| 1. |
- Berglund, Björn, et al.
(author)
-
Characterization of extended-spectrum -lactamase-producing Escherichia coli harboring mcr-1 and toxin genes from human fecal samples from China
- 2018
-
In: Future Microbiology. - : FUTURE MEDICINE LTD. - 1746-0913 .- 1746-0921. ; 13:15, s. 1647-1656
-
Journal article (peer-reviewed)abstract
- Aim: To characterize extended-spectrum -lactamase-producing Escherichia coli harboring the colistin resistance gene mcr-1 from human fecal samples collected in 2012 in a rural area of Shandong province, PR China. Materials amp; methods: Whole-genome sequencing and antimicrobial susceptibility testing was performed on 25 mcr-1-positive isolates to determine carriage of antibiotic resistance and virulence genes, diversity and antibiotic resistance profiles. Results: The isolates were highly genetically diverse and carried a large variety of different antibiotic resistance genes. The multidrug-resistance rate was high (96%). Virulence genes associated with intestinal pathogenic E. coli were carried by 32% of the isolates. Conclusion: Further monitoring of the epidemiological situation is necessary to ensure a preparedness for potential emergence of novel, difficult-to-treat strains and awareness of available treatment options.
|
|
| 2. |
- Cohen, A., et al.
(author)
-
A multifaceted 'omics' approach for addressing the challenge of antimicrobial resistance
- 2015
-
In: Future Microbiology. - : Future Medicine Ltd. - 1746-0913 .- 1746-0921. ; 10:3, s. 365-376
-
Journal article (peer-reviewed)abstract
- The inappropriate use of antibiotics has severe global health and economic consequences, including the emergence of antibiotic-resistant bacteria. A major driver of antibiotic misuse is the inability to accurately distinguish between bacterial and viral infections based on currently available diagnostic solutions. A multifaceted 'omics' approach that integrates personalized patient data such as genetic predisposition to infections (genomics), natural microbiota composition and immune response to infection (proteomics and transcriptomics) together with comprehensive pathogen profiling has the potential to help physicians improve their antimicrobial prescribing practices. In this respect, the EU has funded a multidisciplinary project (TAILORED-Treatment) that will develop novel omics-based personalized treatment schemes that have the potential to reduce antibiotic consumption, and help limiting the spread of antibiotic resistance.
|
|
| 3. |
- Dyar, OJ, et al.
(author)
-
Using antibiotics responsibly: are we there yet?
- 2016
-
In: Future microbiology. - : Future Medicine Ltd. - 1746-0921 .- 1746-0913. ; 11:8, s. 1057-1071
-
Journal article (peer-reviewed)abstract
- Problems of antibiotic access and excess coexist in the world today and are compounded by rising rates of antibiotic resistance. We introduce two dimensions of responsibility to this context: responsible individual practices and a broad societal obligation centered on sustainability. Acting on these responsibilities requires recognizing the potential tensions between an individual optimum for antibiotic use and the societal optimum. We relate the tragedy of the commons metaphor to this situation to illustrate the complexity involved, and we draw on real-world experiences in Uganda, India, China and France. We conclude that we must form a global stewardship of antibiotics that can link access, innovation and conservation efforts across countries to ensure sustainable access to effective antibiotics for all who need them.
|
|
| 4. |
- Lood, Rolf, et al.
(author)
-
Localization-triggered bacterial pathogenesis.
- 2015
-
In: Future Microbiology. - : Future Medicine Ltd. - 1746-0921 .- 1746-0913. ; 10:10, s. 1659-1668
-
Research review (peer-reviewed)abstract
- Bacterial infections are becoming an increasing problem worldwide and there is a need for a deeper understanding of how bacteria turn pathogenic. Here, we suggest that one answer may be found by taking into account the localization of the bacteria, both at an anatomical level and at a microenvironment level. Both commensals and traditional pathogens alter their interaction with the human host depending on the local surroundings - turning either more or less virulent. These localization effects could derive from the characteristics of different anatomical sites but also from local differences within a microenvironment. In order to understand the adaptive functions of bacterial virulence factors, we need to study the bacteria in the environments where they have evolved.
|
|
| 5. |
- Selegård, Robert, et al.
(author)
-
Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis
- 2019
-
In: Future Microbiology. - : Future Medicine. - 1746-0913 .- 1746-0921. ; 14:3, s. 195-206
-
Journal article (peer-reviewed)abstract
- AIM: Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis.MATERIALS & METHODS: The effects on membrane integrity were studied using liposomes and viable bacteria, respectively.RESULTS: We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes. The PlnEF and PlnJK displayed similar mechanisms by targeting and disrupting the bacterial cell membrane. Interestingly, Pln enhanced the effects of different antibiotics by 30- to 500-fold.CONCLUSION: This study shows that Pln in combination with low concentrations of antibiotics is efficient against S. epidermidis and may be developed as potential treatment of infections.
|
|
| 6. |
- Shadnezhad, Azadeh, et al.
(author)
-
EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae
- 2016
-
In: Future Microbiology. - : Future Medicine Ltd. - 1746-0913 .- 1746-0921. ; 11:6, s. 721-736
-
Journal article (peer-reviewed)abstract
- Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD). Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd. Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium. Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.
|
|
