| 1. |
- Fu, Baifeng, et al.
(author)
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Three novel umami peptides derived from the alcohol extract of the Pacific oyster (Crassostrea gigas) : identification, characterizations and interactions with T1R1/T1R3 taste receptors
- 2024
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In: FOOD SCIENCE AND HUMAN WELLNESS. - : Tsinghua University Press. - 2213-4530. ; 13:1, s. 146-153
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Journal article (peer-reviewed)abstract
- Oyster (Crassostrea gigas), the main ingredient of oyster sauce, has a strong umami taste. In this study, three potential umami peptides, FLNQDEEAR (FR-9), FNKEE (FE-5), and EEFLK (EK-5), were identified and screened from the alcoholic extracts of the oyster using nano-HPLC-MS/MS analysis, iUmami-Scoring Card Method (iUmami-SCM) database and molecular docking (MD). Sensory evaluation and electronic tongue analysis were further used to confirm their tastes. The threshold of the three peptides ranged from 0.38 to 0.55 mg/mL. MD with umami receptors T1R1/T1R3 indicated that the electrostatic interaction and hydrogen bond interaction were the main forces involved. Besides, the Phe592 and Gln853 of T1R3 were the primary docking site for MD and played an important role in umami intensity. Peptides with two Glu residues at the terminus had stronger umami, especially at the C-terminus. These results contribute to the understanding of umami peptides in oysters and the interaction mechanism between umami peptides and umami receptors. & COPY; 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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| 2. |
- Yuan, Zhen, et al.
(author)
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Immunomodulatory effect of ethanol-soluble polypeptides from Atlantic cod (Gadus morhua)
- 2023
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In: FOOD SCIENCE AND HUMAN WELLNESS. - : Elsevier. - 2213-4530. ; 12:4, s. 1192-1203
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Journal article (peer-reviewed)abstract
- There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80% ethanol extraction method, the isolated ethanol-soluble cod peptides (CP-ES) were investigated and their immunomodulatory activity was verified. Additionally, CP-ES showed lower molecular weight and more hydrophobic amino acids. CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice, suggesting that CP-ES may regulate adaptive immunity. It promoted the release of NO and the expression of iNOS, TNF-alpha, IL-6 and IL-1 beta genes in macrophages, suggesting that CP-ES may regulate innate immunity. CP-ES could promote the expression of TLR2 gene, and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES. These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines.
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| 3. |
- Zhu, Dongyang, et al.
(author)
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The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates
- 2023
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In: FOOD SCIENCE AND HUMAN WELLNESS. - : KEAI PUBLISHING LTD. - 2213-4530. ; 12:5, s. 1609-1617
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Journal article (peer-reviewed)abstract
- Oysters (Crassostrea gigas) have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their anti-diabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using phosphate-buffered saline and simulated gastrointestinal digestion was performed. The degree of hydrolysis (DH), structural characterization, molecular weight (Mw) distribution, free amino acid, anti-diabetic activity, and antioxidant activity were studied during in vitro simulated gastrointestinal digestion. The results showed that the alpha-glucosidase inhibitory activity, alpha-amylase inhibitory activity, DPPH radical scavenging activity, and ABTS radical scavenging activity of the oyster protein gastrointestinal digest were increased (P < 0.05) from 0 to 33.96%, from 9.17% to 44.22%, from 9.01 mu g trolox/mg protein to 18.48 mu g trolox/mg protein, and from 21.44 mu g trolox/mg protein to 56.21 mu g trolox/mg protein, respectively. Additionally, the DH, beta-turn structure, fluorescence intensity, free amino acid, and short peptide content (Mw < 1 000 Da) increased in the simulated gastrointestinal digestion. These results indicate that the digestive hydrolysates obtained from oyster proteins could be used as natural anti-diabetic and antioxidant agents.
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