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- Jungebluth, P, et al.
(författare)
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Retraction Statement
- 2023
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Ingår i: Respiration. - : S. Karger AG. - 1423-0356 .- 0025-7931. ; 102:5, s. 402-404
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Lewicka, M, et al.
(författare)
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Recombinant Spider Silk Protein Matrices Facilitate Differentiation of Neural Stem Cells Into Mature and Functional Neurons
- 2021
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Ingår i: FRONTIERS IN MATERIALS. - : Frontiers Media SA. - 2296-8016. ; 7
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Neural stem cells (NSCs) show great promise in drug discovery and clinical application. Yet few efforts have been made to optimize biocompatible materials for such cells to be expanded and used in clinical conditions. We have previously demonstrated that NSCs are readily cultured on substrates of certain recombinant spider silk protein without addition of animal- or human-derived components. The question remains however whether this material allows differentiation into functional neurons, and whether such differentiation can take place also when the NSCs are cultured not only upon but also within the biodegradable material. Here we demonstrate that “foam”-like structures generated from recombinant spider silk protein (4RepCT) provided excellent matrices for the generation and multicellular analysis of functional excitatory neurons from NSCs without addition of animal- or human-derived components. NSCs isolated from the cerebral cortices of rat embryos were cultured at either 4RepCT matrices shaped as foam-like structures without coating, or on conventional polystyrene plates coated with poly-L-ornithine and fibronectin. Upon treatment with recombinant proteins including the extracellular signaling factor BMP4 or a combination of BMP4 and the signaling factor Wnt3a, the cortical NSCs cultured in 4RepCT foam-like structures differentiated efficiently into neurons that responded to glutamate receptor agonists, such as AMPA, to the same extent as control cultures. Matrices derived from recombinant spider silk proteins thus provide a functional microenvironment for neural stem cells with little or no animal- or human-derived components and can be employed in the development of new strategies in stem cell research and tissue engineering.
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- Risto, Anton, et al.
(författare)
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Colectomy reconstruction for ulcerative colitis in Sweden and England: a multicenter prospective comparison between ileorectal anastomosis and ileal pouch-anal anastomosis after colectomy in patients with ulcerative colitis. (CRUISE-study)
- 2023
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Ingår i: BMC Surgery. - : BMC. - 1471-2482. ; 23:1
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundThere are no prospective trials comparing the two main reconstructive options after colectomy for Ulcerative colitis, ileal pouch anal anastomosis and ileorectal anastomosis. An attempt on a randomized controlled trial has been made but after receiving standardized information patients insisted on choosing operation themselves.MethodsAdult Ulcerative colitis patients subjected to colectomy eligible for both ileal pouch anastomosis and ileorectal anastomosis are asked to participate and after receiving standardized information the get to choose reconstructive method. Patients declining reconstruction or not considered eligible for both methods will be followed as controls. The CRUISE study is a prospective, non-randomized, multi-center, open-label, controlled trial on satisfaction, QoL, function, and complications between ileal pouch anal anastomosis and ileorectal anastomosis.DiscussionReconstruction after colectomy is a morbidity-associated as well as a resource-intensive activity with the sole purpose of enhancing function, QoL and patient satisfaction. The aim of this study is to provide the best possible information on the risks and benefits of each reconstructive treatment.
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- Ruppel, M. M., et al.
(författare)
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Organic Compounds, Radiocarbon, Trace Elements and Atmospheric Transport Illuminating Sources of Elemental Carbon in a 300-Year Svalbard Ice Core
- 2023
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Ingår i: Journal of Geophysical Research - Atmospheres. - 2169-897X .- 2169-8996. ; 128:16
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Tidskriftsartikel (refereegranskat)abstract
- Black carbon (BC) particles produced by incomplete combustion of biomass and fossil fuels warm the atmosphere and decrease the reflectivity of snow and ice, hastening their melt. Although the significance of BC in Arctic climate change is widely acknowledged, observations on its deposition and sources are few. We present BC source types in a 300-year (1700-2005) Svalbard ice core by analysis of particle-bound organic compounds, radiocarbon, and trace elements. According to the radiocarbon results, 58% of the deposited elemental carbon (EC, thermal-optical proxy of BC) is of non-fossil origin throughout the record, while the organic compounds suggest a higher percentage (68%). The contribution of fossil fuels to EC is suggested to have been elevated between 1860 and 1920, particularly based on the organics and trace element data. A second increase in fossil fuel sources seems to have occurred near the end of the record: according to radiocarbon measurements between 1960 and 1990, while the organics and trace element data suggest that the contribution of fossil fuels has increased since the 1970s to the end of the record, along with observed increasing EC deposition. Modeled atmospheric transport between 1948 and 2004 shows that increasing EC deposition observed at the glacier during that period can be associated with increased atmospheric transport from Far East Asia. Further observational BC source data are essential to help target climate change mitigation efforts. The combination of robust radiocarbon with organic compound analyses requiring low sample amounts seems a promising approach for comprehensive Arctic BC source apportionment.
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