| 1. |
- Johnsson, Anna-Ida, et al.
(author)
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Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples
- 2011
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In: BioControl. - : Springer Science and Business Media LLC. - 1386-6141 .- 1573-8248. ; 56, s. 903-913
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Journal article (peer-reviewed)abstract
- Several strains from the genus Trichoderma (Ascomycetes, Hypocreales) are commercially used as biocontrol agents, e.g. in formulations containing the two Trichoderma strains IMI206039 (Hypocrea parapilulifera B.S. Lu, Druzhinina & Samuels) and IMI206040 (T. atroviride P. Karst). To quantify the presence of the two isolates after application, we developed primers for SCAR markers (Sequence-Characterised Amplified Region). In order to quantify both fungal strains simultaneously, we also designed fluorophore-labelled probes distinguishing the two strains, to be used in combination with the SCAR primers. In incubations of two different soils, artificially inoculated and maintained under controlled conditions, the quantification through amplification with the SCAR markers in qPCR and through colony-forming units from plate counting correlated well. Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish, either on the golf green or in the surrounding area.
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| 2. |
- Von Rosen, Dietrich
(author)
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Non-negative estimation of variance components in heteroscedastic one-way random-effects ANOVA models
- 2010
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In: Statistics. - : Informa UK Limited. - 0233-1888 .- 1029-4910. ; 44, s. 557-569
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Journal article (peer-reviewed)abstract
- There is a considerable amount of literature dealing with inference about the parameters in a heteroscedastic one-way random-effects ANOVA model. In this paper, we primarily address the problem of improved quadratic estimation of the random-effect variance component. It turns out that such estimators with a smaller mean squared error compared with some standard unbiased quadratic estimators exist under quite general conditions. Improved estimators of the error variance components are also established.
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| 3. |
- Skovsgaard, Jens Peter
(author)
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Bog om tarmvridrøn
- 2013
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In: Skoven. - 0106-8539. ; 45, s. 551-551
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Other publication (other academic/artistic)abstract
- Book review N. Mayer & R.T. Klumpp (ed.) 2013: Elsbeere in Österreich – Monographie. Verein zur Erhaltung, Pflege und Vermarktung der Elsbeere - Genussregion Wiesenwienerwald Elsbeere, Michelbach.
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| 4. |
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| 5. |
- Messing, Ingmar
(author)
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Land and land use : Natural preconditions
- 2011
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In: Agriculture and Forestry in Sweden since 1900- a cartographic description. - 9789187760617 ; , s. 14-22
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Book chapter (other academic/artistic)
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| 6. |
- Messing, Ingmar
(author)
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Marken i Sverige : Naturliga förutsättningar
- 2011
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In: Sveriges nationalatlas. Jordbruk och skogsbruk i Sverige sedan år 1900: en kartografisk beskrivning. - 9789187760594 ; , s. 14-22
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Book chapter (other academic/artistic)
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| 7. |
- Thulin, Carl-Gustaf
(author)
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Utsättning av arter : möjlighet för bevarandebiologin
- 2010
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In: Biodiverse. - 1401-5064. ; 15, s. 8-9
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Journal article (pop. science, debate, etc.)abstract
- Ett halvöppet, lövträdsdominerat landskap med betande hjortdjur, visenter, förvildad nötboskap och vildsvin, jagade av livskraftiga populationer av varg, björn och lo. Utopier och vilda fantasier? Kanske i delar, men något håller definitivt på att hända med bevarandet, återskapandet och skötseln av de europeiska vildmarkerna
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| 8. |
- Tunon, Håkan, et al.
(author)
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Kulturarv och hållbar utveckling
- 2010
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In: Bebyggelsehistorisk tidskrift. - : Föreningen Bebyggelsehistorisk tidskrift. - 0349-2834 .- 2002-3812. ; , s. 99–106-
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Journal article (other academic/artistic)
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| 9. |
- Ritter, Camila, et al.
(author)
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Biodiversity assessments in the 21st century: The potential of insect traps to complement environmental samples for estimating eukaryotic and prokaryotic diversity using high-throughput DNA metabarcoding.
- 2019
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In: Genome. - : Canadian Science Publishing. - 1480-3321 .- 0831-2796. ; 62:3, s. 147-159
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Journal article (peer-reviewed)abstract
- The rapid loss of biodiversity, coupled with difficulties in species identification, call for innovative approaches to assess biodiversity. Insects make up a substantial proportion of extant diversity and play fundamental roles in any given ecosystem. To complement morphological species identification, new techniques such as metabarcoding make it possible to quantify insect diversity and insect-ecosystem interactions through DNA sequencing. Here we examine the potential of bulk insect samples (i.e., containing many non-sorted specimens) to assess prokaryote and eukaryote biodiversity and to complement the taxonomic coverage of soil samples. We sampled 25 sites on three continents and in various ecosystems, collecting insects with Slam-traps (Brazil) and Malaise-traps (South Africa and Sweden). We then compared our diversity estimates with the results obtained with biodiversity data from soil samples from the same localities. We found a largely different taxonomic composition between the soil and insect samples, testifying to the potential of bulk insect samples to complement soil samples. Finally, we found that non-destructive DNA extraction protocols, which preserve insect specimens for morphological studies, constitute a promising choice for cost-effective biodiversity assessments. We propose that the sampling and sequencing of insect samples should become a standard complement for biodiversity studies based on environmental DNA.
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| 10. |
- Mattsson, Eskil, 1981, et al.
(author)
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Quantification of carbon stock and tree diversity of homegardens in a dry zone area of Moneragala District, Sri Lanka
- 2015
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In: Agroforestry Systems. - : Springer Science and Business Media LLC. - 1572-9680 .- 0167-4366. ; 89:3, s. 435-445
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Journal article (peer-reviewed)abstract
- Homegarden agroforestry systems are suggested to hold a large potential for climate change mitigation and adaptation. This is due to their multifunctional role in providing income, food and ecosystem services while decreasing pressure on natural forests and hence saving and storing carbon. In this paper, above-ground biomass carbon and tree species diversity of trees was quantified in homegardens around two villages in the dry south-eastern part of Moneragala district of Sri Lanka. A total of 45 dry zone homegardens were sampled on size, diameter at breast height, tree height and species diversity. Using allometric equations, we find a mean above-ground biomass stock of 13 mega grams of carbon per hectare (Mg C ha−1) with a large range among homegardens (1–56 Mg C ha−1, n = 45) due to a variation of tree diversity and composition between individual homegardens. Mean above-ground carbon stock per unit area was higher in small homegardens (0.2 ha, 26 Mg C ha−1, n = 11) and statistically different compared to medium (0.4–0.8 ha, 9 Mg C ha−1, n = 27) and large (1.0–1.2 ha, 8 Mg C ha−1, n = 7) homegardens. In total, 4,278 trees were sampled and 70 tree species identified and recorded. The Shannon Wiener index were used to evaluate diversity per homegarden and ranged from 0.76 to 3.01 with a mean value of 2.05 ± 0.07 indicating a medium evenly distributed diversity of sampled tree species. The results show a vast heterogeneity in terms of carbon stock and tree diversity within the less studied dry zone homegardens; results that contribute to more knowledge of their expansion potential as well as climate mitigation and adaptation potential. The results are also useful for whether homegardens should be considered to be included as an activity to enhance natural forest cover within Sri Lanka’s newly commenced UN-REDD National Programme.
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| 11. |
- Norman, Johan
(author)
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Skogen – en plats för upplevelser
- 2011
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In: Grön Entreprenör, Naturupplevelse och hälsa – forskningen visar vägen. - 9789157690364 ; , s. 62-75
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Book chapter (pop. science, debate, etc.)
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| 12. |
- Palsdottir, Anna Maria, et al.
(author)
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Nature-Based Therapeutic Interventions
- 2011
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In: Forests, Trees and Human Health. - Dordrecht : Springer Netherlands. - 9789048198054 ; , s. 309-342
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Book chapter (other academic/artistic)
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| 13. |
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Unraveling the logics of landscape
- 2014
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Editorial collection (other academic/artistic)abstract
- Despite conceptual oscillations through times, the concept of landscape remains highly subjective, whereupon unraveling its 'logics' opens up to a plurality of interpretations. Accordingly, by focusing on the interconnections present in the non-haphazard production of landscape, this publication elaborates on how the rural landscape is valued, monitored, changed, harbored, used and misused, be it through actions, representations or metaphors. This book covers a broad range of topics, with contributions from scholars from more than 30 countries.
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| 14. |
- Abarenkov, Kessy, et al.
(author)
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Protax-fungi: A web-based tool for probabilistic taxonomic placement of fungal internal transcribed spacer sequences
- 2018
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In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 220:2, s. 517-525
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Journal article (peer-reviewed)abstract
- © 2018 New Phytologist Trust. Incompleteness of reference sequence databases and unresolved taxonomic relationships complicates taxonomic placement of fungal sequences. We developed Protax-fungi, a general tool for taxonomic placement of fungal internal transcribed spacer (ITS) sequences, and implemented it into the PlutoF platform of the UNITE database for molecular identification of fungi. With empirical data on root- and wood-associated fungi, Protax-fungi reliably identified (with at least 90% identification probability) the majority of sequences to the order level but only around one-fifth of them to the species level, reflecting the current limited coverage of the databases. Protax-fungi outperformed the Sintax and Rdb classifiers in terms of increased accuracy and decreased calibration error when applied to data on mock communities representing species groups with poor sequence database coverage. We applied Protax-fungi to examine the internal consistencies of the Index Fungorum and UNITE databases. This revealed inconsistencies in the taxonomy database as well as mislabelling and sequence quality problems in the reference database. The according improvements were implemented in both databases. Protax-fungi provides a robust tool for performing statistically reliable identifications of fungi in spite of the incompleteness of extant reference sequence databases and unresolved taxonomic relationships.
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| 15. |
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| 16. |
- Erhagen, Björn, et al.
(author)
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Temperature sensitivity of heterotrophic soil CO2 production increases with increasing carbon substrate uptake rate
- 2015
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In: Soil Biology and Biochemistry. - : Elsevier BV. - 0038-0717 .- 1879-3428. ; 80, s. 45-52
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Journal article (peer-reviewed)abstract
- Temperature profoundly affects saprotrophic respiration rates, and carbon quality theory predicts that the rates' temperature Sensitivity should increase as the quality of the carbon source declines. However, reported relationships between saprotrophic respiration responses to temperature and carbon quality vary widely. Some of this variability may arise from confounding effects related to both substrate quality and substrate availability. The importance of these variables, as well as substrate diffusion and uptake rates, for the temperature sensitivity of saprotrophic respiration has been validated theoretically, but not empirically demonstrated. Thus, we tested effects of varying substrate uptake rates on the temperature sensitivity of organic carbon degradation. For this purpose we created a model system using the organic layer (O-horizon), of a boreal forest soil, specifically to test effects of varying monomer uptake and release rates. The addition of both monomers and polymers generally increased the temperature sensitivity of saprotrophic respiration. In response to added monomers, there was a linear increase in the temperature sensitivity of both substrate-induced respiration and the specific growth rate with increasing rate of substrate uptake as indicated by the CO2 production at 14 degrees C. Both of these responses diverge from those predicted by the carbon quality theory, but they provide the first empirical evidence consistent with model predictions demonstrating increased temperature sensitivity with increased uptake rate of carbon monomers over the cell membrane. These results may explain why organic material of higher carbon quality induces higher temperature responses than lower carbon quality compounds, without contradicting carbon quality theory.
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| 17. |
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| 18. |
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| 19. |
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| 20. |
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| 21. |
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| 22. |
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| 23. |
- Fernando, Dinesh, et al.
(author)
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Characterization and biological depectinization of hemp fibers originating from different stem sections
- 2015
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In: Industrial Crops and Products. - : Elsevier BV. - 0926-6690 .- 1872-633X. ; 76, s. 880-891
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Journal article (peer-reviewed)abstract
- The wide variation of mechanical properties of natural fibers limits their applications in matrix compos-ites. The aim of this study is to evaluate the properties of hemp fibers from different stem sections (top,middle and bottom) and to assess fungal retting pretreatment of hemp from different stem sections withthe white rot fungi Phlebia radiata Cel 26 and Ceriporiopsis subvermispora. For the untreated hemp fibers,no apparent difference in tensile behavior for fiber bundles from different stem sections was observed,and more than 90% tested samples demonstrated plastic flow behavior. Fiber strength and stiffness werehighest for the fibers from the top and middle stem sections. These properties were related to the compositional make up and morphological properties of hemp fibers, notably the secondary fiber cell contents.In fungal retting, there was a strong dependence of depectinization selectivity on stem section, whichdecreased from bottom to top presumably due to the significantly higher lignin content in the bottomsection than in the top section (middle section was in between). Consequently, the fungal retting caused alower reduction in strength of fibers from the bottom section than in those from the top stem section, andessentially reversed the influence of stem section on fiber tensile strength through depectinization selec-tivity. At whole hemp stem level, the fungal retting with P. radiata Cel 26 exhibited better mechanicalproperties with an ultimate tensile strength, strain and stiffness of 736 MPa, 2.3% and 42 GPa, respec-tively, while fibers treated with C. subvermispora exhibited lower mechanical properties of 573 MPa, 1.9% and 40 GPa, respectively. The study thus also showed that less variable and high strength fibers may beproduced using the dependence of depectinization selectivity on stem section for composite application
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| 24. |
- Fernando, Dinesh, et al.
(author)
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Effect of harvest time and field retting duration on the chemical composition, morphology and mechanical properties of hemp fibers
- 2015
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In: Industrial Crops and Products. - : Elsevier BV. - 0926-6690 .- 1872-633X. ; 69, s. 29-39
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Journal article (peer-reviewed)abstract
- The large variability in the mechanical properties of hemp fibers creates difficulties for using them in high-grade composites. The objective of the present study was to determine the optimal growth stage for harvesting hemp fibers for use in composites and to evaluate the effect of field retting time on mechanical performance of the fibers. Reduction in bast content and thickness of the primary bast fiber layer in stems were found to be highly significant (P ˂ 0.01) with plant maturity. A significant increase in the secondary fiber fraction occurred with maturity, reaching a maximum value of 10% at seed maturity. A highly significant reduction in cellulose deposition in fiber cell walls was reflected by reduced fiber wall thickness with plant maturity and was related to the development and ripening of hemp seeds. A statistically significant increase in lignin deposition and slight decrease in pectins in hemp fiber cell walls was also noted with stem maturity. Microscopy observations and histochemical analyses corroborated results from chemical analyses and showed variations in morphological aspects and spatial micro-distributions of carbohydrates and lignin within the cell structure of the hemp stems between early- and late growth phases. Fibers harvested at the beginning of flowering exhibited high tensile strength and strain, which decreased with plant maturity. Reduction in strength was related to the increase in proportion of secondary fibers and decrease in cellulose deposition leading to inferior properties of the primary fibers. A negative effect of field retting occurred only after long term field retting (i.e. 70 days) which was presumably due to accelerated degradation of cellulose by the action of microorganisms.
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| 25. |
- Fransson, Petra, et al.
(author)
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Mycorrhizal associations and soil properties of native Allanblackia stuhlmannii stands in the Eastern Usambara Mountains, Tanzania
- 2016
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In: Annals of Applied Biology. - : Wiley. - 0003-4746. ; 169, s. 369-383
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Journal article (peer-reviewed)abstract
- Allanblackia stuhlmannii is a tree species currently under domestication. Potential mycorrhizal relationships of A. stuhlmannii and soil properties of native stands were investigated to determine the soil-environmental requirements of the species. Roots and soil samples were collected from five sites with A. stuhlmannii stands along an altitudinal transect in Amani Nature Reserve, Tanzania. Mycorrhizal status was investigated by combining microscopy with molecular analysis of the fungal communities. Soil adjacent to the A. stuhlmannii seedlings was analysed for physical and chemical properties and the sites were characterised. We showed that A. stuhlmannii form symbiosis with arbuscular mycorrhizal fungi, and that there was a diverse microbiome associated with the roots. The soils, classified as Ferralsol and Acrisol, were very well drained, had a pH(CaCl2) generally at or below 4, high exchangeable acidity and content of sesquioxides and low effective cation exchange capacity and concentrations of most nutrients. We conclude that A. stuhlmannii is tolerant to high Al availability and possesses mechanisms for acquisition of P and other macronutrients at low soil availability, possibly through mycorrhizal symbiosis. However, being adapted to low-pH soils, it may be less efficient in acquiring Fe, Mn and/or Zn at higher soil pH. Thus, it may be most suited to introduction on farms situated on acid soils.
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