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Träfflista för sökning "WFRF:(Deckers M.) srt2:(2010-2014)"

Search: WFRF:(Deckers M.) > (2010-2014)

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1.
  • Bustin, Stephen A., et al. (author)
  • The need for transparency and good practices in the qPCR literature
  • 2013
  • In: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 10:11, s. 1063-1067
  • Journal article (other academic/artistic)abstract
    • Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
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2.
  • Vanden Berghe, T, et al. (author)
  • Necroptosis, necrosis and secondary necrosis converge on similar cellular disintegration features
  • 2010
  • In: CELL DEATH AND DIFFERENTIATION. - : Nature Publishing Group. - 1350-9047 .- 1476-5403. ; 17:6, s. 922-930
  • Journal article (peer-reviewed)abstract
    • Necroptosis, necrosis and secondary necrosis following apoptosis represent different modes of cell death that eventually result in similar cellular morphology including rounding of the cell, cytoplasmic swelling, rupture of the plasma membrane and spilling of the intracellular content. Subcellular events during tumor necrosis factor (TNF)-induced necroptosis, H2O2-induced necrosis and anti-Fas-induced secondary necrosis were studied using high-resolution time-lapse microscopy. The cellular disintegration phase of the three types of necrosis is characterized by an identical sequence of subcellular events, including oxidative burst, mitochondrial membrane hyperpolarization, lysosomal membrane permeabilization and plasma membrane permeabilization, although with different kinetics. H2O2-induced necrosis starts immediately by lysosomal permeabilization. In contrast, during TNF-mediated necroptosis and anti-Fas-induced secondary necrosis, this is a late event preceded by a defined signaling phase. TNF-induced necroptosis depends on receptor-interacting protein-1 kinase, mitochondrial complex I and cytosolic phospholipase A(2) activities, whereas H2O2-induced necrosis requires iron-dependent Fenton reactions.
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