| 1. |
- Humble, Elisabet, et al.
(author)
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Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide
- 1979
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In: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 90:3, s. 1064-1072
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Journal article (peer-reviewed)abstract
- Rat liver fructose-1,6-diphosphatase was phosphorylated with (32P)ATP and the catalytic subunit of cyclic AMP-dependent protein kinase from pig muscle. After digestion with pepsin, α-chymotrypsin and subtilisin a peptide with the amino-terminal sequence Ser-Arg-Tyr-(32P)SerP-Leu-Pro-Leu-Pro was isolated. A synthetic unphosphorylated heptapeptide with the same amino acid sequence, ending with leucine, was phosphorylated with an apparent Km of 400 μM, while the apparent Km value for fructose-1,6-diphosphatase was 30 μM (subunit concentration). The Vmax value was 20 times higher for the peptide than for the enzyme.
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| 2. |
- Wassenius, Ola, et al.
(author)
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Variability in Skin Exposure in Machine Operators Exposed to Cutting Fluids
- 1998
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In: Scandinavian Journal of Work, Environment and Health. - : Scandinavian Journal of Work, Environment and Health. - 0355-3140 .- 1795-990X. ; 24:2, s. 125 - 129
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Journal article (peer-reviewed)abstract
- Objective. This study describes a new technique for measuring skin exposure to cutting fluids and evaluates the variability of skin exposure among machine operators performing cyclic (repetitive) work. Methods. The technique is based on video recording and subsequent analysis of the videotape by means of computer-synchronized video equipment. The time intervals at which the machine operator's hand was exposed to fluid were registered, and the total wet time of the skin was calculated by assuming different evaporation times for the fluid. The exposure of twelve operators with different work methods was analyzed in six different workshops, which included a range of machine types, from highly automated metal cutting machines (ie, actual cutting and chip removal machines) requiring operator supervision to conventional metal cutting machines, where the operator was required to maneuver the machine and manually exchange products. Results; the relative wet time varied between 0% and 100%. A significant association between short cycle time and high relative wet time was noted. However, there was no relationship between the degree of automatization of the metal cutting machines and wet time. Conclusions. The study shows that skin exposure to cutting fluids can vary considerably between machine operators involved in manufacturing processes using different types of metal cutting machines. The machine type was not associated with dermal wetness. The technique appears to give objective information about dermal wetness. A comment: This publication is a result of a cooperation between the authors at Chalmers University of Technology and Gothenburg University, i.e. between the Department of Transportation and the Department of Occupational Health and the Department of Sociology.
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| 7. |
- Björnstig, Ulf
(author)
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Snowmobile, motorcycle and moose-car accidents : aspects on injury control
- 1985
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Doctoral thesis (other academic/artistic)abstract
- Injuries related to snowmobiles, motorcycles and moose-car collisions have increased. The aim of the present thesis was to analyse mechanisms and consequences in fatal and non-fatal accidents of these types, and to penetrate possible preventive measures.SnowmobilesThe median age of the injured was 30 and of the killed 32, males predominating. A majority of the accidents occurred during weekends, and especially the fatal accidents occurred after dark. The extremities were the most commonly injured parts of the body, however, drowning, crushed chest and cranial injuries caused most of the fatalities. Among the fatalities, four out of five were under the influence of alcohol. The present Swedish laws regarding snowmobiles seem well motivated. "Built-in" safety measures in the construction of the snowmobiles, properly designed snowmobile tracks and functional search and rescue systems could reduce the injuries.MotorcyclesThe median age was 19 years for both the traffic injured and the killed. Half of the traffic accidents were collisions with other motor vehicles. In the fatally injured group, also collisions with fixed roadside objects were common. Of the fatally injured, more than every fifth person died in an accident where alcohol was an influential factor. In the injured group, lower extremity injuries (especially in off-road riding) were common and among the fatalities most riders died from injuries to the head or chest. Out of one thousand motorcycle riders interviewed, 45% reported wobbling experiences (8% reported severe wobbling). Possible injury reducing measures include increasing the licensing age, more discriminating driving test, "built-in" restriction of the motorcycle's top speed, elimination of motorcycles prone to wobbling, and a more intensive traffic supervision (speeding, alcohol).Moose-car collisitionsThe median age of the drivers was 38 years. Most collisions happened at dusk or when dark (3/4). The median collision speed was 70 km/h. The damage to the car was typical, the roof and the windshield pillars were deformed downwards and backwards. The broken windshield was often pressed into the passenger compartment. Most of the injured car occupants suffered cuts predominantly to the head and upper extremities. Nearly all the fatally injured died of head and neck injuries. The injuries may be reduced by strengthening the roof and the windshield pillars, and by introducing anti 1 acerati ve windshields.
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| 8. |
- Bruce, Barbro, et al.
(author)
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Can severe language disorders be identified before age 3;6?
- 1996
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In: Logopedics Phoniatrics Vocology. - : Informa UK Limited. - 1401-5439 .- 1651-2022. ; 21:1, s. 37-42
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Journal article (peer-reviewed)abstract
- A new classification system indicating type and severity of language disorder was applied by five speech pathologists. They independently interpreted information from the initial language assessment of 39 children with developmental language disorder (DLD), who had been assessed by one of the present authors before age 3;6. Information about planned intervention was also collected. The children were reassessed 18 months later. The amount of language therapy the children had received was calculated and related to the type and severity of the language disorder. Our results indicate that the DLD children, who initially had general language problems performed worse on reassessment than children with expressive problems. More extensive intervention programmes were planned for the children with general DLD. According to follow-up data they had, however, not received more therapy than children with more restricted disorders.
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| 9. |
- Dahlqvist-Edberg, Ulla, et al.
(author)
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Purification of a Ca2+-activated protease from rat erythrocytes and its possible effect on pyruvate kinase in vivo
- 1981
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In: Biochimica et Biophysica Acta-Enzymology. - : Elsevier BV. - 0005-2744. ; 660:1, s. 96-101
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Journal article (peer-reviewed)abstract
- A Ca2+-activated protease with [32P]phosphopyruvate kinase as substrate was purified to about 50% from rat erythrocytes. The purification involved chromatography on Sepharose/Sephadex gels, DEAE-cellulose and (NH4)2SO4 precipitation. The protease required 3.3 mM Ca2+ for full activity. When pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) was purified from erythrocytes incubated with [32P]phosphate it contained 0.5 mol [32P]phosphate/mol enzyme subunit. When 3.3 mM Ca2+ were added at hemolysis this incorporation decreased. The possible importance of this Ca2+-activated protease for the regulation of pyruvate kinase in erythrocytes is discussed.
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| 10. |
- Edvardsson, Bo, 1944-
(author)
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"Mina hypoteser om Stern tycks vara riktiga!"
- 1986
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In: Personal, människor & arbete. - Stockholm : Sveriges personaladministrativa förening. - 0348-5242. ; :2, s. 36-36
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Journal article (pop. science, debate, etc.)abstract
- En uppföljande debattartikel efter replikerna på mitt debattinlägg i nr 9, 1985
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