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Search: WFRF:(Wange LE) > (2022)

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  • Janjic, A, et al. (author)
  • Prime-seq, efficient and powerful bulk RNA sequencing
  • 2022
  • In: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X. ; 23:1, s. 88-
  • Journal article (peer-reviewed)abstract
    • Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Richter, D. (2)
Nguyen, P (2)
Bagnoli, JW (2)
Ziegenhain, C (2)
Janjic, A (2)
Wange, LE (2)
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Vieth, B (2)
Geuder, J (2)
Hellmann, I (2)
Enard, W (2)
Vick, B (2)
Jeremias, I (2)
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University
Karolinska Institutet (2)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (1)
Medical and Health Sciences (1)
Year
2022 (2)Undo refine

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