| 11. |
- Abrahamson, Magnus, et al.
(author)
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The human cystatin C gene (CST3), mutated in hereditary cystatin C amyloid angiopathy, is located on chromosome 20
- 1989
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In: Human Genetics. - 1432-1203. ; 82:3, s. 223-226
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Journal article (peer-reviewed)abstract
- Hereditary cystatin C amyloid angiopathy has recently been shown to be caused by a point mutation in the cystatin C gene. To determine the chromosomal localization of the gene, 20 human-rodent somatic cell hybrids and a fulllength cystatin C cDNA probe were used. Southern blot analysis of BamHI digested cell hybrid DNA revealed that the probe recognizes a 10.6 kb human specific fragment and that this fragment cosegregates with human chromosome 20. Therefore, the human cystatin C gene (CST3) was assigned to chromosome 20.
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| 12. |
- Abrahamsson, P. ‐A, et al.
(author)
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Immunohistochemical distribution of the three predominant secretory proteins in the parenchyma of hyperplastic and neoplastic prostate glands
- 1988
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In: The Prostate. - : Wiley. - 0270-4137 .- 1097-0045. ; 12:1, s. 39-46
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Journal article (peer-reviewed)abstract
- Prostatic acid phosphatase (PAP), prostate‐specific antigen (PSA), and β‐microseminoprotein (β‐MSP) were regularly localized immunohistochemically to the epithelium of the acini and that of the ducts in the nodules of 24 cases of benign prostatic hyperplasia. The immunohistochemical distribution of these three prostatic‐secreted proteins was also examined, with monoclonal antisera against PAP and PSA and with polyclonal antisera against PAP, PSA, and β‐MSP, in a series of 40 cases of prostatic adenocarcinomas graded according to the WHO classification. Highly differentiated (grade I) carcinomas showed a high incidence of PAP‐, PSA‐, and β‐MSP‐immunoreactive cells. As in the normal and hyperplastic prostate parenchyma, highly differentiated (grade I) carcinomas were found to contain an almost equal number of PAP‐, PSA‐, and β‐MSP‐immunoreactive cells. When semiquantitatively assessed, the incidence of PAP‐, PSA‐, and β‐MSP‐immunoreactive cells was found to be lower in the moderately and poorly differentiated (grades II and III) tumors than in the highly differentiated ones; they also showed greater staining variability. Tumor cells immunoreactive with a monoclonal antiserum raised against PAP in carcinomas of grades II and III were less frequent than tumor cells immunoreactive with antisera against PSA, β‐MSP, and a polyclonal antiserum against PAP. The almost identical distribution of PSA and β‐MSP in carcinomas of grades II and III suggests that PSA and β‐MSP are not less sensitive tumor markers than PAP for the monitoring of the course and the treatment of prostatic carcinomas.
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| 13. |
- Abrahamsson, P. ‐A, et al.
(author)
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Partial characterization of a thyroid‐stimulating hormone‐like peptide in neuroendocrine cells of the human prostate gland
- 1989
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In: The Prostate. - : Wiley. - 0270-4137 .- 1097-0045. ; 14:1, s. 71-81
-
Journal article (peer-reviewed)abstract
- Immunohistochemical identification of the most prevalent type of neuroendocrine (NE) cells in the human prostate gland can be made with polyclonal antisera against human thyroid‐stimulating hormone (TSH). A TSH‐like peptide was characterized by analysis of prostatic tissue homogenates with sodium dodecyl sulfate‐polyacrylamide gel (SDS‐PAGE) electrophoresis followed by immunoblotting. A single protein band, with an apparent mass of about 32 kDa after reduction, was identified both with polyclonal antisera against human TSH and with a polyclonal antiserum raised against a synthetic peptide corresponding to the carboxyterminal part of the β‐subunit of human TSH. The TSH‐like prostatic peptide identified here is, on the basis of its molecular mass and absence of immunoreactivity with an antiserum raised against a synthetic peptide representing the mid‐portion of the β‐subunit of TSH, not identical with the pituitary β‐subunit of TSH. On the other hand, this 32 kDa prostatic peptide may have certain structural elements in common with the pituitary β‐subunit of TSH, since it is recognized both with polyclonal antisera against TSH and with an antiserum against the carboxyterminal part of the β‐subunit of TSH.
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| 14. |
- Abrahamsson, Per-Anders, et al.
(author)
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Radioimmunoassay of beta-microseminoprotein, a prostatic-secreted protein present in sera of both men and women
- 1989
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In: Clinical Chemistry. - 0009-9147. ; 35:7, s. 1497-1503
-
Journal article (peer-reviewed)abstract
- We describe a simple radioimmunoassay of beta-microseminoprotein, one of the three most abundant secretory proteins of the prostate gland. The detection limit of the assay is 1 microgram/L, and its precision, expressed as the total coefficient of variation, is less than 10% for values between 10 and 150 micrograms/L. Using this assay, we found that beta-microseminoprotein immunoreactivity was present in sera from both sexes at about the same concentration. The protein detected had the same molecular size on gel chromatography as the protein isolated from seminal plasma, and dilution curves for the sera paralleled that for the pure protein. The findings suggest that beta-microseminoprotein is present in serum of healthy subjects of both sexes and that it originates in tissue other than the prostate gland. The range of the serum concentration was 0-10.6 micrograms/L (median 4.1) for 51 healthy adult women and 1.1-14.7 micrograms/L (median 6.2) for 35 healthy adult men not older than 40 years. In males with prostatic cancer the concentration in serum was highly variable and often greatly increased. The concentration of beta-microseminoprotein was correlated with that of creatinine in serum, suggesting that the protein is eliminated--at least partly--from the circulation by glomerular filtration. Little of the protein was present in the urine of women. In urine from men the concentration was high and variable, probably because of local contribution from the prostate gland to the urethral urine.
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| 15. |
- Abramson, N, et al.
(author)
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Light-in-flight recording. 5: Theory of slowing down the faster-than-light motion of the light shutter
- 1989
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In: Applied Optics. - 2155-3165. ; 28:4, s. 759-765
-
Journal article (peer-reviewed)abstract
- Light-in-flight recording by holography uses a picosecond pulse for the reference beam, which like a sheet of light intersects the hologram plate and produces a sensitivity area that with a speed faster than light moves over the plate like a light shutter. If, however, the front of the reference pulse by diffraction in a grating is tilted relative to its direction of motion, the velocity of the light shutter can be slowed down resulting in increased recording time. The practical result using a reflection grating was a true recording that corresponded to a time compression of two to one. To minimize distortions of the recorded pulse shape we studied intersections that are identical for apparent (ellipsoidal) and true (spheroidal) wavefronts.
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