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- Habenicht, Anja, et al.
(author)
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Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments
- 2002
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In: Chemical Physics Letters. ; 354:5-6, s. 367-75
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Journal article (peer-reviewed)abstract
- An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.
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