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- Eklöw, Annelie, et al.
(author)
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Three important clones of Listeria monocytogenes
- 1996
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In: Proceedings of the symposium Food Associated Pathogens, May 6-8, 1996, Uppsala, Sweden. - Uppsala : SLU, Department of Food Hygiene. - 9157651329 ; , s. 221-222
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Conference paper (peer-reviewed)abstract
- The object of this study was to characterize Swedish human strains of Listeria monocytogenes
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- Ericsson, Henrik, et al.
(author)
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An outbreak of listeriosis suspected to have been caused by rainbow trout
- 1997
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In: Journal of Clinical Microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 35:11, s. 2904-2907
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Journal article (peer-reviewed)abstract
- An outbreak of listeriosis in Sweden, consisting of nine cases, was investigated by means of molecular typing of strains from patients and strains isolated from suspected foodstuffs, together with interviews of the patients. Listeria monocytogenes was isolated from six of the patients, and all isolates were of the same clonal type. This clonal type was also isolated from a 'gravad' rainbow trout, made by producer Y, found in the refrigerator of one of the patients. Unopened packages obtained from producer y were also found to contain the same clonal type of L. monocytogenes. Based on the interview results and the bacteriological typing, we suspect that at least six of the nine cases were caused by gravad or cold-smoked rainbow trout made by producer Y. To our knowledge, this is the first rainbow trout-borne outbreak of listeriosis ever reported.
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- Ericsson, Henrik, et al.
(author)
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Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis
- 1995
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In: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 61:11, s. 3872-3874
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Journal article (peer-reviewed)abstract
- Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated, A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively, The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.
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- Tham, Wilhelm, 1951-, et al.
(author)
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Listeria monocytogenes subtypes associated with mortality among fallow deer (Dama dama)
- 1999
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In: Journal of zoo and wildlife medicine. - 1042-7260 .- 1937-2825. ; 30:4, s. 545-549
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Journal article (peer-reviewed)abstract
- Different subtypes of Listeria monocytogenes were isolated from various animal and environmental samples during an episode of increased mortality on a fallow deer (Dama dama) farm. During a 4-wk period, six fallow deer died, including four does, one fawn, and one adult buck. Prior to death, one of the does had exhibited central nervous system signs characteristic of listeriosis. Postmortem examination of the six deer showed no histologic changes typical of listeriosis, although inflammatory changes were present in several organs. Different subtypes of L. monocytogenes were isolated from brain samples from six deer, from fodder and soil from the deer feeding area, and from faces of some healthy animals on the farm. Listeria monocytogenes, which was frequently isolated in the environment of the farm, was considered the probable major cause of mortality in these fallow deer.
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- Unnerstad, Helle, et al.
(author)
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Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis
- 1999
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In: Applied and Environmental Microbiology. - Washington DC, USA : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 65:5, s. 2054-2056
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Journal article (peer-reviewed)abstract
- Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), and 1,876 bp of the gene inlB, was cleaved with the enzyme AluI, and the fragments generated were separated by gel electrophoresis. By this method two different cleavage patterns were obtained. Seventy of the 100 strains shared one restriction profile, and the remaining 30 strains shared the second one. No relation was found between the types differentiated by PCR-REA and the origins of the strains.
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