| 1. |
- Jacobs, Kevin B, et al.
(author)
-
Detectable clonal mosaicism and its relationship to aging and cancer.
- 2012
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In: Nature Genetics. - New York : Nature Publishing Group. - 1061-4036 .- 1546-1718. ; 44:6, s. 651-658
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Journal article (peer-reviewed)abstract
- In an analysis of 31,717 cancer cases and 26,136 cancer-free controls from 13 genome-wide association studies, we observed large chromosomal abnormalities in a subset of clones in DNA obtained from blood or buccal samples. We observed mosaic abnormalities, either aneuploidy or copy-neutral loss of heterozygosity, of >2 Mb in size in autosomes of 517 individuals (0.89%), with abnormal cell proportions of between 7% and 95%. In cancer-free individuals, frequency increased with age, from 0.23% under 50 years to 1.91% between 75 and 79 years (P = 4.8 × 10(-8)). Mosaic abnormalities were more frequent in individuals with solid tumors (0.97% versus 0.74% in cancer-free individuals; odds ratio (OR) = 1.25; P = 0.016), with stronger association with cases who had DNA collected before diagnosis or treatment (OR = 1.45; P = 0.0005). Detectable mosaicism was also more common in individuals for whom DNA was collected at least 1 year before diagnosis with leukemia compared to cancer-free individuals (OR = 35.4; P = 3.8 × 10(-11)). These findings underscore the time-dependent nature of somatic events in the etiology of cancer and potentially other late-onset diseases.
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| 2. |
- Holmfeldt, Linda, et al.
(author)
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The genomic landscape of hypodiploid acute lymphoblastic leukemia
- 2013
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In: Nature Genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 45:3, s. 242-252
-
Journal article (peer-reviewed)abstract
- The genetic basis of hypodiploid acute lymphoblastic leukemia (ALL), a subtype of ALL characterized by aneuploidy and poor outcome, is unknown. Genomic profiling of 124 hypodiploid ALL cases, including whole-genome and exome sequencing of 40 cases, identified two subtypes that differ in the severity of aneuploidy, transcriptional profiles and submicroscopic genetic alterations. Near-haploid ALL with 24-31 chromosomes harbor alterations targeting receptor tyrosine kinase signaling and Ras signaling (71%) and the lymphoid transcription factor gene IKZF3 (encoding AIOLOS; 13%). In contrast, low-hypodiploid ALL with 32-39 chromosomes are characterized by alterations in TP53 (91.2%) that are commonly present in nontumor cells, IKZF2 (encoding HELIOS; 53%) and RB1 (41%). Both near-haploid and low-hypodiploid leukemic cells show activation of Ras-signaling and phosphoinositide 3-kinase (PI3K)-signaling pathways and are sensitive to PI3K inhibitors, indicating that these drugs should be explored as a new therapeutic strategy for this aggressive form of leukemia.
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| 3. |
- Wang, Zhaoming, et al.
(author)
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Imputation and subset-based association analysis across different cancer types identifies multiple independent risk loci in the TERT-CLPTM1L region on chromosome 5p15.33
- 2014
-
In: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 23:24, s. 6616-6633
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Journal article (peer-reviewed)abstract
- Genome-wide association studies (GWAS) have mapped risk alleles for at least 10 distinct cancers to a small region of 63 000 bp on chromosome 5p15.33. This region harbors the TERT and CLPTM1L genes; the former encodes the catalytic subunit of telomerase reverse transcriptase and the latter may play a role in apoptosis. To investigate further the genetic architecture of common susceptibility alleles in this region, we conducted an agnostic subset-based meta-analysis (association analysis based on subsets) across six distinct cancers in 34 248 cases and 45 036 controls. Based on sequential conditional analysis, we identified as many as six independent risk loci marked by common single-nucleotide polymorphisms: five in the TERT gene (Region 1: rs7726159, P = 2.10 × 10(-39); Region 3: rs2853677, P = 3.30 × 10(-36) and PConditional = 2.36 × 10(-8); Region 4: rs2736098, P = 3.87 × 10(-12) and PConditional = 5.19 × 10(-6), Region 5: rs13172201, P = 0.041 and PConditional = 2.04 × 10(-6); and Region 6: rs10069690, P = 7.49 × 10(-15) and PConditional = 5.35 × 10(-7)) and one in the neighboring CLPTM1L gene (Region 2: rs451360; P = 1.90 × 10(-18) and PConditional = 7.06 × 10(-16)). Between three and five cancers mapped to each independent locus with both risk-enhancing and protective effects. Allele-specific effects on DNA methylation were seen for a subset of risk loci, indicating that methylation and subsequent effects on gene expression may contribute to the biology of risk variants on 5p15.33. Our results provide strong support for extensive pleiotropy across this region of 5p15.33, to an extent not previously observed in other cancer susceptibility loci.
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| 4. |
- Andersson, Kristin, et al.
(author)
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Prospective Study of Human Papillomavirus Seropositivity and Risk of Nonmelanoma Skin Cancer
- 2012
-
In: American Journal of Epidemiology. - : Oxford University Press (OUP). - 0002-9262 .- 1476-6256. ; 175:7, s. 685-695
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Journal article (peer-reviewed)abstract
- Cutaneous human papillomaviruses (HPVs) have been associated with squamous cell carcinoma (SCC) in case-control studies, but there are limited data from prospective studies assessing whether virus exposure predicts risk of future cancer development. Two major biobanks, the Southern Sweden Microbiology Biobank (1971-2003) and the Janus Biobank (1973-2003) in Norway, containing samples from 850,000 donors, were searched for incident skin cancer for up to 30 years using registry linkages. Altogether, 2,623 donors with samples taken before diagnosis of SCC or basal cell carcinoma (BCC) of the skin were identified. Prediagnostic samples and samples from 2,623 matched controls were tested for antibodies against 33 types of HPV. Baseline seropositivity to HPV types in genus beta species 2 was associated with SCC risk (odds ratio = 1.3, 95% confidence interval: 1.1, 1.7); this was also the case for samples taken more than 18 years before diagnosis (odds ratio = 1.8, 95% confidence interval: 1.1, 2.8). Type-specific persistent seropositivity entailed elevated point estimates for SCC risk for 29 HPV types and decreased point estimates for only 3 types. After multiple hypothesis adjustment, HPV 76 was significantly associated with SCC risk and HPV 9 with BCC risk. In summary, seropositivity for certain HPV types was associated with an increased risk for future development of SCC and BCC.
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| 5. |
- Haggblade, Steven, et al.
(author)
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Cassava Commercialization in Southeastern Africa
- 2012
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In: Journal of Agribusiness in Developing and Emerging Economies. - : Emerald. - 2044-0839 .- 2044-0847. ; 2:1, s. 4-40
-
Journal article (peer-reviewed)abstract
- Purpose – Cassava production surged noticeably in Southeastern Africa beginning in the 1990s. The purpose of this paper is to examine the commercial responses and food security consequences of cassava production growth in the region. Design/methodology/approach – The paper incorporates a mix of quantitative analysis, based primarily on original analysis of national farm household survey data, together with key informant interviews with value chain participants in the three neighboring countries of Malawi, Mozambique and Zambia. Findings – In the cassava production zones, cassava's high productivity translates into per kilogram carbohydrate costs 60 per cent to 70 per cent of the cost of cereals such as maize and wheat, thereby opening up a range of profitable opportunities for commercialization of cassava-based foods, feeds and industrial products. Despite this potential, cassava commercialization in Southeastern Africa remains in its formative stages, with only 10 per cent to 30 per cent of production currently marketed. Unlike West Africa, where cassava commercialization has centered on marketing prepared cassava-based convenience foods, the emerging cassava markets in Southeastern Africa have centered on fresh cassava, low value-added cassava flour, and experiments in industrial processing of cassava-based starches, biofuels and feeds. Strategic investment in a set of key public goods (breeding, training in food sciences and food safety, and research on in-ground cassava storage) can help to shape this transition in ways that benefit both commercial interests and the food security of vulnerable households. Originality/value – The paper compares cassava commercialization across differing agro-climatic zones, policy environments and food staple zones.
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| 6. |
- Nielsen, Niklas, et al.
(author)
-
Target temperature management after out-of-hospital cardiac arrest-a randomized, parallel-group, assessor-blinded clinical trial-rationale and design
- 2012
-
In: American Heart Journal. - : Elsevier. - 0002-8703 .- 1097-6744. ; 163:4, s. 541-548
-
Journal article (peer-reviewed)abstract
- Background Experimental animal studies and previous randomized trials suggest an improvement in mortality and neurologic function with induced hypothermia after cardiac arrest. International guidelines advocate the use of a target temperature management of 32 degrees C to 34 degrees C for 12 to 24 hours after resuscitation from out-of-hospital cardiac arrest. A systematic review indicates that the evidence for recommending this intervention is inconclusive, and the GRADE level of evidence is low. Previous trials were small, with high risk of bias, evaluated select populations, and did not treat hyperthermia in the control groups. The optimal target temperature management strategy is not known. less thanbrgreater than less thanbrgreater thanMethods The TTM trial is an investigator-initiated, international, randomized, parallel-group, and assessor-blinded clinical trial designed to enroll at least 850 adult, unconscious patients resuscitated after out-of-hospital cardiac arrest of a presumed cardiac cause. The patients will be randomized to a target temperature management of either 33 degrees C or 36 degrees C after return of spontaneous circulation. In both groups, the intervention will last 36 hours. The primary outcome is all-cause mortality at maximal follow-up. The main secondary outcomes are the composite outcome of all-cause mortality and poor neurologic function (cerebral performance categories 3 and 4) at hospital discharge and at 180 days, cognitive status and quality of life at 180 days, assessment of safety and harm. less thanbrgreater than less thanbrgreater thanDiscussion The TTM trial will investigate potential benefit and harm of 2 target temperature strategies, both avoiding hyperthermia in a large proportion of the out-of-hospital cardiac arrest population.
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| 7. |
- Abdul Kader, Hale, et al.
(author)
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The determinants of reinsurance in the Swedish property fire insurance market during the interwar years, 1919–39
- 2010
-
In: Business History. - : Taylor & Francis Group. - 0007-6791 .- 1743-7938. ; 52:2, s. 268-284
-
Journal article (peer-reviewed)abstract
- Drawing a framework from agency theory, we use a panel data design to examine the factors motivating the level of demand for reinsurance in the rapidly developing Swedish property fire insurance market during the interwar period 1919–39. We find that as hypothesised, reinsurance enabled Swedish fire insurers to mitigate underwriting and solvency risks and thus increased their capacity to underwrite new business in uncertain economic times. This in turn helped to increase the supply of indemnity coverage for property (buildings) fire risks in the Swedish insurance market. We also find that as expected, investment earnings are inversely related to reinsurance purchases. However, contrary to what was hypothesised, reinsurance appears to be positively related to liquidity levels, suggesting that over our period of analysis, fire insurers could have been reinsuring to ‘protect’ earnings and accumulated cash reserves therefore enabling investment opportunities to be realised. Analysis of the sub-period 1919–28 further supports this contention, while our results for the economic depression years after 1929 show that reinsurance helped mitigate underwriting and insolvency risks, suggesting that the reinsurance decision of fire insurance companies could be motivated by macroeconomic factors.
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| 8. |
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| 9. |
- Andersson, Bo, et al.
(author)
-
Accentuated Factors of Handheld Computing
- 2013
-
In: Information Systems Development : Reflections, Challenges and New Directions - Reflections, Challenges and New Directions. - New York, NY : Springer New York. - 9781461449508 - 9781461449515 ; , s. 293-304
-
Conference paper (peer-reviewed)abstract
- The recent years of rapid development of mobile technologies creates opportunities for new user-groups in the mobile workforce to take advantage of in-formation systems (IS). However, to apprehend and harness these opportunities for mobile IS it is crucial to fully understand the user group and the mobile tech-nology. In this paper we deductively, from previous research on aspects on mobili-ty, synthesize a tentative analytical framework capturing factors accentuated in mobile IS design. We evaluate the framework based on criteria of completeness, distinctiveness, and simplicity. Eventually, these two steps develop the framework towards a theoretical contribution as theory for describing handheld computing from a designer’s perspective. Thirteen semi-structured interviews were made and the tentative framework was elaborated and confirmed.
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| 10. |
- Andersson, Daniel, 1979, et al.
(author)
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Plasticity Response in the Contralesional Hemisphere after Subtle Neurotrauma: Gene Expression Profiling after Partial Deafferentation of the Hippocampus
- 2013
-
In: Plos One. - : Public Library of Science (PLoS). - 1932-6203. ; 8:7
-
Journal article (peer-reviewed)abstract
- Neurotrauma or focal brain ischemia are known to trigger molecular and structural responses in the uninjured hemisphere. These responses may have implications for tissue repair processes as well as for the recovery of function. To determine whether the plasticity response in the uninjured hemisphere occurs even after a subtle trauma, we subjected mice to a partial unilateral deafferentation of the hippocampus induced by stereotactically performed entorhinal cortex lesion (ECL). The expression of selected genes was assessed by quantitative real-time PCR in the hippocampal tissue at the injured side and the contralesional side at day 4 and 14 after injury. We observed that expression of genes coding for synaptotagmin 1, ezrin, thrombospondin 4, and C1q proteins, that have all been implicated in the synapse formation, re-arrangement and plasticity, were upregulated both in the injured and the contralesional hippocampus, implying a plasticity response in the uninjured hemisphere. Several of the genes, the expression of which was altered in response to ECL, are known to be expressed in astrocytes. To test whether astrocyte activation plays a role in the observed plasticity response to ECL, we took advantage of mice deficient in two intermediate filament (nanofilament) proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP(-/-) Vim(-/-)) and exhibiting attenuated astrocyte activation and reactive gliosis. The absence of GFAP and vimentin reduced the ECL-induced upregulation of thrombospondin 4, indicating that this response to ECL depends on astrocyte activation and reactive gliosis. We conclude that even a very limited focal neurotrauma triggers a distinct response at the contralesional side, which at least to some extent depends on astrocyte activation.
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| 11. |
- Andersson, Hans, et al.
(author)
-
Inledning
- 2014
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In: Stormaktsstaden Jönköping : 1614 och framåt. - 9789185692903 ; , s. 13-17
-
Book chapter (peer-reviewed)
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| 12. |
- Andersson, Heléne, 1973, et al.
(author)
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Trauma-induced reactive gliosis is reduced after treatment with octanol and carbenoxolone
- 2011
-
In: Neurological research. - 0161-6412. ; 33:6, s. 614-624
-
Journal article (peer-reviewed)abstract
- Background: Reactive gliosis and scar formation after brain injury can inhibit the recovery process. As many glial cells utilize gap junctions for intercellular signaling, this study investigated whether two commonly used gap junction blockers, octanol and carbenoxolone, could attenuate reactive gliosis following a minor traumatic brain injury. Methods: Octanol (710 mg/kg) or carbenoxolone (90 mg/kg) was administered 30 minutes before or after a needle track injury in adult male Sprague-Dawley rats. To mark dividing cells, animals were injected with bromodeoxyuridine (BrdU; 150 mg/kg) intraperitoneally two times per day, 8 hours apart and killed 2 days later. Immunohistochemistry for BrdU and markers for reactive glial cells [glial fibrillary acidic protein (GFAP), ED1, and NG2] were investigated using immunohistochemistry and western blot techniques. Results: Two days after injury, increased cellular proliferation, activated astrocytes and microglia, and upregulation of NG2 expression were observed surrounding the injury site. Octanol and carbenoxolone administrated prior to injury significantly decreased cell proliferation by 60 and 70% respectively. The distance of GFAP immunoreactive astrocytes from the wound margin was decreased by 32 and 18% when octanol was administrated prior to or post injury respectively. Treatment with octanol also decreased the number of reactive microglia by 55% and, when administrated prior to injury, octanol reduced the distance of NG2 expression from the wound by 48%. Conclusion: The present study demonstrates that two important components of reactive gliosis, cellular activation and proliferation, can be attenuated by octanol and carbenoxolone.
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| 13. |
- Andersson, Kristin, et al.
(author)
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Prospective study of genital human papillomaviruses and nonmelanoma skin cancer.
- 2013
-
In: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 133:8, s. 1840-1845
-
Journal article (peer-reviewed)abstract
- Genital high-risk human papillomaviruses (HPVs) cause cervical cancer and are also found in a small proportion of nonmelanoma skin cancers (NMSCs). We used cancer registry linkages to follow the 856,000 serum donors included in the Southern Sweden Microbiology Biobank or the Janus Biobank in Norway, for incident skin cancers occurring up to 30 years after serum donation. Serum samples taken before diagnosis of squamous cell carcinoma (SCC) (N = 633), basal cell carcinoma (BCC) (N = 1990) or other NMSC (N = 153) and matched samples from control donors were tested for antibodies to the genital HPV types 16 and 18. Both HPV 16 and 18 were associated with increased risk for SCC [odds ratio (OR) 1.6, 95% confidence interval (CI) 1.1-2.6 and OR 1.7, 95% CI 1.1-2.5, respectively] and other NMSC (OR 2.3, 95% CI 1.0-5.2 and OR 3.5, 95% CI 1.4-8.7, respectively), but not for BCC. Tumor blocks from HPV16 or 18 seropositive cases were tested with real-time polymerase chain reaction for presence of HPV16 or 18 DNA. No HPV18 DNA was found and only four of 79 SCC cases (two of which were from the perineum/perianal area), one of 221 BCC cases and zero of five cases with other NMSC contained HPV16 DNA. In conclusion, we found prospective evidence that HPV16 and 18 antibodies associate with SCC and other NMSC risk, but not with BCC risk. As only a small proportion of seropositive subjects had evidence of the corresponding HPV DNA in the tumor, most of this excess risk is likely to be due to confounders associated with genital HPV infection.
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| 14. |
- ANDERSSON, MICHAEL, 1988, et al.
(author)
-
10 dB small-signal graphene FET amplifier
- 2012
-
In: Electronics Letters. - : Institution of Engineering and Technology (IET). - 1350-911X .- 0013-5194. ; 48:14, s. 861-863
-
Journal article (peer-reviewed)abstract
- Reported is the realisation of a graphene FET microwave amplifier operating at 1 GHz, exhibiting a small-signal power gain of 10 dB and a noise figure of 6.4 dB. The amplifier utilises a matching inductor on the gate yielding a return loss of 20 dB. The design is optimised for maximum gain and the optimum noise figure is extracted by noise modelling and predicted to be close to 1 dB for the intrinsic graphene FET at this frequency. The presented results complement existing graphene FET applications and are promising for future graphene microwave circuits.
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| 15. |
- Andersson, Mats, et al.
(author)
-
Adaptiv filtering of 4D-heart CT for image denoising and patient safety
- 2010
-
Conference paper (other academic/artistic)abstract
- The aim of this medical image science project is to increase patient safety in terms of improved image quality and reduced exposure to ionizing radiation in CT. The means to achieve these goals is to develop and evaluate an efficient adaptive filtering (denoising/image enhancement) method that fully explores true 4D image acquisition modes. Four-dimensional (4D) medical image data are captured as a time sequence of image volumes. During 4D image acquisition, a 3D image of the patient is recorded at regular time intervals. The resulting data will consequently have three spatial dimensions and one temporal dimension. Increasing the dimensionality of the data impose a major increase the computational demands. The initial linear filtering which is the cornerstone in all adaptive image enhancement algorithms increase exponentially with the dimensionality. On the other hand the potential gain in Signal to Noise Ratio (SNR) also increase exponentially with the dimensionality. This means that the same gain in noise reduction that can be attained by performing the adaptive filtering in 3D as opposed to 2D can be expected to occur once more by moving from 3D to 4D. The initial tests on on both synthetic and clinical 4D images has resulted in a significant reduction of the noise level and an increased detail compared to 2D and 3D methods. When tuning the parameters for adaptive filtering is extremely important to attain maximal diagnostic value which not necessarily coincide with an an eye pleasing image for a layman. Although this application focus on CT the resulting adaptive filtering methods will be beneficial for a wide range of 3D/4D medical imaging modalities e.g. shorter acquisition time in MRI and improved elimination of noise in 3D or 4D ultrasound datasets.
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| 16. |
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| 17. |
- Andersson, Marlene, et al.
(author)
-
Carbonic Anhydrase Generates CO2 and H+ That Drive Spider Silk Formation Via Opposite Effects on the Terminal Domains
- 2014
-
In: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 12:8, s. e1001921-
-
Journal article (peer-reviewed)abstract
- Spider silk fibers are produced from soluble proteins (spidroins) under ambient conditions in a complex but poorly understood process. Spidroins are highly repetitive in sequence but capped by nonrepetitive N- and C-terminal domains (NT and CT) that are suggested to regulate fiber conversion in similar manners. By using ion selective microelectrodes we found that the pH gradient in the silk gland is much broader than previously known. Surprisingly, the terminal domains respond in opposite ways when pH is decreased from 7 to 5: Urea denaturation and temperature stability assays show that NT dimers get significantly stabilized and then lock the spidroins into multimers, whereas CT on the other hand is destabilized and unfolds into ThT-positive beta-sheet amyloid fibrils, which can trigger fiber formation. There is a high carbon dioxide pressure (pCO(2)) in distal parts of the gland, and a CO2 analogue interacts with buried regions in CT as determined by nuclear magnetic resonance (NMR) spectroscopy. Activity staining of histological sections and inhibition experiments reveal that the pH gradient is created by carbonic anhydrase. Carbonic anhydrase activity emerges in the same region of the gland as the opposite effects on NT and CT stability occur. These synchronous events suggest a novel CO2 and proton-dependent lock and trigger mechanism of spider silk formation.
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| 18. |
- Andersson, Mattias
(author)
-
Coding for the Wiretap Channel
- 2011
-
Licentiate thesis (other academic/artistic)abstract
- We consider code design for Wyner’s wiretap channel. Optimal coding schemes for this channel require an overall code that is capacity achieving for the main channel, partitioned into smaller subcodes, all of which are capacity achieving for the wiretapper’s channel. To accomplish this we introduce two edge type low density parity check (LDPC) ensembles for the wiretap channel. For the scenario when the main channel is error free and the wiretapper’s channel is a binary erasure channel (BEC) we find secrecy capacity achieving code sequences based on standard LDPC code sequences for the BEC. However, this construction does not work when there are also erasures on the main channel. For this case we develop a method based on linear programming to optimize two edge type degree distributions. Using this method we find code ensembles that perform close to the secrecy capacity of the binary erasure wiretap channel (BEC- WT). We generalize a method of M ́easson, Montanari, and Urbanke in order to compute the conditional entropy of the message at the wire- tapper. This conditional entropy is a measure of how much information is leaked to the wiretapper. We apply this method to relatively simple ensembles and find that they show very good secrecy performance. Based on the work of Kudekar, Richardson, and Urbanke, which showed that regular spatially coupled codes are capacity achieving for the BEC, we construct a regular two edge type spatially coupled ensem- ble. We show that this ensemble achieves the whole capacity-equivocation region for the BEC-WT. We also find a coding scheme using Arıkans polar codes. These codes achieve the whole capacity-equivocation region for any symmetric binary input wiretap channel where the wiretapper’s channel is degraded with respect to the main channel.
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| 19. |
- ANDERSSON, MICHAEL, 1988, et al.
(author)
-
Comparison of carrier scattering mechanisms in chemical vapor deposited graphene on fused silica and strontium titanite substrates
- 2014
-
In: Graphene Week 2014.
-
Conference paper (peer-reviewed)abstract
- Graphene is explored for numerous applications for both electronics and photonics. These range from high frequency and low noise field effect transistors to conductive and highly transparent LED electrodes. To exploit the full potential of graphene, the remarkable intrinsic carrier transport properties and tunable, potentially low sheet resistance must be efficiently utilized. However, graphene carrier mobility is currently strongly degraded by extrinsic factors arising mainly from the dielectric environment, i.e. substrate and gate oxide. A proposed route to enhance transport is the use of a high-κ substrate to screen charged impurities at the graphene-substrate interface. In this paper, mobility and carrier concentration in CVD grown graphene films on fused silica (FS, κ=3.9) and strontium titanite (STO, κ=300) substrates are extracted from microwave measurements and compared to Hall data. To model the mobilities scattering by charged impurities (CI), substrate polar phonons (SPP) and resonant centers (RS) are included. Resonant scatterers dominates on strontium titanite and together with charged impurities on fused silica. While resonant scatterers are likely reduced by moving from wet to dry graphene transfer methods, the nominal mobility improvement by screening of charged impurities on high- κ strontium titanite would be masked at room temperature by increased surface phonon scattering.
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| 20. |
- ANDERSSON, MICHAEL, 1988, et al.
(author)
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Extraction of carrier transport properties in graphene from microwave measurements
- 2014
-
In: European Microwave Conference (EuMC), 2014 44th. ; , s. 359 - 362
-
Conference paper (peer-reviewed)abstract
- Carrier transport parameters of graphene grown by chemical vapor deposition (CVD) and graphene-metal contacts are extracted from microwave measurements in the frequency range 0.1–20 GHz using Corbino disks. It is shown that the charged impurities are effectively screened by the high permittivity of the SrTiO3 substrate. In the case of fused silica substrate the charged impurities are not completely screened and the mobility is limited either by the charged impurities or/and resonant scatterers depending on their relative concentration.
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| 21. |
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| 22. |
- Andersson, Mattias K., et al.
(author)
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Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2′ of substrates
- 2010
-
In: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 277:10, s. 2255-2267
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Journal article (peer-reviewed)abstract
- Chymases are chymotrypsin-like serine proteases that are found in large amounts in mast cell granules. So far, the extended cleavage specificities of eight such chymases have been determined, and four of these were shown to have a strong preference for acidic amino acids at position P2'. These enzymes have basic amino acids in positions 143 and 192 (Arg and Lys, respectively). We therefore hypothesized that Arg143 and Lys192 of human chymase mediate the preference for acidic amino acids at position P2' of substrates. In order to address this question, we performed site-directed mutagenesis of these two positions in human chymase. Analysis of the extended cleavage specificities of two single mutants (Arg143 -> Gln and Lys192 -> Met) and the combined double mutant revealed an altered specificity for P2' amino acids, whereas all other positions were essentially unaffected. A weakened preference for acidic amino acids at position P2' was observed for the two single mutants, whereas the double mutant lacked this preference. Therefore, we conclude that positions 143 and 192 in human chymase contribute to the strong preference for negatively charged amino acids at position P2'. This is the first time that a similar combined effect has been shown to influence the cleavage specificity, apart from position P1, among the chymases. Furthermore, the conservation of the preference for acidic P2' amino acids for several mast cell chymases clearly indicates that other substrates than angiotensin I may be major in vivo targets for these enzymes.
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| 23. |
- ANDERSSON, MICHAEL, 1988
(author)
-
Microwave characterisation of electrodes and field effect transistors based on graphene
- 2014
-
Licentiate thesis (other academic/artistic)abstract
- The isolation of the two-dimensional material graphene, a single hexagonal sheet of carbon atoms, is believed to trigger a revolution in electronics. Theory predicts unprecedented carrier velocities in ideal graphene, from which ultrahigh speed graphene field effect transistors (GFETs) are envisioned. In this thesis, the prospects of GFETs for microwave receivers are investigated with the emphasis on low noise amplifiers (LNAs). A microwave amplifier at 1 GHz with 10 dB small-signal gain and 6.4 dB noise figure was realised using a mechanically exfoliated graphene flake on a SiO2 substrate. Comparable GFET performance was demonstrated with large-area graphene grown by chemical vapour deposition (CVD) on copper and transferred to SiO2. From a device level noise characterisation, the CVD GFET minimum noise figure (Fmin) in the frequency range 2-8 GHz was measured to be 2.5-5 dB and estimated by de-embedding parasitics to be 1-4 dB for the intrinsic device. However, the GFET noise is sensitive to impedance mismatch as the noise resistance is high. In addition, subharmonic resistive GFET mixers utilising the symmetry of electron and hole conduction in graphene were assessed. Conversion loss (CL) and noise figure were approximately equal and ≥20 dB and the input third order intercept point (IIP3) was ≤3.9 dBm at a local oscillator power of 2 dBm, less linear then fundamental resistive mixers. Finally, the properties of graphene and metal-graphene contacts were investigated by parameter extraction based on measurements at both DC and microwave frequencies. Using a palladium based contact, a contact resistance as low as
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| 24. |
- ANDERSSON, MICHAEL, 1988, et al.
(author)
-
Microwave characterization of Ti/Au-graphene contacts
- 2013
-
In: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 103:17, s. 173111-
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Journal article (peer-reviewed)abstract
- In this paper, we report on a microwave characterization of the interface between Ti/Au contacts and chemical vapor deposition graphene using structures of Corbino geometry, with primary focus on extracting and modeling the capacitance associated with the contact region. It is found that with the current contact resistivity, ρc∼10^−6 Ωcm2, the contact capacitance, on the order Cc∼1 μF/cm2, has a negligible effect on microwave transmission through the contact below ∼100 GHz. Finally, a parallel plate capacitance model for the contact is presented.
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| 25. |
- Andersson, Michael, 1980, et al.
(author)
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Muscarinic receptor subtypes involved in urothelium-derived relaxatory effects in the inflamed rat urinary bladder.
- 2012
-
In: Autonomic neuroscience : basic & clinical. - : Elsevier BV. - 1872-7484. ; 170:1-2, s. 5-11
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Journal article (peer-reviewed)abstract
- Functional studies have shown altered cholinergic mechanisms in the inflamed bladder, which partly depend on muscarinic receptor-induced release of nitric oxide (NO). The current study aimed to characterize which muscarinic receptor subtypes that are involved in the regulation of the nitrergic effects in the bladder cholinergic response during cystitis. For this purpose, in vitro examinations of carbachol-evoked contractions of inflamed and normal bladder preparations were performed. The effects of antagonists with different selectivity for the receptor subtypes were assessed on intact and urothelium-denuded bladder preparations. In preparations from cyclophosphamide (CYP; in order to induce cystitis) pre-treated rats, the response to carbachol was about 75% of that of normal preparations. Removal of the urothelium or administration of a nitric oxide synthase inhibitor re-established the responses in the inflamed preparations. Administration of 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) inhibited the carbachol-induced contractile responses of preparations from CYP pre-treated rats less potently than controls. Pirenzepine and p-fluoro-hexahydro-sila-diphenidol (pFHHSiD) affected the carbachol-induced contractile responses to similar extents in preparations of CYP pre-treated and control rats. However, the Schild slopes for the three antagonists were all significantly different from unity in the preparations from CYP pre-treated rats. Again, l-NNA or removal of the urothelium eliminated any difference compared to normal preparations. This study confirms that muscarinic receptor stimulation in the inflamed rat urinary bladder induces urothelial release of NO, which counteracts detrusor contraction.
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