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- Stiel, H., et al.
(author)
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2D and 3D Nanoscale Imaging Using High Repetition Rate Laboratory-Based Soft X-Ray Sources
- 2018
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In: X-Ray Lasers 2016 - Proceedings of the 15th International Conference on X-Ray Lasers. - Cham : Springer International Publishing. - 9783319730240 ; 202, s. 265-272
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Conference paper (peer-reviewed)abstract
- In this contribution, we report about tomographic nanoscale imaging using a laser-produced plasma-based laboratory transmission X-ray microscope (LTXM) in the water window. The soft X-ray radiation of the LTXM is provided by a high average power laser-produced (1.3 kHz repetition rate, 0.5 ns pulse duration, 140 W average power) plasma source, a multilayer condenser mirror, an objective zone plate, and a back-illuminated CCD camera as a detector. In the second part of the contribution, we will present recent results on holography and coherent diffraction imaging using our high repetition rate X-ray laser. We will discuss advantages of these methods and its potential for nanoscale imaging.
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| 2. |
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| 3. |
- Tummler, C, et al.
(author)
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SYK Inhibition Potentiates the Effect of Chemotherapeutic Drugs on Neuroblastoma Cells in Vitro
- 2019
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In: Cancers. - : MDPI AG. - 2072-6694. ; 11:2
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Journal article (peer-reviewed)abstract
- Neuroblastoma is a malignancy arising from the developing sympathetic nervous system and the most common and deadly cancer of infancy. New therapies are needed to improve the prognosis for high-risk patients and to reduce toxicity and late effects. Spleen tyrosine kinase (SYK) has previously been identified as a promising drug target in various inflammatory diseases and cancers but has so far not been extensively studied as a potential therapeutic target in neuroblastoma. In this study, we observed elevated SYK gene expression in neuroblastoma compared to neural crest and benign neurofibroma. While SYK protein was detected in the majority of examined neuroblastoma tissues it was less frequently observed in neuroblastoma cell lines. Depletion of SYK by siRNA and the use of small molecule SYK inhibitors significantly reduced the cell viability of neuroblastoma cell lines expressing SYK protein. Moreover, SYK inhibition decreased ERK1/2 and Akt phosphorylation. The SYK inhibitor BAY 613606 enhanced the effect of different chemotherapeutic drugs. Transient expression of a constitutive active SYK variant increased the viability of neuroblastoma cells independent of endogenous SYK levels. Collectively, our findings suggest that targeting SYK in combination with conventional chemotherapy should be further evaluated as a treatment option in neuroblastoma.
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