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Träfflista för sökning "WFRF:(Chen Lili) srt2:(2006-2009)"

Search: WFRF:(Chen Lili) > (2006-2009)

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1.
  • Sodergren, Erica, et al. (author)
  • The genome of the sea urchin Strongylocentrotus purpuratus.
  • 2006
  • In: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 314:5801, s. 941-52
  • Journal article (peer-reviewed)abstract
    • We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes.
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2.
  • Li, Xin, et al. (author)
  • Nanoparticle-assisted DNA nanosensor
  • 2008
  • In: AOE 2007. - SHENZHEN : AOE. - 9780978921736 ; , s. 84-86
  • Conference paper (peer-reviewed)abstract
    • We report a sensitive nanosensor based on a micro-fluidic chip and nanoparticles to detect low concentrations of DNA. The emission of CdSe/ZnS-QDs linked with single strand DNAs was quenched by gold nanoparticles linked with the complementary sequences after hybridization. Sensitively detected signal of DNA was obtained from a 100 mu m capillary.
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3.
  • Schnorrer, Frank, et al. (author)
  • Positional cloning by fast-track SNP-mapping in Drosophila melanogaster
  • 2008
  • In: Nature Protocols. - : Springer Science and Business Media LLC. - 1754-2189 .- 1750-2799. ; 3:11, s. 1751-1765
  • Journal article (peer-reviewed)abstract
    • Positional cloning of chemically induced mutations is the rate-limiting step in forward genetic screens in Drosophila. Single-nucleotide polymorphisms (SNPs) are useful markers to locate a mutated region in the genome. Here, we provide a protocol for high-throughput, high-resolution SNP mapping that enables rapid and cost-effective positional cloning in Drosophila. In stage 1 of the protocol, we use highly multiplexed tag-array mini-sequencing assays to map mutations to an interval of 1-2 Mb. In these assays, SNPs are genotyped by primer extension using fluorescently labeled dideoxy-nucleotides. Fluorescent primers are captured and detected on a microarray. In stage 2, we selectively isolate recombinants within the identified 1-2 Mb interval for fine mapping of mutations to about 50 kb. We have previously demonstrated the applicability of this protocol by mapping 14 muscle morphogenesis mutants within 4 months, which represents a significant acceleration compared with other commonly used mapping strategies that may take years.
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  • Result 1-3 of 3

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