| 1. |
- Liu, Yuanhua, 1971, et al.
(author)
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Considering the importance of user profiles in interface design
- 2009
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In: User Interfaces. ; , s. 23-
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Book chapter (other academic/artistic)abstract
- User profile is a popular term widely employed during product design processes by industrial companies. Such a profile is normally intended to represent real users of a product. The ultimate purpose of a user profile is actually to help designers to recognize or learn about the real user by presenting them with a description of a real user’s attributes, for instance; the user’s gender, age, educational level, attitude, technical needs and skill level. The aim of this chapter is to provide information on the current knowledge and research about user profile issues, as well as to emphasize the importance of considering these issues in interface design. In this chapter, we mainly focus on how users’ difference in expertise affects their performance or activity in various interaction contexts. Considering the complex interaction situations in practice, novice and expert users’ interactions with medical user interfaces of different technical complexity will be analyzed as examples: one focuses on novice and expert users’ difference when interacting with simple medical interfaces, and the other focuses on differences when interacting with complex medical interfaces. Four issues will be analyzed and discussed: (1) how novice and expert users differ in terms of performance during the interaction; (2) how novice and expert users differ in the perspective of cognitive mental models during the interaction; (3) how novice and expert users should be defined in practice; and (4) what are the main differences between novice and expert users’ implications for interface design. Besides describing the effect of users’ expertise difference during the interface design process, we will also pinpoint some potential problems for the research on interface design, as well as some future challenges that academic researchers and industrial engineers should face in practice.
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| 2. |
- Fredenberg, Erik, 1979-
(author)
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Spectral Mammography with X-Ray Optics and a Photon-Counting Detector
- 2009
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Doctoral thesis (other academic/artistic)abstract
- Early detection is vital to successfully treating breast cancer, and mammography screening is the most efficient and wide-spread method to reach this goal. Imaging low-contrast targets, while minimizing the radiation exposure to a large population is, however, a major challenge. Optimizing the image quality per unit radiation dose is therefore essential. In this thesis, two optimization schemes with respect to x-ray photon energy have been investigated: filtering the incident spectrum with refractive x-ray optics (spectral shaping), and utilizing the transmitted spectrum with energy-resolved photon-counting detectors (spectral imaging). Two types of x-ray lenses were experimentally characterized, and modeled using ray tracing, field propagation, and geometrical optics. Spectral shaping reduced dose approximately 20% compared to an absorption-filtered reference system with the same signal-to-noise ratio, scan time, and spatial resolution. In addition, a focusing pre-object collimator based on the same type of optics reduced divergence of the radiation and improved photon economy by about 50%. A photon-counting silicon detector was investigated in terms of energy resolution and its feasibility for spectral imaging. Contrast-enhanced tumor imaging with a system based on the detector was characterized and optimized with a model that took anatomical noise into account. Improvement in an ideal-observer detectability index by a factor of 2 to 8 over that obtained by conventional absorption imaging was found for different levels of anatomical noise and breast density. Increased conspicuity was confirmed by experiment. Further, the model was extended to include imaging of unenhanced lesions. Detectability of microcalcifications increased no more than a few percent, whereas the ability to detect large tumors might improve on the order of 50% despite the low attenuation difference between glandular and cancerous tissue. It is clear that inclusion of anatomical noise and imaging task in spectral optimization may yield completely different results than an analysis based solely on quantum noise.
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| 3. |
- Löfhede, Johan, et al.
(author)
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Comparing a Supervised and an Unsupervised Classification Method for Burst Detection in Neonatal EEG
- 2008
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In: Proceedings of Engineering in Medicine and Biology Society, EMBS 2008. 30th Annual International Conference of the IEEE, 20-24 August, 2008. - : IEEE. - 1557-170X. - 9781424418145 ; , s. 3836-3839
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Conference paper (peer-reviewed)abstract
- Hidden Markov Models (HMM) and Support Vector Machines (SVM) using unsupervised and supervised training, respectively, were compared with respect to their ability to correctly classify burst and suppression in neonatal EEG. Each classifier was fed five feature signals extracted from EEG signals from six full term infants who had suffered from perinatal asphyxia. Visual inspection of the EEG by an experienced electroencephalographer was used as the gold standard when training the SVM, and for evaluating the performance of both methods. The results are presented as receiver operating characteristic (ROC) curves and quantified by the area under the curve (AUC). Our study show that the SVM and the HMM exhibit similar performance, despite their fundamental differences.
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| 4. |
- Munthe, Christian, 1962
(author)
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Etiska aspekter på regenerativ medicin : Ethical aspects on regenerative medicine
- 2003
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In: SNIB-konferensen 2003, Chalmers tekniska högskola, Göteborg, 16-18 maj 2003.
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Conference paper (other academic/artistic)abstract
- Inom den regenerativa medicinen strävar man efter att ersätta skadat eller sjukligt biologiskt mänskligt material (celler, organ, kroppsdelar) med nya biologiska komponenter. Området aktualiserar en rad etiska frågeställningar vad gäller (1) produktionen av ersättningsmaterialet (t.ex. embryonala stamceller eller införskaffande av transplantationsvävnad från donatorer), (2) risker i samband med försök på människa (genmodifierat material, material från djur), samt (3) gränserna för hur långt man bör gå i denna slags försök att förlänga människans livsspann. Föredraget ger en kort översikt över dessa frågeställningar, ståndpunkter och argument i debatten kring dem.
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| 5. |
- Fredenberg, Erik, PhD, 1979-, et al.
(author)
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A low-absorption x-ray energy filter for small-scale applications
- 2009
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In: Optics Express. - : The Optical Society. - 1094-4087. ; 17:14, s. 11388-11398
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Journal article (peer-reviewed)abstract
- We present an experimental and theoretical evaluation of an x-ray energy filter based on the chromatic properties of a prism-array lens (PAL). It is intended for small-scale applications such as medical imaging. The PAL approximates a Fresnel lens and allows for high efficiency compared to filters based on ordinary refractive lenses, however at the cost of a lower energy resolution. Geometrical optics was found to provide a good approximation for the performance of a flawless lens, but a field-propagation model was used for quantitative predictions. The model predicted a 0.29 ΔE/E energy resolution and an intensity gain of 6.5 for a silicon PAL at 23.5 keV. Measurements with an x-ray tube showed good agreement with the model in energy resolution and peak energy, but a blurred focal line contributed to a 29% gain reduction. We believe the blurring to be caused mainly by lens imperfections, in particular at the periphery of the lens.
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| 6. |
- Fredenberg, Erik, PhD, 1979-, et al.
(author)
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A Tunable Energy Filter for Medical X-Ray Imaging
- 2008
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In: X-Ray Optics and Instrumentation. - : Hindawi. - 1687-7632 .- 1687-7640. ; 2008
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Journal article (peer-reviewed)abstract
- A multiprism lens (MPL) is a refractive X-ray lens, and its chromatic properties can be employed in an energy filtering setup to obtain a narrow tunable X-ray spectrum. We present the first evaluation of such a filter for medical X-ray imaging. The experimental setup yields a 6.6 gain of flux at 20 keV, and we demonstrate tunability by altering the energy spectrum to center also around 17 and 23 keV. All measurements are found to agree well with ray-tracing and a proposed geometrical model. Compared to a model mammography system with absorption filtering, the experimental MPL filter reduces dose 13–25% for 3–7 cm breasts if the spectrum is centered around the optimal energy. Additionally, the resolution is improved 2.5 times for a 5 cm breast. The scan time is increased 3 times but can be reduced with a slightly decreased energy filtering and resolution.
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| 7. |
- Löfhede, Johan, et al.
(author)
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Classification of burst and suppression in the neonatal electroencephalogram
- 2008
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In: Journal of Neural Engineering. - : Institute of Physics Publishing Ltd.. - 1741-2560 .- 1741-2552. ; 5:4, s. 402-410
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Journal article (peer-reviewed)abstract
- Fisher's linear discriminant (FLD), a feed-forward artificial neural network (ANN) and a support vector machine (SVM) were compared with respect to their ability to distinguish bursts from suppressions in electroencephalograms (EEG) displaying a burst-suppression pattern. Five features extracted from the EEG were used as inputs. The study was based on EEG signals from six full-term infants who had suffered from perinatal asphyxia, and the methods have been trained with reference data classified by an experienced electroencephalographer. The results are summarized as the area under the curve (AUC), derived from receiver operating characteristic (ROC) curves for the three methods. Based on this, the SVM performs slightly better than the others. Testing the three methods with combinations of increasing numbers of the five features shows that the SVM handles the increasing amount of information better than the other methods.
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| 8. |
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| 9. |
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| 10. |
- Thordstein, Magnus, et al.
(author)
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Effects of inflammation on cerebral electric activity in fetal sheep
- 2008
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In: 2nd Congress of the European Academy of Paediatrics, Nice 23-28 okt 2008.
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Conference paper (other academic/artistic)abstract
- OBJECTIVE Intrauterine infections can by themselves induce fetal brain damage but also potentiate the effects of other harmful influences such as asphyxia and seizures. Using an EEG technique that permits the recording of extremely low frequencies, often called DC EEG, changes in the level, i.e. DC shifts can be detected. The DC level has been suggested to depend mainly on the potential over the blood brain barrier (BBB), in turn decided primarily by the arterial level of pCO2. Fetuses affected by infection/inflammation that produce detrimental effects on the brain, may have elevated levels of pCO2 and disturbance of the BBB. We aimed at investigating the possibility that the DC EEG could be used to detect the effects of inflammation on the fetal brain. METHODS Fetal sheep were instrumented at 97 days of gestation with catheters, four active EEG electrodes placed on the dura mater as well as extracranial reference and ground electrodes. After three days of recovery, the bacterial endotoxin lipopolysaccharide (LPS) was given to the fetus (200 ng i.v.). RESULTS Exposure to LPS induced a positive DC shift in parallel to the assumed affection of cerebral function and to the pCO2 elevation. This change was not always obvious in standard EEG. CONCLUSIONS These recordings of fetal DC EEG appear to be the first to be done. They indicate that the effects of inflammation on cerebral function can be monitored by DC EEG. Such monitoring might be feasible also during late stages of labour and in neonates.
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| 11. |
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| 12. |
- Löfhede, Johan, 1978, et al.
(author)
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Detection of bursts in the EEG of post asphyctic newborns
- 2006
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In: 2006 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. - 1557-170X. - 9781424400324 ; , s. 2179-2182, s. 5-6
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Conference paper (peer-reviewed)abstract
- Eight features inherent in the electroencephalogram (EEG) have been extracted and evaluated with respect to their ability to distinguish bursts from suppression in burst-suppression EEG. The study is based on EEG from six full term infants who had suffered from lack of oxygen during birth. The features were used as input in a neural network, which was trained on reference data segmented by an experienced electroencephalographer. The performance was then evaluated on validation data for each feature separately and in combinations. The results show that there are significant variations in the type of activity found in burst-suppression EEG from different subjects, and that while one or a few features seem to be sufficient for most patients in this group, some cases require specific combinations of features for good detection to be possible.
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| 13. |
- Liu, Yuanhua, 1971
(author)
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Usability Evaluation of Medical Technology: Investigating the Effect of User Background and Users' Expertise
- 2009
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Doctoral thesis (other academic/artistic)abstract
- Medical devices play a major role in diagnosis and therapeutics in the healthcare systems. The basic features of medical devices concern safety and efficient clinical performance. Nowadays, usability evaluation is an important part of the design process of medical devices. The aims of this thesis work were to investigate the effect of user profiles on usability evaluation results and to provide practical advice on choice of users as test subjects when conducting usability evaluations of medical devices. Five studies, with analytical and empirical evaluation approaches with different foci, were included in the work. In the studies, consideration was given to user background and users’ expertise as well as to user interfaces of different levels of complexity. The user background aspect was taken as the focus for the analytical evaluation approach, while users’ expertise was taken as the focus for the empirical evaluation approach. Cognitive Walkthrough was employed as an example of an analytical evaluation method to investigate user background, while usability tests were used as an example of empirical evaluation method to investigate users’ expertise. The results showed that medical device user background settings can influence the outcome of an analytical evaluation results, i.e. when more ergonomic factors were included in the user background settings, a wider range of usability problems were detected. User expertise is an important factor for the results of empirical usability tests. Users’ familiarity with tasks can be used as an important criterion for classification of user expertise. The quantitative analysis of the empirical evaluations implies that the effect of users’ expertise may be invisible when interacting with a simple user interface, but visible when interacting with a more complex user interface. Expert users outperformed novice users when interacting with a complex interface but not when interacting with a simple interface.The qualitative analysis of verbal explanations and statements, causes of errors and redesign proposals stressed the differences between novice and expert users in terms of decision-making, presentation and judgment, which implied that expert users’ use experience and novice users’ interaction experience differ in contributing to product design and development. Insufficient domain and interaction knowledge were consistently identified as typical causes of errors for novice users. The differences of information organization between previously experienced user interfaces and the interfaces interacted in the usability tests affected expert users’ task completion. Expert users made task-related errors due to terminology issues and interaction-related errors due to their ‘old’ mental model of how to interact with the user interface. Based on the results, different strategies are suggested to be used when choosing test subjects for usability evaluations in different interaction situations. A guideline and some practical advices were proposed as well to medical industrial companies about how to conduct usability evaluations on medical devices.
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| 14. |
- Eklund, Anders, et al.
(author)
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Evaluation of applanation resonator sensors for intra-ocular pressure measurement : results from clinical and in vitro studies.
- 2003
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In: Medical and Biological Engineering and Computing. - 0140-0118 .- 1741-0444. ; 41:2, s. 190-197
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Journal article (peer-reviewed)abstract
- Glaucoma is an eye disease that, in its most common form, is characterised by high intra-ocular pressure (IOP), reduced visual field and optic nerve damage. For diagnostic purposes and for follow-up after treatment, it is important to have simple and reliable methods for measuring IOP. Recently, an applanation resonator sensor (ARS) for measuring IOP was introduced and evaluated using an in vitro pig-eye model. In the present study, the first clinical evaluation of the same probe has been carried out, with experiments in vivo on human eyes. There was a low but significant correlation between IOP(ARS) and the IOP measured with a Goldmann applanation tonometer (r = 0.40, p = 0.001, n = 72). However, off-centre positioning of the sensor against the cornea caused a non-negligible source of error. The sensor probe was redesigned to have a spherical, instead of flat, contact surface against the eye and was evaluated in the in vitro model. The new probe showed reduced sensitivity to off-centre positioning, with a decrease in relative deviation from 89% to 11% (1 mm radius). For normalised data, linear regression between IOP(ARS) and direct IOP measurement in the vitreous chamber showed a correlation of r = 0.97 (p < 0.001, n = 108) and a standard deviation for the residuals of SD < or = 2.18 mm Hg (n = 108). It was concluded that a spherical contact surface should be preferred and that further development towards a clinical instrument should focus on probe design and signal analysis.
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| 15. |
- Fredenberg, Erik, 1979-, et al.
(author)
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An efficient pre-object collimator based on an x-ray lens
- 2009
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In: Medical physics (Lancaster). - : Wiley. - 0094-2405. ; 36:2, s. 626-633
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Journal article (peer-reviewed)abstract
- A multiprism lens (MPL) is a refractive x-ray lens with one-dimensional focusing properties. If used as a pre-object collimator in a scanning system for medical x-ray imaging, it reduces the divergence of the radiation and improves on photon economy compared to a slit collimator. Potential advantages include shorter acquisition times, a reduced tube loading, or improved resolution. We present the first images acquired with a MPL in a prototype for a scanning mammography system. The lens showed a gain of flux of 1.32 compared to a slit collimator at equal resolution, or a gain in resolution of 1.31–1.44 at equal flux. We expect the gain of flux in a clinical setup with an optimized MPL and a custom-made absorption filter to reach 1.67, or 1.45–1.54 gain in resolution.
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| 16. |
- Buendia, Ruben, 1982, et al.
(author)
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A Novel Approach for Removing the Hook Effect Artefact from Electrical Bioimpedance Spectroscopy Measurements
- 2009
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In: Journal of Physics: Conference Series. - : Institute of Physics Publishing Ltd.. - 1742-6596.
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Conference paper (peer-reviewed)abstract
- Very often in Electrical Bioimpedance (EBI) spectroscopy measurements the presence of stray capacitances creates a measurement artefact commonly known as Hook Effect. Such an artefact creates a hook-alike deviation of the EBI data noticeable when representing the measurement on the impedance plane. Such Hook Effect is noticeable at high frequencies but it also causes a data deviation at lower measurement frequencies. In order to perform any accurate analysis of the EBI spectroscopy data, the influence of the Hook Effect must be removed. An established method to compensate the hook effect is the well known Td compensation, which consist on multiplying the obtained spectrum, Zmeas() by a complex exponential in the form of exp[jTd]. Such a method cannot correct entirely the Hook Effect since the hook-alike deviation occurs a broad frequency range in both magnitude and phase of the measured impedance, and by using a real value for Td. First, a real value only modifies the phase of the measured impedance and second, it can only correct the Hook Effect at a single frequency. In addition, the process to select a value for Td by an iterative process with the aim to obtain the best Cole fitting lacks solid scientific grounds. In this work the Td compensation method is revisited and a modified approach for correcting the Hook Effect that includes a novel method for selecting the correcting values is proposed. The initial validation results confirm that the proposed method entirely corrects the Hook Effect at all frequencies.
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| 17. |
- Axelberg, Peter
(author)
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On Tracing Flicker Sources and Classification of Voltage Disturbances
- 2007
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Doctoral thesis (other academic/artistic)abstract
- Developments in measurement technology, communication and data storage have resulted in measurement systems that produce large amount of data. Together with the long existing need for characterizing the performance of the power system this has resulted in demand for automatic and efficient information-extraction methods. The objective of the research work presented in this thesis was therefore to develop new robust methods that extract additional information from voltage and current measurements in power systems. This work has contributed to two specific areas of interest. The first part of the work has been the development of a measurement method that gives information how voltage flicker propagates (with respect to a monitoring point) and how to trace a flicker source. As part of this work the quantity of flicker power has been defined and integrated in a perceptionally relevant measurement method. The method has been validated by theoretical analysis, by simulations, and by two field tests (at low-voltage and at 130-kV level) with results that matched the theory. The conclusion of this part of the work is that flicker power can be used for efficient tracing of a flicker source and to determine how flicker propagates. The second part of the work has been the development of a voltage disturbance classification system based on the statistical learning theory-based Support Vector Machine method. The classification system shows always high classification accuracy when training data and test data originate from the same source. High classification accuracy is also obtained when training data originate from one power network and test data from another. The classification system shows, however, lower performance when training data is synthetic and test data originate from real power networks. It was concluded that it is possible to develop a classification system based on the Support Vector Machine method with “global settings” that can be used at any location without the need to retrain. The conclusion is that the proposed classification system works well and shows sufficiently high classification accuracy when trained on data that originate from real disturbances. However, more research activities are needed in order to generate synthetic data that have statistical characteristics close enough to real disturbances to replace actual recordings as training data.
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| 18. |
- Seoane Martinez, Fernando, 1976
(author)
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Electrical Bioimpedance Cerebral Monitoring: Fundamental Steps towards Clinical Application
- 2007
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Doctoral thesis (other academic/artistic)abstract
- Neurologically related injuries cause a similar number of deaths as cancer, and brain damage is the second commonest cause of death in the world and probably the leading cause of permanent disability. The devastating effects of most cases of brain damage could be avoided if it were detected and medical treatment initiated in time. The passive electrical properties of biological tissue have been investigated for almost a century and electrical bioimpedance studies in neurology have been performed for more than 50 years. Even considering the extensive efforts dedicated to investigating potential applications of electrical bioimpedance for brain monitoring, especially in the last 20 years, and the specifically acute need for such non-invasive and efficient diagnosis support tools, Electrical Bioimpedance technology has not made the expected breakthrough into clinical application yet. In order to reach this stage in the age of evidence-based medicine, the first essential step is to demonstrate the biophysical basis of the method under study. The present research work confirms that the cell swelling accompanying the hypoxic/ischemic injury mechanism modifies the electrical properties of brain tissue, and shows that by measuring the complex electrical bioimpedance it is possible to detect the changes resulting from brain damage. For the development of a successful monitoring method, after the vital biophysical validation it is critical to have available the proper electrical bioimpedance technology and to implement an efficient protocol of use. Electronic instrumentation is needed for broadband spectroscopy measurements of complex electrical bioimpedance; the selection of the electrode setup is crucial to obtain clinically relevant measurements, and the proper biosignal analysis and processing is the core of the diagnosis support system. This work has focused on all these aspects since they are fundamental for providing the solid medico-technological background necessary to enable the clinical usage of Electrical Bioimpedance for cerebral monitoring.
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| 19. |
- Hallberg, Josef, et al.
(author)
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Enriched media-experience of sport events
- 2004
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In: Proceedings. - Los Alamitos, Calif : IEEE Communications Society. - 0769522580 ; , s. 2-9
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Conference paper (peer-reviewed)abstract
- This paper describes a system where Internet-enabled sensor technology was integrated into a context-aware platform to give viewers of sport events an enriched media experience. The system was developed as a proof of concept and was evaluated during real-life use at the Vasaloppet cross-country ski event. Using Bluetooth wireless ad-hoc networking and GPRS technology, sensor data was transmitted from contestants to the context-aware platform Alipes, which in turn presented the sport event viewer with a personalized, context-aware view. In this paper we discuss the system architecture and integration of components. The system was evaluated both from technical and user perspectives, where the evaluation results confirm our approach to be technically feasible and that the system provide an enriched media-experience for the majority of viewers.
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| 20. |
- Svensson, Sara, et al.
(author)
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Cross-country skiers go on-line
- 2006
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Reports (other academic/artistic)abstract
- This article presents a pervasive computing system which give spectators of sport events an enriched media experience. The system utilizes Internet- enabled sensor technology integrated into a context-aware platform and was evaluated during real-life use at the Vasaloppet cross-country ski event. Sensor data was transmitted from contestants, using Bluetooth wireless ad- hoc networking and GPRS technology, to the context-aware platform which in turn presented the sport event spectator with a personalized and context- aware view. The system architecture and integration of components are discussed in this article, together with evaluations from technical and user perspectives. The results confirm that our approach is technically feasible and that the system provides an enriched media-experience for the majority of spectators.
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| 21. |
- Dyverfeldt, Petter, 1980-, et al.
(author)
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Quantification of intravoxel velocity standard deviation and turbulence intensity by generalizing phase-contrast MRI
- 2006
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In: Magnetic Resonance in Medicine. - : Wiley. - 0740-3194 .- 1522-2594. ; 56:4, s. 850-858
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Journal article (peer-reviewed)abstract
- Turbulent flow, characterized by velocity fluctuations, is a contributing factor to the pathogenesis of several cardiovascular diseases. A clinical noninvasive tool for assessing turbulence is lacking, however. It is well known that the occurrence of multiple spin velocities within a voxel during the influence of a magnetic gradient moment causes signal loss in phase-contrast magnetic resonance imaging (PC-MRI). In this paper a mathematical derivation of an expression for computing the standard deviation (SD) of the blood flow velocity distribution within a voxel is presented. The SD is obtained from the magnitude of PC-MRI signals acquired with different first gradient moments. By exploiting the relation between the SD and turbulence intensity (TI), this method allows for quantitative studies of turbulence. For validation, the TI in an in vitro flow phantom was quantified, and the results compared favorably with previously published laser Doppler anemometry (LDA) results. This method has the potential to become an important tool for the noninvasive assessment of turbulence in the arterial tree.
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| 22. |
- Prikulis, Juris, 1973, et al.
(author)
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Optical spectroscopy of single trapped metal nanoparticles in solution
- 2004
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In: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 4:1, s. 115-118
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Journal article (peer-reviewed)abstract
- We present a simple method for the optical manipulation and spectroscopy of colloidal silver nanoparticles in aqueous solution using optical tweezers combined with dark-field microscopy. Because of their localized plasmon resonances, single trapped metal nanoparticles can be used as efficient near-field optical probes, with potential applicability in surface-enhanced spectroscopy, near-field microscopy, and biochemical sensing schemes. As a proof of principle, we study the near-field optical interaction between a trapped and an immobilized Ag nanoparticle.
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| 23. |
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| 24. |
- Bitaraf, Nazanin, et al.
(author)
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Development of a multifunctional microfluidic system for studies of nerve cell activity during hypoxic and anoxic conditions
- 2009
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In: World Congress on Medical Physics and Biomedical Engineering. - Berlin : Springer Science+Business Media B.V.. - 9783642038976 ; , s. 176-179, s. 176-179
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Conference paper (peer-reviewed)abstract
- Hemoproteins usually supply cells and tissue with oxygen. A new hemoprotein mainly present in nerve cells called Neuroglobin was recently discovered. Enhanced expression of the protein has been shown to reduce hypoxic neural injury but the mechanism behind this function remains unknown. Methods enabling investigation of the protein in single functional neurons need to be developed. Here, we have studied how the electrical signaling capacity of a neuron was affected by hypoxic environments. Preliminary results show a trend of higher noise-level when a neuron is exposed to hypoxic compared to normoxic surroundings, which implies increased ion-channel activity. The setup used today shows shortages such as reduced control over the oxygen content due to leakage. Therefore, a gas-tight, multifunctional microfluidic system is under development which enables us to study influences of Neuroglobin concentrations on neuronal activity during hypoxia and anoxia. For electrophysiological recordings a patch-clamp micro pipette will be molded into the walls of the microfluidic system. A single biological cell is steered towards the pipette and attached there by means of optical tweezers. The Neuroglobin oxygen binding state will be studied using optical spectroscopy and the neuron environment will be manipulated by applying flows of varying oxygen content through the microfluidic system. This system will constitute a powerful tool in the investigation of the Neuroglobin mechanism of action.
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| 25. |
- Seoane, Fernando, et al.
(author)
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Electrial Bioimpedance Cerebral Monitoring
- 2008
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In: Encyclopaedia of Healthcare Information Systems. - : Encyclopaedia of Healthcare Information Systems..
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Book chapter (other academic/artistic)
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