| 1. |
- Corbacho, Isaac, et al.
(author)
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Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae
- 2006
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In: Antonie van Leeuwenhoek. - : Springer Science and Business Media LLC. - 0003-6072 .- 1572-9699. ; 89:3-4, s. 485-494
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Journal article (peer-reviewed)abstract
- Abstract The MNN2 gene of S. cerevisiae encodes an α (1,2) mannosyl transferase required for branching the outer chain of N-linked oligosaccharides (Rayner J.C. and Munro S. 1998. J. Biol. Chem. 273: 2683626843) and it also seems to have some effect on the transfer of mannosyl phosphate groups to the inner core (Olivero I. et al. 2000. FEBS Lett. 475: 111116). In order to reveal possible interactions of MNN2 expression with other cellular pathways, we analyzed the transcriptome of the deletion mutant S. cerevisiae mnn2Δ using cDNA microarrays. We found 151 genes that showed an altered expression level of ≥2-fold, 58 of them up-regulated and 93 down-regulated. Quite a high proportion of these genes (29%) encode unclassified proteins. In contrast to other defects affecting the integrity of the cell wall, deletion of MNN2 does not stimulate the expression of any of the genes included in the previously defined cell wall compensatory cluster (Lagorce et al. 2003. J. Biol. Chem. 278: 2034520357). We also found that 15% of the selected genes are related to central metabolic pathways. In addition, the mnn2Δ strain seems to have a certain level of stimulation of DNA processing reactions while some genes involved in intracellular transport pathways are under-regulated
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| 2. |
- Kayingo, G., et al.
(author)
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Accumulation and release of the osmolyte glycerol is independent of the putative MIP channel Spac977.17p in Schizosaccharomyces pombe
- 2004
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In: Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. - 0003-6072 .- 1572-9699. ; 85:2, s. 85-92
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Journal article (peer-reviewed)abstract
- Schizosaccharomyces pombe accumulates glycerol as an osmotic regulatory solute in response to hyper-osmotic conditions. Upon a decrease in the external osmolarity, the intracellular glycerol levels should be adjusted in order to attain osmotic homeostasis. In this study, the patterns and kinetics of glycerol export from S. pombe were investigated. Upon a decrease in external osmolarity, glycerol was rapidly exported from cells to the external medium. The amount of glycerol released from the cells was proportional to the degree of change in the external osmolarity. The export process was well controlled and was not affected by reduced temperature. This points to S. pombe controlling glycerol export using specialized facilitating proteins as has been found in Saccharomyces cerevisiae where a MIP family channel protein Fps1p is involved. Analysis of the S. pombe databases revealed a putative transport protein (Spac977.17p) with homology to glycerol channel proteins of the MIP family. However, expression of the gene into the S. cerevisiae strain lacking a glycerol channel protein (fps1Delta mutant), did not complement the defect in glycerol export during hypo-osmotic stress. Deletion of spac977.17, did not affect glycerol accumulation or release in S. pombe. The patterns and kinetics of glycerol release in the mutant were similar to those of the wild type strains suggesting that the export process is independent of Spac977.17p, the only putative MIP family glycerol channel homologue in S. pombe. While the process of glycerol export in response to hypo-osmotic stress is similar to budding yeast, the underlying molecular mechanism in S. pombe appears distinct from that described in S. cerevisiae. Further studies are needed to elucidate the physiological role of the Spac977.17p channel.
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| 3. |
- Passoth, Volkmar, et al.
(author)
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Cryptococcus cerealis sp nov a psychrophilic yeast species isolated from fermented cereals
- 2009
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In: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology. - Dordrecht, Netherlands : Springer. - 0003-6072 .- 1572-9699. ; 96:4, s. 635-643
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Journal article (peer-reviewed)abstract
- Two yeast strains isolated in 2007 from fermented pig feed were studied, including the analysis of sequences of the D1/D2 and ITS-regions of the rDNA-repeats, their morphology and nutritional physiology. Sequence comparison of the D1/D2 and ITS regions demonstrated that the strains do not belong to any known species. Therefore, a new species, Cryptococcus cerealis with the type strain CBS 10505, is proposed. The species belongs to Filobasidiales (Agaricomycetes, Basidiomycota), and has Cryptococcus saitoi as the closest related species. The new species is psychrophilic, showing significant growth at 4 and 10A degrees C.
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