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Träfflista för sökning "WFRF:(Torres Gabriela V.) srt2:(2022)"

Search: WFRF:(Torres Gabriela V.) > (2022)

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  • Chirikian, Orlando, et al. (author)
  • The effects of xeno-free cryopreservation on the contractile properties of human iPSC derived cardiomyocytes
  • 2022
  • In: Journal of Molecular and Cellular Cardiology. - : Elsevier. - 0022-2828 .- 1095-8584. ; 168, s. 107-114
  • Journal article (peer-reviewed)abstract
    • Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have advanced our ability to study the basic function of the heart and model cardiac diseases. Due to the complexities in stem cell culture and differentiation protocols, many researchers source their hiPSC-CMs from collaborators or commercial biobanks. Generally, the field has assumed the health of frozen cardiomyocytes is unchanged if the cells adhere to the substrate and commence beating. However, very few have investigated the effects of cryopreservation on hiPSCCM's functional and transcriptional health at the cellular and molecular level. Here we review methods and challenges associated with cryopreservation, and examine the effects of cryopreservation on the functionality (contractility and calcium handling) and transcriptome of hiPSC-CMs from six healthy stem cell lines. Utilizing protein patterning methods to template physiological cell aspect ratios (7:1, length:width) in conjunction with polyacrylamide (PA) hydrogels, we measured changes in force generation and calcium handling of single hiPSCCMs. We observed that cryopreservation altered the functionality and transcriptome of hiPSC-CMs towards larger sizes and contractile force as assessed by increased spread area and volume, single cell traction force microscopy and delayed calcium dynamics. hiPSC-CMs are broadly used for basic science research, regenerative medicine, and testing biological therapeutics. This study informs the design of experiments utilizing hiPSC-CMs to avoid confounding functional changes due to cryopreservation with other treatments.
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2.
  • Santangelo, James S., et al. (author)
  • Global urban environmental change drives adaptation in white clover
  • 2022
  • In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 375
  • Journal article (peer-reviewed)abstract
    • Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural dines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Tack, Ayco J. M. (1)
Andrew, Nigel R. (1)
Alberti, Marina (1)
Pinho, Pedro (1)
Puentes, Adriana (1)
Laine, Anna Liisa (1)
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Bonte, Dries (1)
del Val, Ek (1)
Branquinho, Cristina (1)
Peres-Neto, Pedro R. (1)
Granath, Gustaf (1)
Crawford, Andrew J. (1)
Zhao, Zhigang (1)
Classen, Aimée T. (1)
Sanders, Nathan J. (1)
Ellers, Jacintha (1)
Raeymaekers, Joost A ... (1)
Brans, Kristien, I (1)
Gagnon, Edeline (1)
Scheepens, J.F. (1)
Parachnowitsch, Amy ... (1)
Santangelo, James S. (1)
Ness, Rob W. (1)
Angeoletto, Fabio (1)
Anstett, Daniel N. (1)
Anstett, Julia (1)
Baena-Diaz, Fernanda (1)
Carlen, Elizabeth J. (1)
Chaves, Jaime A. (1)
Comerford, Mattheau ... (1)
Dyson, Karen (1)
Falahati-Anbaran, Mo ... (1)
Fellowes, Mark D. E. (1)
Hodgins, Kathryn A. (1)
Iñiguez-Armijos, Car ... (1)
Lázaro-Lobo, Adrián (1)
Moles, Angela T. (1)
Munshi-South, Jason (1)
Paule, Juraj (1)
Porth, Ilga M. (1)
Santiago-Rosario, Lu ... (1)
Whitney, Kaitlin Sta ... (1)
Johnson, Marc T. J. (1)
Rivas-Torres, Gonzal ... (1)
Chirikian, Orlando (1)
Feinstein, Samuel D. (1)
Faynus, Mohamed A. (1)
Kim, Anna A (1)
Lane, Kerry V. (1)
Torres, Gabriela V. (1)
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University
Uppsala University (2)
Stockholm University (1)
Lund University (1)
Swedish University of Agricultural Sciences (1)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (1)
Medical and Health Sciences (1)
Year
2022 (2)Undo refine

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