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- Fang, Qinghua, et al.
(author)
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Comparative Analysis on Abnormal Methylome of Differentially Expressed Genes and Disease Pathways in the Immune Cells of RA and SLE
- 2021
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 12
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Journal article (peer-reviewed)abstract
- We identified abnormally methylated, differentially expressed genes (DEGs) and pathogenic mechanisms in different immune cells of RA and SLE by comprehensive bioinformatics analysis. Six microarray data sets of each immune cell (CD19+ B cells, CD4+ T cells and CD14+ monocytes) were integrated to screen DEGs and differentially methylated genes by using R package "limma." Gene ontology annotations and KEGG analysis of aberrant methylome of DEGs were done using DAVID online database. Protein-protein interaction (PPI) network was generated to detect the hub genes and their methylation levels were compared using DiseaseMeth 2.0 database. Aberrantly methylated DEGs in CD19+ B cells (173 and 180), CD4+ T cells (184 and 417) and CD14+ monocytes (193 and 392) of RA and SLE patients were identified. We detected 30 hub genes in different immune cells of RA and SLE and confirmed their expression using FACS sorted immune cells by qPCR. Among them, 12 genes (BPTF, PHC2, JUN, KRAS, PTEN, FGFR2, ALB, SERB-1, SKP2, TUBA1A, IMP3, and SMAD4) of RA and 12 genes (OAS1, RSAD2, OASL, IFIT3, OAS2, IFIH1, CENPE, TOP2A, PBK, KIF11, IFIT1, and ISG15) of SLE are proposed as potential biomarker genes based on receiver operating curve analysis. Our study suggests that MAPK signaling pathway could potentially differentiate the mechanisms affecting T- and B- cells in RA, whereas PI3K pathway may be used for exploring common disease pathways between RA and SLE. Compared to individual data analyses, more dependable and precise filtering of results can be achieved by integrating several relevant data sets.
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- Liu, Zhouying, et al.
(author)
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Exploring askers' switching from free to paid social Q&A services : A perspective on the push-pull-mooring framework
- 2021
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In: Information Processing & Management. - : Elsevier BV. - 0306-4573 .- 1873-5371. ; 58:1
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Journal article (peer-reviewed)abstract
- The purpose of this study is to explore the factors that prompt askers to switch from free to paid social question-and-answer (SQA) services. Prior studies have investigated users' motivations and participation in free and paid SQA services; however, little attention has been paid to askers' switching behavior. We empirically analyzed the content of qualitative interviews from 64 askers on a well-known SQA platform in China. Based on the push-pull-mooring framework, we identified and classified factors that influenced askers' to switch from free to paid Q&A services, using the critical incident technique, after which we calculated the entropy weights of the 16 subcategories before and after the switch, using the entropy weight method. The findings suggest that askers' switching behavior was influenced by push factors (i.e., dissatisfaction with the free SQA service), pull factors (i.e., satisfaction with the paid SQA service), and mooring factors (i.e., social factors, personal factors, situational factors). Moreover, the findings show that the effects of these factors vary significantly before and after a switch. Dissatisfaction with the quality of information from the free SQA service would influence users before a switch, whereas satisfaction with the quality of information from the paid SQA service would influence them after a switch. In terms of mooring factors, the effects of social and personal factors on askers' switching behavior, especially subjective norms and cognitive lock-in, turn out to be less significant after a switch, whereas the effect of trust is more significant. Besides, the effects of situational factors are more or less the same before and after a switch. To the best of our knowledge, this paper is one of the first attempts to explore factors that affect askers' switching behavior and to shed light on the managerial strategies of paid SQA services.
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- Xie, Wenjing, et al.
(author)
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Unexpected Dioxin Formation During Digestion of Soil with Oxidizing Acids
- 2023
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In: Environmental Science and Technology. - : AMER CHEMICAL SOC. - 0013-936X .- 1520-5851. ; 57:39, s. 14717-14725
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Journal article (peer-reviewed)abstract
- Dioxins, such as polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs), are among the most toxic unintentionally produced persistent organic pollutants, and their emission is of great concern. Herein, we discovered abundant dioxin formation in soil and various organic carbon-containing matrices after digestion with aqua regia. Sigma 17PCDD/Fs concentrations were in the range of 66.6-142,834 pg/g dw (5.6-17,021 pg WHO2005-TEQ/g dw) in 19 soil samples after digestion with aqua regia for 6 h. Sigma 17PCDD/Fs concentration was significantly and positively correlated with soil organic carbon content (R-2 = 0.89; p < 0.01). Compared with cellulose and lignin, humic acid served as an important organic matter component that was converted to PCDD/Fs during soil digestion. Strong oxidation and production of reactive chlorine by aqua regia may be the key factors in the formation of PCDD/Fs. The yearly emission of PCDD/Fs due to digestion with strong acids by the inspection and testing industry was estimated to be 83.8 g TEQ in China in 2021 based on the highest level, which was similar to 0.9% of the total dioxin inventory in China. Great attention should be paid to unexpected dioxin formation during digestion processes considering the potential risk of release from laboratories and enterprises.
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- Zhang, Wei, et al.
(author)
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Surgical treatment of low-grade brain tumors associated with epilepsy
- 2020
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In: NOVEL THERAPEUTIC ADVANCES IN GLIOBLASTOMA. - LONDON ENGLAND : Elsevier. - 9780128211144 ; , s. 171-183
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Book chapter (peer-reviewed)abstract
- Objective: To explore the strategy of surgical treatment of low-grade brain tumors associated with epilepsy. Methods: Clinical data of 158 patients with low-grade brain tumors were collected from January 2011 to December 2017 in Guangdong Sanjiu brain hospital. All patients received Preoperative evaluation. Lesion site: 18 cases were located in multiple cerebral lobes, 10 cases were in the functional zones, 130 cases were in the non-functional zones (including 74 cases were in the medial of temporal lobe). The surgical strategy included subtotal resection, gross-total resection and enlarged resection. Postoperative effects were evaluated by Engel classification. Results: A total of 158 patients underwent surgical treatment, among these patients, only 1 patient underwent intracranial electrode implantation. Surgical methods: 34 cases of subtotal resection, 3 cases of gross-total resection, 119 cases of enlarged resection (including Anterior temporal lobectomy in 74 cases) and 2 case of Selective hippocampal amygdalectomy. The final pathology suggested that there are 74 cases of ganglionglioma, 25 cases of dysembryoplastic neuroepithelial tumors, 9 cases of pilocytic astrocytoma, 16 cases of oligodendroglioma, 10 cases of pleomorphic xanthoastrocytoma, 4 case of diffuse astrocytoma, 9 cases of unclassified astrocytoma, 11 case of oligoastrocytoma. The follow-up time was between 1 and 7 years, with an average of 3.44 +/- 1.77 years. Postoperative recovery: 147 patients had an Engel Class I outcome, 10 patients were in Engel Class II, 1 patient was in Class IV. Conclusion: The strategy of surgical treatment of low-grade brain tumors associated with epilepsy should pay more attention to the preoperative assessment of the epileptogenic zone. The tumor is not exactly the same as the epileptogenic zone, and the strategy of surgical treatment depends on the tumor feature as well as whether it was located in temporal lobe or involved in functional areas.
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