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- Bergman, Anders, et al.
(author)
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Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach Leaves.
- 1980
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In: Plant Physiology. - Rockville, USA : AMER SOC PLANT PHYSIOLOGISTS. - 0032-0889 .- 1532-2548. ; 66:3, s. 442-445
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Journal article (peer-reviewed)abstract
- Mitochondria from green leaves of spinach have been prepared using a three-step procedure involving differential centrifugation, partition in an aqueous dextran polyethylene glycol two-phase system and Percoll gradient centrifugation. The mitochondrial fractions after the different steps of purification were compared. The final mitochondrial preparation was totally free from chloroplast material measured as chlorophyll content. The enrichment of mitochondria in relation to peroxisomes and microsomes was approximately 12 and 33 times, respectively, based on NAD:isocitrate dehydrogenase activity, glycolate oxidase activity, and NADPH:cytochrome c oxidoreductase activity. The apparent intactness of the inner and the outer mitochondrial membranes was higher than 90% as measured by latency of enzyme activities. The mitochondria showed high respiratory rates with respiratory control and the ADP/O ratios approached the theoretical limits.
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| 2. |
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| 3. |
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| 4. |
- Gardeström, Per, 1950-, et al.
(author)
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Oxidation of Glycine via the Respiratory Chain in Mitochondria Prepared from Different Parts of Spinach.
- 1980
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In: Plant Physiology. - 0032-0889 .- 1532-2548. ; 65:2, s. 389-91
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Journal article (peer-reviewed)abstract
- Mitochondria were prepared from roots, stalks, leaves, and leaf veins of spinach. The mitochondrial preparations were examined for their ability to oxidize glycine via the respiratory chain. It is shown that the glycine-oxidizing capacity is restricted to photosynthetically active tissue. The activity is present in mitochondria from the green parts of the leaves, but not in mitochondria from roots, stalks, or leaf veins.
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