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  • Kwiatkowski, Marek, et al. (author)
  • Facile Synthesis of N-Acyl-aminoacyl-pCpA for Preparation of Mischarged Fully Ribo tRNA
  • 2014
  • In: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 25:11, s. 2086-2091
  • Journal article (peer-reviewed)abstract
    • Chemical synthesis of N-acyl-aminoacyl-pdCpA and its ligation to tRNA(minus) CA is widely used for the preparation of unnatural aminoacyl-tRNA substrates for ribosomal translation. However, the presence of the unnatural deoxyribose can decrease incorporation yield in translation and there is no straightforward method for chemical synthesis of the natural ribo version. Here, we show that pCpA is surprisingly stable to treatment with strong organic bases provided that anhydrous conditions are used. This allowed development of a facile method for chemical aminoacylation of pCpA. Preparative synthesis of pCpA was also simplified by using t-butyl-dithiomethyl protecting group methodology, and a more reliable pCpA postpurification treatment method was developed. Such aminoacyl-pCpA analogues ligated to tRNA(minus) CA transcripts are highly active in a purified translation system, demonstrating utility of our synthetic method.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Wang, Jinfan (2)
Kwiatkowski, Marek (2)
Ehrenberg, Måns (1)
Forster, Anthony C. (1)
Pavlov, Michael Yu (1)
Forster, Anthony (1)
University
Uppsala University (2)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (2)
Year
2014 (2)Undo refine

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