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- Bhaskaran, Nimesh, et al.
(author)
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Novel post-translational modifications of Smad2 identified by mass spectrometry
- 2008
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In: Central European Journal of Biology. - : Walter de Gruyter GmbH. - 1895-104X .- 1644-3632. ; 3:4, s. 359-370
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Journal article (peer-reviewed)abstract
- Smad2 is a crucial component of transforming growth factor-b (TGFb) signaling, and is involved in the regulation of cell proliferation,death and differentiation. Phosphorylation, ubiquitylation and acetylation of Smad2 have been found to regulate its activity. We usedmass spectrometry to search for novel post-translational modifications (PTMs) of Smad2. Peptide mass fingerprinting (PMF) indicatedthat Smad2 can be acetylated, methylated, citrullinated, phosphorylated and palmitoylated. Sequencing of selected peptides validatedmethylation at Gly122 and hydroxylation at Trp18 of Smad2. We also observed a novel, so far unidentified modification at Tyr128 andTyr151. Our observations open for further exploration of biological importance of the detected PTMs.
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