, et al.
(author)
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Intracellular carbonic anhydrase of Chlamydomonas reinhardtii
- 1995
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In: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 109:2, s. 533-539
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Journal article (peer-reviewed)abstract
- An intracellular carbonic anhydrase (CA; EC 4.2.1.1) was purified to homogeneity from a mutant strain of Chlamydomonas reinhardtii (CW 92) lacking a cell wall. Intact cells were washed to remove periplasmic CA and were lysed and fractionated into soluble and membrane fractions by sedimentation. All of the CA activity sedimented with the membrane fraction and was dissociated by treatment with a buffer containing 200 mM KCI. Solubilized proteins were fractionated by ammonium sulfate precipitation, anionic exchange chromatography, and hydrophobic interaction chromatography. The resulting fraction had a specific activity of 1260 Wilbur-Anderson units/mg protein and was inhibited by acetazolamide (50% inhibition concentration, 12 nM). Final purification was accomplished by the specific absorption of the enzyme to a Centricon-10 microconcentrator filter. A single, 29.5-kD polypeptide was eluted from the filter with sodium dodecyl sulfate-polyacrylamide gel electrophoresis sample buffer, and a 1.5 M ammonium sulfate eluate contained CA activity. In comparison with human CA isoenzyme II, the N-terminal and internal amino acid sequences from the 29.5-kD polypeptide were 40% identical with the N-terminal region and 67% identical with an internal conserved region. Based on this evidence, we postulate that the 29.5-kD polypeptide is an internal CA in C. reinhardtii and that the enzyme is closely related to the alpha-type CAs observed in animal species.
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| 8. |
- Lacy, Thomas E., et al.
(author)
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Effects of damage distribution on evolution
- 1997
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In: Applications of continuum damage mechanics to fatigue and fracture. - : ASTM International. - 0803124732 ; , s. 131-149
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Book chapter (peer-reviewed)abstract
- Recent micromechanically inspired phenomenological theories using internal state variable (ISV) representations of damage have been used to predict the thermomechanical behavior of microcracked solids. These models do not, in an explicit manner, account for distributions of microcracks in a representative volume element (RVE) and have been used success-fully only to determine the effective moduli of damaged solids. It has been demonstrated that while the distribution and interaction of damage entities within an RVE generally have a minor effect on the effective moduli, it has a significant effect on the evolution of damage and failure at the macroscale. Damage evolution rates, in general, cannot be described adequately by such theories because of their inability to account for interactions between damage entities in an arbitrary distribution. Key issues pertaining to the development of viable damage evolution equations using a continuum damage mechanics approach are addressed. In particular, limitations associated with the use of ISVs that can be expressed either in terms of macroscopically measurable quantities or through a spatial average of the geometric features of individual damage entities are discussed. Numerical simulations of evolving crack systems in two-dimensional perfectly brittle solids indicate that "effective stress" models may have difficulty in characterizing damage evolution in brittle microcracked solids when the damage consists of cracks of variable size or spatial distributions. An argument for implementing ISVs based on higher-order moments of the damage distribution within an RVE is presented.
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| 9. |
- Lacy, Thomas E., et al.
(author)
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Gradient concepts for evolution of damage
- 1999
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In: Mechanics of materials. - 0167-6636 .- 1872-7743. ; 31:12, s. 831-860
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Journal article (peer-reviewed)abstract
- While low-order measures of damage have sufficed to describe the stiffness of bodies with distributed voids or cracks, such as the void volume fraction or the crack density tensor of Vakulenko, A.A., Kachanov, M., 1971., addressing the growth of distributed defects demands a more comprehensive description of the details of defect configuration and size distribution. Moreover, interaction of defects over multiple length scales necessitates a methodology to sort out the change of internal structure associated with these scales. To extend the internal state variable approach to evolution, we introduce the notion of multiple scales at which first and second nearest-neighbor effects of nonlocal character are significant, similar to homogenization theory. Further, we introduce the concept of a cutoff radius for nonlocal action associated with a representative volume element (RVE), which exhibits statistical homogeneity of the evolution, and flux of damage gradients averaged over multiple subvolumes. In this way, we enable a local description at length scales below the RVE. The mean mesoscale gradient is introduced to reflect systematic differences in size distribution and position of damage entities in the evolution process. When such a RVE cannot be defined, the evolution is inherently statistically inhomogeneous at all scales of reasonable dimension, and the concept of macroscale gradients of internal variables is the only recourse besides micromechanics. Based on a series of finite element calculations involving evolution of 2D cracks in brittle elastica arranged in random periodic arrays, we examine the evolution of the mean mesoscale gradients and note some preliminary implications for the utility of such an approach.
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| 10. |
- Lacy, Thomas E., et al.
(author)
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On representation of damage evolution in continuum damage mechanics
- 1997
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In: International journal of damage mechanics. - : SAGE Publications. - 1056-7895 .- 1530-7921. ; 6:1, s. 62-95
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Journal article (peer-reviewed)abstract
- The effect of damage patterning on elastic moduli and damage evolution in ideal brittle cracked solids is examined. Key limitations associated with typical continuum damage mechanics approaches are addressed. Critical shortcomings arising from the use of spatially-averaged damage descriptors in the evaluation of effective moduli and thermodynamic forces are investigated using numerical simulations of evolving two-dimensional crack systems. Fundamental elements of a higher-order continuum description of damage based on distribution functions are discussed, which directly include damage interaction effects.
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