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- Hayes, A., et al.
(author)
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A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts
- 2020
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In: Journal of Medical Virology. - : Wiley. - 0146-6615 .- 1096-9071. ; 92:8, s. 1065-1074
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Journal article (peer-reviewed)abstract
- Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 µL) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 µL) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 × 10−5). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests.
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| 2. |
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| 3. |
- Stefan, Ioana, et al.
(author)
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The dark side of open innovation : Individual affective responses as hidden tolls of the paradox of openness
- 2022
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In: Journal of Business Research. - : Elsevier Inc.. - 0148-2963 .- 1873-7978. ; 138, s. 360-373
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Journal article (peer-reviewed)abstract
- While creating value in open innovation (OI) requires knowledge sharing, appropriating value in OI entails some closedness and protectiveness. Hence, tensions between generating and appropriating value, known as the paradox of openness, may emerge in OI collaborations. Such tensions have been scarcely explored at the micro-level, even though it is a crucial piece to fully grasping the paradox of openness. Our study bridges this gap by examining individuals’ affective responses to tensions and their outcomes in OI, thereby capturing the microfoundations of the paradox of openness. The study adopts an inductive qualitative approach and delineates various micro-level coping mechanisms that build on figurative language and humor. Accordingly, our study reveals hidden tolls of the paradox of openness, highlighted by the dominance of destructive rather than constructive affective responses. These hidden tolls illuminate a “dark side” of OI, which taps into the potential failures and high costs of opening up.
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