| 1. |
- Assenhöj, Maria, et al.
(author)
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Protein interaction, monocyte toxicity and immunogenic properties of cerium oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles–an interdisciplinary approach
- 2021
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In: Nanotoxicology. - : Taylor and Francis Ltd.. - 1743-5390 .- 1743-5404. ; 15:8, s. 1035-1038
-
Journal article (peer-reviewed)abstract
- Metal oxide nanoparticles are widely used in both consumer products and medical applications, but the knowledge regarding exposure-related health effects is limited. However, it is challenging to investigate nanoparticle interaction processes with biological systems. The overall aim of this project was to improve the possibility to predict exposure-related health effects of metal oxide nanoparticles through interdisciplinary collaboration by combining workflows from the pharmaceutical industry, nanomaterial sciences, and occupational medicine. Specific aims were to investigate nanoparticle-protein interactions and possible adverse immune reactions. Four different metal oxide nanoparticles; CeOx nanocrystals with 5% or 14% Gd, Co3O4, and Fe2O3, were characterized by dynamic light scattering and high-resolution transmission electron microscopy. Nanoparticle-binding proteins were identified and screened for HLA-binding peptides in silico. Monocyte interaction with nanoparticle–protein complexes was assessed in vitro. Herein, for the first time, immunogenic properties of nanoparticle-binding proteins have been characterized. The present study indicates that especially Co3O4-protein complexes can induce both ‘danger signals’, verified by the production of inflammatory cytokines and simultaneously bind autologous proteins, which can be presented as immunogenic epitopes by MHC class II. The clinical relevance of these findings should be further evaluated to investigate the role of metal oxide nanoparticles in the development of autoimmune disease. The general workflow identified experimental difficulties, such as nanoparticle aggregate formation and a lack of protein-free buffers suitable for particle characterization, protein analyses, as well as for cell studies. This confirms the importance of future interdisciplinary collaborations. © 2021 The Author(s).
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| 2. |
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| 3. |
- Eneroth, Hanna, et al.
(author)
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Environmental impact of coffee, tea and cocoa – data collection for a consumer guide for plant-based foods
- 2022
-
Reports (other academic/artistic)abstract
- In 2020, WWF launched a consumer guide on plant-based products targeting Swedish consumers. The development of the guide is described in a journal paper (Karlsson Potter & Röös, 2021) and the environmental impact of different plant based foods was published in a report (Karlsson Potter, Lundmark, & Röös, 2020). This report was prepared for WWF Sweden to provide scientific background information for complementing the consumer guide with information on coffee, tea and cocoa. This report includes quantitative estimations for several environmental categories (climate, land use, biodiversity and water use) of coffee (per L), tea (per L) and cocoa powder (per kg), building on the previously established methodology for the consumer guide. In addition, scenarios of consumption of coffee, tea and cocoa drink with milk/plant-based drinks and waste at household level, are presented. Tea, coffee and cacao beans have a lot in common. They are tropical perennial crops traditionally grown in the shade among other species, i.e. in agroforestry systems. Today, the production in intensive monocultures has negative impact on biodiversity. Re-introducing agroforestry practices may be part of the solution to improve biodiversity in these landscapes. Climate change will likely, due to changes in temperature, extreme weather events and increases in pests and disease, alter the areas where these crops can be grown in the future. A relatively high ratio of the global land used for coffee, tea and cocoa is certified according to sustainability standards, compared to other crops. Although research on the implications of voluntary standards on different outcomes is inconclusive, the literature supports that certifications have a role in incentivizing more sustainable farming. Coffee, tea and cocoa all contain caffeine and have a high content of bioactive compounds such as antioxidants, and they have all been associated with positive health outcomes. While there is a strong coffee culture in Sweden and coffee contributes substantially to the environmental impact of our diet, tea is a less consumed beverage. Cocoa powder is consumed as a beverage, but substantial amounts of our cocoa consumption is in the form of chocolate. Roasted ground coffee on the Swedish market had a climate impact of 4.0 kg CO2e per kg powder, while the climate impact of instant coffee powder was 11.5 kg CO2e per kg. Per litre, including the energy use for making the coffee, the total climate impact was estimated to 0.25 kg CO2e per L brewed coffee and 0.16 kg CO2e per L for instant coffee. Less green coffee beans are needed to produce the same amount of ready to drink coffee from instant coffee than from brewed coffee. Tea had a climate impact of approximately 6.3 kg CO2 e per kg dry leaves corresponding to an impact of 0.064 CO2e per L ready to drink tea. In the assessment of climate impact per cup, tea had the lowest impact with 0.013 kg CO2e, followed by black instant coffee (0.024 kg CO2e), black coffee (0.038 kg CO2e), and cocoa drink made with milk (0.33 kg CO2e). The climate impact of 1kg cocoa powder on the Swedish market was estimated to 2.8 kg CO2e. Adding milk to coffee or tea increases the climate impact substantially. The literature describes a high proportion of the total climate impact of coffee from the consumer stage due to the electricity used by the coffee machine. However, with the Nordic low-carbon energy mix, the brewing and heating of water and milk contributes to only a minor part of the climate impact of coffee. As in previous research, coffee also had a higher land use, water use and biodiversity impact than tea per L beverage. Another factor of interest at the consumer stage is the waste of prepared coffee. Waste of prepared coffee contributes to climate impact through the additional production costs and electricity for preparation, even though the latter was small in our calculations. The waste of coffee and tea at Summary household level is extensive and measures to reduce the amount of wasted coffee and tea could reduce the environmental impact of Swedish hot drink consumption. For the final evaluation of coffee and tea for the consumer guide, the boundary for the fruit and vegetable group was used. The functional unit for coffee and tea was 1 L prepared beverage without any added milk or sweetener. In the guide, the final evaluation of conventionally grown coffee is that it is ‘yellow’ (‘Consume sometimes’), and for organic produce, ‘light green’ (‘Please consume). The evaluation of conventionally grown tea is that it is ‘light green’, and for organic produce, ‘dark green’ (‘Preferably consume this’). For cocoa, the functional unit is 1 kg of cocoa powder and the boundary was taken from the protein group. The final evaluation of conventionally grown cocoa is that it is ‘orange’ (‘Be careful’), and for organically produced cocoa, ‘light green’.
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| 4. |
- Karlsson, Hanna K., et al.
(author)
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Chemical absorption of carbon dioxide in non-aqueous systems using the amine 2-amino-2-methyl-1-propanol in dimethyl sulfoxide and N-methyl-2-pyrrolidone
- 2021
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In: Separation and Purification Technology. - : Elsevier BV. - 1383-5866. ; 256
-
Journal article (peer-reviewed)abstract
- Non-aqueous amine systems have been suggested as energy-efficient alternatives to conventional aqueous amine systems in post-combustion carbon capture, as low regeneration temperatures can be achieved. The solubility of CO2 and heat of absorption in non-aqueous systems were studied using the sterically hindered amine 2-amino-2-methyl-1-propnaol (AMP) in the organic solvent dimethyl sulfoxide (DMSO). 13C NMR was used to study the product species in solution as CO2 reacts with AMP in either DMSO or N-methyl-2-pyrrolidone (NMP). The solubility of CO2 in AMP/DMSO showed that low loadings could be achieved at 80–88 °C, indicating that regeneration can be carried out at lower temperatures than in conventional aqueous systems. Precipitation occurred at 25 wt% AMP in DMSO, increasing the overall capacity of the system. The heat of absorption decreased with increasing temperature, and was explained by physical absorption dominating the absorption mechanism at higher temperatures. This was also confirmed by the results of NMR, as less chemically absorbed species were observed at higher temperatures. The reaction products observed in AMP/DMSO and AMP/NMP were identified as the AMP carbamate, bicarbonate from water impurities, and the AMP carbonate from CO2 reacting with the hydroxyl group of AMP.
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| 5. |
- Karlsson, Hanna K., et al.
(author)
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Evaluation of the effect of water on CO2 absorption in AMP and DMSO systems
- 2024
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In: Separation and Purification Technology. - 1873-3794. ; 341
-
Journal article (peer-reviewed)abstract
- Non-aqueous precipitating amine systems for carbon capture allows for CO2 desorption at lower temperatures than conventional aqueous amine systems and can potentially reduce the energy requirement for regeneration. In this work, the influence of water accumulation that may arise from humid gases entering the absorption column was investigated for absorption systems containing 2-amino-2-methyl-1-propanol (AMP) and dimethyl sulfoxide (DMSO). The physical solubility of CO2 decreased with increasing water concentration, as expected from the lower solubility of CO2 in water than in DMSO. The CO2 loading capacity was increased with the addition of water, resulting from formation of bicarbonate with water present in the system. Low lean loadings of 0.1 mol CO2/mol AMP and precipitation was observed in 23 wt% AMP/DMSO with 9 wt% added water, suggesting that some water accumulation might be tolerable while still maintaining the desired properties of the absorption system. NMR was used to study the CO2 reaction products at 30–88 °C. The results suggested that 88 °C can be used for regeneration of the system even with water accumulated in the system. At 80 °C formation of the tentatively assigned species 4,4-dimethyl-1,3-oxazolidin-2-one was observed, indicating that thermal degradation of AMP may occur above this temperature.
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| 6. |
- Karlsson, Hanna L., et al.
(author)
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Health hazards of particles in additive manufacturing : a cross-disciplinary study on reactivity, toxicity and occupational exposure to two nickel-based alloys
- 2023
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In: Scientific Reports. - : Springer Nature. - 2045-2322. ; 13:1
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Journal article (peer-reviewed)abstract
- The increasing use of additive manufacturing (AM) techniques (e.g., 3D-printing) offers many advantages but at the same time presents some challenges. One concern is the possible exposure and health risk related to metal containing particles of different sizes. Using the nickel-based alloys Hastelloy X (HX) and Inconel 939 (IN939) as a case, the aim of this cross-disciplinary study was to increase the understanding on possible health hazards and exposure. This was done by performing in-depth characterization of virgin, reused and condensate powders, testing in vitro toxicity (cytotoxicity, genotoxicity, oxidative stress), and measuring occupational airborne exposure. The results showed limited metal release from both HX and IN939, and slightly different surface composition of reused compared to virgin powders. No or small effects on the cultured lung cells were observed when tested up to 100 µg/mL. Particle background levels in the printing facilities were generally low, but high transient peaks were observed in relation to sieving. Furthermore, during post processing with grinding, high levels of nanoparticles (> 100,000 particles/cm3) were noted. Urine metal levels in AM operators did not exceed biomonitoring action limits. Future studies should focus on understanding the toxicity of the nanoparticles formed during printing and post-processing.
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| 7. |
- Lindell, Ellen, 1979, et al.
(author)
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Benign paroxysmal positional vertigo, dizziness, and health-related quality of life among older adults in a population-based setting
- 2021
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In: European Archives of Oto-Rhino-Laryngology. - : Springer Science and Business Media LLC. - 0937-4477 .- 1434-4726. ; 278:5, s. 1637-1644
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Journal article (peer-reviewed)abstract
- Purpose Dizziness may affect quality of life in a negative way and contribute to falls. The aim of this study was to investigate and compare 75 years old with dizziness caused by benign paroxysmal positional vertigo (BPPV) to those with general dizziness/impaired balance (non-BPPV related) and to those reporting no dizziness, regarding health-related quality of life (HRQL), falls, tiredness, and walking speed in a population-based setting. Method A cross-sectional population-based sample, including 671 75 years old (398 women, 273 men), was investigated for BPPV, dizziness symptoms, falls, and walking speed. HRQL was assessed using the 36-item Short Form-36 Health Survey (SF-36). Result A total of 67 persons (10%) had symptoms of BPPV with 11 (1.6%) having nystagmus when tested for BPPV. Having BPPV as well as general dizziness/impaired balance was associated with reduced HRQL, more tiredness, enhanced number of falls, and lower walking speed. Furthermore, the risk of having BPPV increased fourfold if symptoms of dizziness when turning in bed was reported. Conclusion Having problems with dizziness is common among senior citizens where BPPV can be an unrecognized cause of dizziness that may impact HRQL and overall well-being. As BPPV is common among older adults, and is potentially curable through reposition maneuvers, it is important to liberally test for, and treat the condition. Information about dizziness when turning in bed can help to pinpoint persons with enhanced risk for BPPV also on a population-based level.
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| 8. |
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| 9. |
- Ran, Ylva, et al.
(author)
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Environmental assessment of diets: overview and guidance on indicator choice
- 2024
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In: The Lancet Planetary Health. - : Elsevier BV. - 2542-5196. ; 8:3, s. e172-e187
-
Research review (peer-reviewed)abstract
- Comprehensive but interpretable assessment of the environmental performance of diets involves choosing a set of appropriate indicators. Current knowledge and data gaps on the origin of dietary foodstuffs restrict use of indicators relying on site-specific information. This Personal View summarises commonly used indicators for assessing the environmental performance of diets, briefly outlines their benefits and drawbacks, and provides recommendations on indicator choices for actors across multiple fields involved in activities that include the environmental assessment of diets. We then provide recommendations on indicator choices for actors across multiple fields involved in activities that use environmental assessments, such as health and nutrition experts, policy makers, decision makers, and private-sector and public-sector sustainability officers. We recommend that environmental assessment of diets should include indicators for at least the five following areas: climate change, biosphere integrity, blue water consumption, novel entities, and impacts on natural resources (especially wild fish stocks), to capture important environmental trade-offs. If more indicators can be handled in the assessment, indicators to capture impacts related to land use quantity and quality and green water consumption should be used. For ambitious assessments, indicators related to biogeochemical flows, stratospheric ozone depletion, and energy use can be added.
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| 10. |
- Ran, Ylva, et al.
(author)
-
Environmental assessment of diets: overview and guidance on indicator choice
- 2024
-
In: The Lancet Planetary Health. - : ELSEVIER SCI LTD. - 2542-5196. ; 8:3, s. e172-e187
-
Research review (peer-reviewed)abstract
- Comprehensive but interpretable assessment of the environmental performance of diets involves choosing a set of appropriate indicators. Current knowledge and data gaps on the origin of dietary foodstuffs restrict use of indicators relying on site-specific information. This Personal View summarises commonly used indicators for assessing the environmental performance of diets, briefly outlines their benefits and drawbacks, and provides recommendations on indicator choices for actors across multiple fields involved in activities that include the environmental assessment of diets. We then provide recommendations on indicator choices for actors across multiple fields involved in activities that use environmental assessments, such as health and nutrition experts, policy makers, decision makers, and private-sector and public-sector sustainability officers. We recommend that environmental assessment of diets should include indicators for at least the five following areas: climate change, biosphere integrity, blue water consumption, novel entities, and impacts on natural resources (especially wild fish stocks), to capture important environmental trade-offs. If more indicators can be handled in the assessment, indicators to capture impacts related to land use quantity and quality and green water consumption should be used. For ambitious assessments, indicators related to biogeochemical flows, stratospheric ozone depletion, and energy use can be added.
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| 11. |
- Ran, Ylva, et al.
(author)
-
Environmental assessment of diets: overview and guidance on indicator choice
- 2024
-
In: The Lancet Planetary Health. - 2542-5196. ; 8:3, s. e172-e187
-
Journal article (peer-reviewed)abstract
- Comprehensive but interpretable assessment of the environmental performance of diets involves choosing a set of appropriate indicators. Current knowledge and data gaps on the origin of dietary foodstuffs restrict use of indicators relying on site-specific information.This Personal View summarises commonly used indicators for assessing the environmental performance of diets, briefly outlines their benefits and drawbacks, and provides recommendations on indicator choices for actors across multiple fields involved in activities that include the environmental assessment of diets.We then provide recommendations on indicator choices for actors across multiple fields involved in activities that use environmental assessments, such as health and nutrition experts, policy makers, decision makers, and private-sector and public-sector sustainability officers. We recommend that environmental assessment of diets should include indicators for at least the five following areas: climate change, biosphere integrity, blue water consumption, novel entities, and impacts on natural resources (especially wild fish stocks), to capture important environmental trade-offs.If more indicators can be handled in the assessment, indicators to capture impacts related to land use quantity and quality and green water consumption should be used. For ambitious assessments, indicators related to biogeochemical flows, stratospheric ozone depletion, and energy use can be added.
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| 12. |
- Ran, Ylva, et al.
(author)
-
Environmental assessment of diets: overview and guidance on indicator choice
- 2024
-
In: The Lancet Planetary Health. - : ELSEVIER SCI LTD. - 2542-5196. ; 8:3, s. e172-e187
-
Journal article (peer-reviewed)abstract
- Comprehensive but interpretable assessment of the environmental performance of diets involves choosing a set of appropriate indicators. Current knowledge and data gaps on the origin of dietary foodstuffs restrict use of indicators relying on site-specific information. This Personal View summarises commonly used indicators for assessing the environmental performance of diets, briefly outlines their benefits and drawbacks, and provides recommendations on indicator choices for actors across multiple fields involved in activities that include the environmental assessment of diets. We then provide recommendations on indicator choices for actors across multiple fields involved in activities that use environmental assessments, such as health and nutrition experts, policy makers, decision makers, and privatesector and public-sector sustainability officers. We recommend that environmental assessment of diets should include indicators for at least the five following areas: climate change, biosphere integrity, blue water consump tion, novel entities, and impacts on natural resources (especially wild fish stocks), to capture important environ mental trade-offs. If more indicators can be handled in the assessment, indicators to capture impacts related to land use quantity and quality and green water consumption should be used. For ambitious assessments, indicators related to biogeochemical flows, stratospheric ozone depletion, and energy use can be added.
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| 13. |
- Woksepp, Hanna, et al.
(author)
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Dissemination of carbapenemase-producing Enterobacterales through wastewater and gulls at a wastewater treatment plant in Sweden
- 2023
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In: Science of the Total Environment. - : Elsevier. - 0048-9697 .- 1879-1026. ; 886
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Journal article (peer-reviewed)abstract
- Here we report the detection of carbapenemase-producing Enterobacterales (CPE) isolated from Swedish wastewater and gull faeces. CPE have not been detected in samples from animals in Sweden preceding this report. Sampling of wastewater treatment plant (WWTP) inlet and outlet, sedimentation basins, surface seawater from key aquatic bird habitats and freshly deposited gull faeces was done on six separate occasions during May to September 2021. Following broth enrichment, selective screening of putative CPE was performed on mSuperCarbaTM (CHROMagar). Species identification was done with MALDI-TOF. Antimicrobial susceptibility testing was performed according to EUCAST. In total, seventeen CPE were verified by genome sequencing carrying blaGES-5, blaIMI-3, blaOXA-181 or blaOXA-244. The blaGES-5 was carried on IncP plasmids in four different species; Escherichia coli ST10 isolated from WWTP outlet, Raoultella ornithinolytica isolated from WWTP inlet, outlet and sedimentation basins as well as gull faeces collected at the WWTP and Klebsiella spp. isolates from WWTP inlet and outlet. The genetic environment surrounding blaGES-5 was similar in two Citrobacter freundii causing human infections. The blaIMI-3 was carried on IncFII(Yp) plasmids in four Enterobacter ludwigii, isolated from WWTP outlet and gull faeces collected at a recreational city park 2 km from the WWTP. The blaOXA-181 was located on a COLKP3 plasmid found in an E. coli, while blaOXA-244 was chromosomally located in an E. coli ST10, both isolated from WWTP inlet. Phylogenetic analysis of R. ornithinolytica and E. ludwigii isolates indicate that the gulls carried strains related to those identified in the WWTP samples. The results thus add to the increasing evidence of WWTPs as anthropogenic reservoirs for mobile genetic elements with antibiotic-resistance functionality. Such environments could profoundly impact the dissemination and spread of such genetic elements via for example aquatic birds, thereby warranting further study and surveillance.
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| 14. |
- Abdellah, Tebani, et al.
(author)
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Integration of molecular profiles in a longitudinal wellness profiling cohort.
- 2020
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In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11:1
-
Journal article (peer-reviewed)abstract
- An important aspect of precision medicine is to probe the stability in molecular profiles among healthy individuals over time. Here, we sample a longitudinal wellness cohort with 100 healthy individuals and analyze blood molecular profiles including proteomics, transcriptomics, lipidomics, metabolomics, autoantibodies andimmune cell profiling, complementedwith gut microbiota composition and routine clinical chemistry. Overall, our results show high variation between individuals across different molecular readouts, while the intra-individual baseline variation is low. The analyses show that each individual has a unique and stable plasma protein profile throughout the study period and that many individuals also show distinct profiles with regards to the other omics datasets, with strong underlying connections between the blood proteome and the clinical chemistry parameters. In conclusion, the results support an individual-based definition of health and show that comprehensive omics profiling in a longitudinal manner is a path forward for precision medicine.
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| 15. |
- Alijagic, Andi, 1992-, et al.
(author)
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Characteristics and health risks of the inhalable fraction of metal additive manufacturing powders
- 2024
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In: Nano Select. - : Wiley-VCH Verlagsgesellschaft. - 2688-4011. ; 5:4
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Journal article (peer-reviewed)abstract
- Metal additive manufacturing (AM) is gaining traction but raises worker health concerns due to micron-sized powders, including fine inhalable particles. This study explored particle and surface characteristics, electrochemical properties, metal release in artificial lysosomal fluid (ALF), and potential toxicity of virgin and sieved virgin Fe-based powders, stainless steel (316L), Fe, and two tooling steels. Virgin particles ranged in size from 1 to 100μm, while sieved particles were within the respirable size range (<5–10μm). Surface oxide composition differed from bulk composition. The Fe powder showed low corrosion resistance and high metal release due to a lack of protective surface oxide. Sieved particles of 316L, Fe, and one tooling steel released more metals into ALF than virgin particles, with the opposite was observed for the other tooling steel. Sieved particles had no notable impact on cell viability or micronuclei formation in human bronchial epithelial cells. Inflammatory response in human macrophages was generally low, except for the Fe powder and one tooling steel, which induced increased interleukin-8 (IL-8/CXCL-8) and monocyte chemoattractant protein-1 (MCP-1/CCL-2) secretion. This study underscores distinctions between virgin and sieved Fe-based powders and suggests relatively low acute toxicity.
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| 16. |
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| 17. |
- Bagheri, Alireza, et al.
(author)
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A 16 × 16 45° Slant-Polarized Gapwaveguide Phased Array With 65-dBm EIRP at 28 GHz
- 2023
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In: IEEE Transactions on Antennas and Propagation. - 0018-926X .- 1558-2221. ; 71:2, s. 1319-1329
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Journal article (peer-reviewed)abstract
- A high equivalent isotropic radiated power (EIRP) active phased array antenna is proposed for fifth-generation (5G) communication systems at 28 GHz. The numerical design, measurements of a fabricated prototype, and performance analysis are presented. The antenna design is based on the gapwaveguide technology and consists of $16 \times 16$ single 45° slant-polarized elements. The proposed design uses a low complexity printed circuit board (PCB) structure with only six layers, i.e., a half of the existing wideband solutions. The array antenna incorporates up/downconverter integrated circuits (UDCs) and $1 \times 4$ transceiver beamformer integrated circuits (BFICs). Moreover, a compact and highly efficient transition at the end of each channel of the BFICs has been designed to interconnect the antenna elements with the PCB. The antenna's front-end loss, which includes the feed line, mismatch, and ohmic losses, is only 1.3 dB. The array covers the scanning range of ±60° in the azimuth plane and ±10° in the elevation plane. The $S_{11} < -10$ dB frequency bandwidth is from 26.5 to 29.5 GHz. The maximum EIRP of the antenna is 65.5 dBm at saturation point. The presented design offers a compact, robust, and low-loss performance solution meeting the high transmission power requirements of 5G applications.
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| 18. |
- Bagheri, Alireza, et al.
(author)
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Micro strip to Ridge Gap Waveguide Transition for 28 GHz Steerable Slot Array Antennas
- 2020
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In: 2020 14TH EUROPEAN CONFERENCE ON ANTENNAS AND PROPAGATION (EUCAP 2020). - 2164-3342. - 9788831299008
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Conference paper (peer-reviewed)abstract
- In this paper three types of contactless vertical transitions from microstrip to double ridge waveguide are presented. The designs are compact in size and robust, with improved isolation by employing a pin structure, making them ideal for SG mmWave phased arrays. All transitions cover the 26.5 - 29.5 GHz band, their dimensions are less than half a wavelength in pitch and have insertion losses less than 0.6 dB. The three designs apply different matching strategies and offer a trade-off between bandwidth and PCB areas. Finally, the behavior within an array configuration is analyzed.
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| 19. |
- Bagheri, Alireza, et al.
(author)
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TX Beamforming EVM Performance of a 65 dBm-EIRP Slant-Polarized Gapwaveguide Phased Array at 28 GHz
- 2023
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In: 17th European Conference on Antennas and Propagation, EuCAP 2023.
-
Conference paper (peer-reviewed)abstract
- The transmit error vector magnitude (EVM) performance measurements of a slant-polarized 28 GHz gapwaveguide-based phased array is analyzed in this paper. The performance is studied by using a large range of signals, with varied powers, symbol rates, and quadrature amplitude modulation (QAM) orders. The measurements have been conducted for four different scanning angles. A non-standardized over-the-air (OTA) link between the phased array and the receiver in a laboratory environment is used. The results show that the phased array antenna supports a 31 dB effective isotropic radiated power (EIRP) dynamic range at a maximum 2% EVM when transmitting a 64QAM modulated signal with 250 MS/s symbol rate at all scanning directions.
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| 20. |
- Baresel, Christian, et al.
(author)
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PFAS – how can Swedish wastewater treatment plants meet the challenge? Compilation of knowledge and guidance for water/wastewater actors regarding PFAS
- 2023
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Reports (other academic/artistic)abstract
- Per- and Polyfluoroalkyl Substances (PFAS) are everywhere around us in society, found in commercial and industrial products, the atmosphere, waste, water (waste, surface, drinking, and ground), soil, plants, animals, and even in our bodies. The use and spread of PFAS is a global societal challenge, affecting even the most remote places on Earth. One of the reasons why PFAS has been an attractive component in many products and industrial applications is their extreme chemical and thermal stability. However, these same properties allow for the persistence of PFAS in the environment, whereby even low PFAS emissions over time can be accumulated and pose a high risk of negative health and environmental effects. Today there are thousands of known and unknown PFAS with widely varying properties and toxicity, which makes both risk assessments and management of this growing environmental problem difficult. According to a national mass balance for PFAS emissions from products and atmospheric deposition are the major sources of PFAS in Sweden.The amount of PFAS which are environmentally dispersed via wastewater and sewage sludge can be considered a minor part. However, due to the persistence of PFAS, measures to minimize their addition via these pathways may be necessary to reduce the total environmental load. Due to their toxicity and persistence, PFAS have recently become heavily regulated, with many regulatory agencies lowering the accepted PFAS environmental level ranges. Many PFAS have already been banned in Sweden or the EU and assessment grounds or action limits have been defined for various PFAS (e.g., surface water bodies, groundwater and drinking water) to initiate measures to reduce the spread of the substances. Already announced and stricter regulations will further increase the need for measures to minimize human exposure to PFAS and their dispersal in the environment.Regardless of which measures are implemented, PFAS will remain in the environment for a long time, even if a global ban of the chemicals is implemented. Long-term management of PFAS is thus necessary to removal from the cycle gradually. The focus of mitigation actions should primarily be on heavily contaminated land and landfill leachate. The review of existing data from Swedish wastewater treatment plants WWTP and receiving recipients shows that today’s treatment processes do not remove PFAS. At some WWTP, however, an effective separation of certain PFAS is observed, which should be investigated further. Perfluorooctanoic sulfonic acid (PFOS) levels in many of the investigated inland surface waters receiving effluent from treatment plants and PFAS from other sources/pathways exceed existing limits. In many cases, however, analysis limitations prevent an assessment.Ongoing activities around various treatment and destruction techniques for PFAS show that there are currently no techniques that achieve a far-reaching PFAS removal from municipal wastewater without significant resource consumption and related costs. For the continued use of sludge as a fertilizer, upstream mitigation is needed, with e.g., disconnection or treatment of PFAS-contaminated leachate. However, several ongoing projects indicate that a certain part of PFAS in wastewater can be removed as a side-effect of advanced treatment for pharmaceutical removal. This report provides guidance to stakeholders on how the PFAS problem can be tackled. It also demonstrates the need to improve and spread PFAS knowledge, particularly those involved with PFAS measurement data, treatment techniques and PFAS in sludge.
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| 21. |
- Baresel, Christian, et al.
(author)
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PFAS – Hur kan svenska avloppsreningsverk möta denna utmaning? : Kunskapssammanställning och vägledning för VA-aktörer kring PFAS
- 2022
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Reports (other academic/artistic)abstract
- PFAS (Per- och polyfluorerade alkylsubstanser) finns överallt omkring oss i samhället; i produkter, atmosfären, avfall, avloppsvatten, ytvatten, dricksvatten, grundvatten, mark, växter, djur och i våra kroppar. Användning och spridning av PFAS är en global samhällsutmaning och inte ens de mest avlägsna platserna på jorden är längre opåverkade av PFAS-ämnen. En av anledningarna till att PFAS har varit attraktiva i många produkter och industriella applikationer är ämnenas extrema kemiska och termiska stabilitet. Samma egenskaper skapar dock utmaningar i miljön då persistensen av PFAS medför att även låga utsläpp över tid kan anrikas i olika miljöer med stor risk för negativa hälso- och miljöeffekter. Det finns idag tusentals kända och okända PFAS med väldigt varierande egenskaper och toxicitet, vilket försvårar både en riskbedömning och hantering av detta växande miljöproblem.En nationell massbalans för PFAS visar att emissioner från produkter och atmosfärisk deposition är de största källorna till PFAS-spridning i miljön i Sverige. De mängder som sprids till miljön via avloppsvatten och avloppsslam kan anses utgöra en mindre del. På grund av PFAS-ämnenas persistens kan dock åtgärder för att minimera tillskottet via dessa spridningsvägar vara aktuellt för att nationellt minska den totala miljöbelastningen.Med tanke på toxiciteten och persistensen hos PFAS har användningen av kemikalierna reglerats kraftigt under senare tid och vilka halter som anses acceptabla i miljön har sänkts. Många PFAS har redan förbjudits i Sverige eller EU och bedömningsgrunder eller åtgärdsgränser har definierats för olika PFAS i t.ex. ytvattenförekomster, grundvatten och dricksvatten för att initiera åtgärder för att minska spridningen av ämnena. Redan aviserade och kommande strängare regelverk kommer ytterligare öka behovet av åtgärder för att minimera mänsklig exponering av PFAS och deras spridning i miljön. Oavsett vilka åtgärder som genomförs kommer dock PFAS finnas kvar länge i miljön, även vid ett globalt totalförbud. En långsiktig hantering av PFAS är således nödvändig med en successiv avskiljning från kretsloppet. Fokus för avskiljning från kretsloppet bör framförallt ligga på kraftigt förorenade marker och deponilakvatten.Genomgången av befintliga data vid svenska avloppsreningsverk och mottagande recipienter visar tydligt att dagens reningsprocesser inte avskiljer PFAS. Vid några avloppsreningsverk observeras dock en effektiv avskiljning av vissa PFAS vilket bör undersökas vidare. PFOS-halter i mottagande recipienter överskrider i de flesta fall som tillåter en bedömning befintliga gränsvärden. I många andra fall förhindrar brister i analysen en bedömning på grund av för höga rapporteringsgränser.Pågående aktiviteter kring olika renings- och destruktionstekniker för PFAS visar att det idag inte finns tekniker som åstadkommer en långtgående PFAS-reduktion från kommunalt avloppsvatten utan betydande resursförbrukning och kostnader. För en fortsatt användning av slam som gödsel behöver uppströmsarbete, med bl.a. bortkoppling eller rening av PFAS-förorenat lakvatten intensifieras. Flera pågående pilottester tyder dock på att en viss del av PFAS i avloppsvatten kan renas bort som en synergieffekt om rätt teknik väljs när ett reningsverk kompletteras med avancerad rening för reduktion av läkemedelsrester eller för en cirkulär vattenhantering. Utöver ett kunskapsunderlag ger även denna rapport en vägledning till VA-aktörer för hur PFAS-problematiken kan angripas. Dessutom visar rapporten på ett stort behov av att förbättra och sprida kunskap kring PFAS med framförallt mätdata och kunskap kring reningstekniker och PFAS i slam för att kunna möta PFAS-utmaningen.
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| 22. |
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| 23. |
- Biermann, Jana, et al.
(author)
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A 17-marker panel for global genomic instability in breast cancer.
- 2020
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In: Genomics. - : Elsevier BV. - 0888-7543 .- 1089-8646. ; 112:2, s. 1151-1161
-
Journal article (peer-reviewed)abstract
- Genomic instability is a hallmark of cancer that plays a pivotal role in breast cancer development and evolution. A number of existing prognostic gene expression signatures for breast cancer are based on proliferation-related genes. Here, we identified a 17-marker panel associated with genome stability. A total of 136 primary breast carcinomas were stratified by genome stability. Matched gene expression profiles showed an innate segregation based on genome stability. We identified a 17-marker panel stratifying the training and validation cohorts into high- and low-risk patients. The 17 genes associated with genomic instability strongly impacted clinical outcome in breast cancer. Pathway analyses determined chromosome organisation, cell cycle regulation, and RNA processing as the underlying biological processes, thereby offering options for drug development and treatment tailoring. Our work supports the applicability of the 17-marker panel to improve clinical outcome prediction for breast cancer patients based on a signature accounting for genomic instability.
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| 24. |
- Bivehed, Erik, 1990-
(author)
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Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay
- 2023
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Doctoral thesis (other academic/artistic)abstract
- Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. DNA damage is a critical precursor to cancer development, highlighting the need for tests to predict genotoxicity and mutagenicity of various agents, including pharmaceuticals and environmental factors. This thesis focuses on enhancing the single cell gel electrophoresis (comet assay) for assessing primary DNA damage.The work was concentrated around several fundamental aspects of the methodology where a novel statistical approach, Uppsala Comet Data Analysis Strategy (UCDAS), was developed for data evaluation. A proportional odds model tailored to continuous outcomes was used, accommodating the experimental design's hierarchy, large zero values, and avoiding data transformation. A revisit of the formulation of the electrophoresis medium led to the introduction of a low conductive lithium hydroxide-based solution, enabling higher field strengths, significantly reducing runtimes and increasing sensitivity compared to the conventional comet assay. A lot of work was done on the investigation of the pH's impact on DNA integrity, revealing elevated background DNA damage at higher pH levels. Extended unwinding at pH >13, typical of the most commonly used versions of alkaline comet assays, jeopardizes the integrity of DNA, resulting in greater background DNA damage than at lower pH values. The study underscores pH's significance for DNA stability, highlighting risks associated with extremely alkaline conditions.A new method was developed, the Polymerase Assisted DNA Damage Assay (PADDA), to label and quantify single- and double-strand DNA breaks selectively in comet heads and tails after exposure to established DNA-damaging agents. This approach also allowed detection of DNA damage inside comet heads, an ability lacking in traditional comet assays.In conclusion, this research enhances DNA damage assessment methodologies, introducing new statistical innovations, novel electrophoresis mediums, and a novel technique for the selective detection and quantification of single- and double-strand breaks. These advancements deepen our understanding of DNA damage's complexities and underscore the crucial role of pH in influencing DNA stability and its implications for genotoxicity assessment.
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| 25. |
- Björner Brauer, Hanna, et al.
(author)
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Pain Relieving Light - (How) Is it Possible?
- 2024. - 1
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In: IOP Science home Accessibility Help Journals Books Publishing Support Login IOP Conference Series: Earth and Environmental Science. - 1755-1307. ; 1320
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Conference paper (peer-reviewed)abstract
- About 15% of the population suffer from migraines and it is estimated that about 40% of all people with migraines would benefit from preventive treatment, but only 3-13% use it. Migraine is a huge burden for society and individuals. Migraines can be intensified by light, and some patients need to stay in a dark room until the attack is over. People with this type of photosensitivity show a clear preference for light color, specifically green, which has been shown to be more comfortable and even pain relieving in some cases. We will present a feasibility study with the aim of preparing a series of experiments to investigate if regular short-term green-light-exposure can prevent migraines. We will present findings from the literature, developed light equipment, and plans for future testing of migraine friendly light solutions.
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| 26. |
- Blomberg, Sara, et al.
(author)
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Bridging the Pressure Gap in CO Oxidation
- 2021
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In: ACS Catalysis. - : American Chemical Society (ACS). - 2155-5435. ; 11:15, s. 9128-9135
-
Journal article (peer-reviewed)abstract
- Performing fundamental operando catalysis studies under realistic conditions is a key to further develop and increase the efficiency of industrial catalysts. Operando X-ray photoelectron spectroscopy (XPS) experiments have been limited to pressures, and the relevance for industrial applications has been questioned. Herein, we report on the CO oxidation experiment on Pd(100) performed at a total pressure of 1 bar using XPS. We investigate the light-off regime and the surface chemical composition at the atomistic level in the highly active phase. Furthermore, the observed gas-phase photoemission peaks of CO2, CO, and O2 indicate that the kinetics of the reaction during the light-off regime can be followed operando, and by studying the reaction rate of the reaction, the activation energy is calculated. The reaction was preceded by an in situ oxidation study in 7% O2 in He and a total pressure of 70 mbar to confirm the surface sensitivity and assignment of the oxygen-induced photoemission peaks. However, oxygen-induced photoemission peaks were not observed during the reaction studies, but instead, a metallic Pd phase is present in the highly active regime under the conditions applied. The novel XPS setup utilizes hard X-rays to enable high-pressure studies, combined with a grazing incident angle to increase the surface sensitivity of the measurement. Our findings demonstrate the possibilities of achieving chemical information of the catalyst, operando, on an atomistic level, under industrially relevant conditions.
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| 27. |
- Bornäs, Hanna, 1983-
(author)
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Subjects of Violence : On Gender and Recognition in Young Men’s Violence Against Women
- 2022
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Doctoral thesis (other academic/artistic)abstract
- The dissertation concerns young men’s violence against women partners. It is based on in-depth qualitative interviews with nine men who have been violent against women partners in their youth, and an additional interview with the mother of one of the young men. The method is informed by Hollway and Jefferson’s psychosocial methodology and Hydén’s teller-focused interview approach. The interviewees’ stories of violence are analysed combining psychoanalytic theories of intersubjectivity with an attention to discourses. The aim of the dissertation is to explore men’s experiences of being violent against women partners in youth and to investigate the gendered intersubjective dynamics of young men’s violence against women partners. Jessica Benjamin’s theories on gender and recognition are central to the analyses, and other feminist, psychoanalytic and psychosocial theories are used in the dissertation’s analysis of the men’s stories of violence. The study highlights the role of early relationships, gendered identifications, recognition, and discourses of masculinity and sexuality in using and desisting from violence. Men’s identifications and disidentifications with violent father figures are particularly significant, as are relationships with male peers in youth and the men’s (denied) vulnerabilities. The temporality and liminality of youth are also explored, as the first romantic relationship poses particular challenges to young men who have been exposed to violence and abuse from a young age, or who lack parental support. The time of youth figures as a porous boundary of old and new dependencies, hierarchies and relationship patterns. It is shown how the men’s definitions of violence are also shifting, and the particular nexus of love and aggression within relationships is thus highlighted. Violent situations are demonstrated to denote a breakdown in mutual recognition, which, using Benjamin’s notions, takes the form of oneness – denying difference and alterity – or twoness – over-emphasizing difference and complementarity. In line with Donald Winnicott, these processes of non-recognition involve failed destruction and survival – the inability on the part of the men to tolerate their partners’ acts of negation without retaliating. Desisting from violence consequently involves striving towards an ideal of thirdness or reciprocal recognition.Another central finding is the prevailing experiences of exerting sexual coercion in youth. In situations of pressurized sex, the men fail to recognize the sexual subjectivity of the woman other. The change in the interviewee’s experiences troubles a linear temporality, and by using the psychoanalytic notion of afterwardsness – it is shown how the men become retroactive perpetrators, which reorganizes their embodied and affective memories and subjectivities. By stressing the nonlinear qualities of temporality and memories, this dissertation destabilizes the idea of childhood and youth, pointing to the unfinished and (re-)constructed nature of these life phases, while simultaneously arguing for their vital importance and ‘real’ influence in the lives of subjects. This is thus a contribution to youth studies as well as an argument for broadening the conception of the youth subject.
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| 28. |
- Bäckberg, Matilda, et al.
(author)
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Using in vitro receptor activity studies of synthetic cannabinoids to support the risk assessment of new psychoactive substances – A Swedish strategy to protect public health from harm
- 2023
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In: Forensic Science International. - : Elsevier Ireland Ltd. - 0379-0738 .- 1872-6283. ; 348
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Journal article (peer-reviewed)abstract
- In the past 15 years, close to 1000 of new psychoactive substances (NPS) have been reported in Europe and globally. At the time of identification, data on safety, toxicity and carcinogenic potential of many NPS are not available or very limited. To work more efficiently, a strategy and collaboration between the Public Health Agency of Sweden (PHAS) and the National Board of Forensic Medicine was established involving in vitro receptor activity assays to demonstrate neurological activity of NPS. This report summarizes the first results on the synthetic cannabinoid receptor agonists (SCRAs), and subsequent actions taken by PHAS. A total of 18 potential SCRAs were selected by PHAS for in vitro pharmacological characterization. 17 compounds could be acquired and investigated for their activity on the human cannabinoid-1 (CB1) receptors expressed together with the AequoScreen system in CHO-K1 cells. Dose-response curves were established using eight different concentrations in triplicates at three occasions with JWH-018 as reference. For the MDMB-4en-PINACA, MMB-022, ACHMINACA, ADB-BUTINACA, 5F-CUMYL-PeGACLONE, 5C-AKB48, NM-2201, 5F-CUMYL-PINACA, JWH-022, 5Cl-AB-PINACA, MPhP-2201, 5F-AKB57 the half maximal effective concentration values ranged from 2.2 nM (5F-CUMYL-PINACA) to 171 nM (MMB-022). EG-018 and 3,5-AB-CHMFUPPYCA were none-active. The results contributed to 14 of these compounds being scheduled as narcotics in Sweden. In conclusion, many of the emerging SCRAs are potent activators of the CB1 receptor in vitro, although some lack activity or are partial agonists. The new strategy proved useful when data on psychoactive effects of the SCRAs under investigation were not available or limited. © 2023 The Authors
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| 29. |
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| 30. |
- Candefjord, Stefan, et al.
(author)
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Video support for prehospital stroke consultation: implications for system design and clinical implementation from prehospital simulations
- 2024
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In: BMC Medical Informatics and Decision Making. - 1472-6947. ; 24:1
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Journal article (peer-reviewed)abstract
- BackgroundVideo consultations between hospital-based neurologists and Emergency Medical Services (EMS) have potential to increase precision of decisions regarding stroke patient assessment, management and transport. In this study we explored the use of real-time video streaming for neurologist–EMS consultation from the ambulance, using highly realistic full-scale prehospital simulations including role-play between on-scene EMS teams, simulated patients (actors), and neurologists specialized in stroke and reperfusion located at the remote regional stroke center.MethodsVideo streams from three angles were used for collaborative assessment of stroke using the National Institutes of Health Stroke Scale (NIHSS) to assess symptoms affecting patient’s legs, arms, language, and facial expressions. The aim of the assessment was to determine appropriate management and transport destination based on the combination of geographical location and severity of stroke symptoms. Two realistic patient scenarios were created, with severe and moderate stroke symptoms, respectively. Each scenario was simulated using a neurologist acting as stroke patient and an ambulance team performing patient assessment. Four ambulance teams with two nurses each all performed both scenarios, for a total of eight cases. All scenarios were video recorded using handheld and fixed cameras. The audio from the video consultations was transcribed. Each team participated in a semi-structured interview, and neurologists and actors were also interviewed. Interviews were audio recorded and transcribed.ResultsAnalysis of video-recordings and post-interviews (n = 7) show a more thorough prehospital patient assessment, but longer total on-scene time, compared to a baseline scenario not using video consultation. Both ambulance nurses and neurologists deem that video consultation has potential to provide improved precision of assessment of stroke patients. Interviews verify the system design effectiveness and suggest minor modifications.ConclusionsThe results indicate potential patient benefit based on a more effective assessment of the patient’s condition, which could lead to increased precision in decisions and more patients receiving optimal care. The findings outline requirements for pilot implementation and future clinical tests.
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| 31. |
- Cappellini, Francesca, et al.
(author)
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Dry Generation of CeO2 Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface-Dosimetry Considerations and Comparison to Submerged Exposure
- 2020
-
In: Nanomaterials. - : MDPI AG. - 2079-4991. ; 10:4
-
Journal article (peer-reviewed)abstract
- Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO2 NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1 beta release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO2 NPs was generated by using the PreciseInhale (R) system, and NPs were deposited on the co-culture using XposeALI (R). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1 beta, IL-6, TNF alpha, MCP-1) were observed after exposure of the co-culture in ALI (max 5 mu g/cm(2)) or submerged (max 22 mu g/cm(2)) conditions. In contrast, CeO2 NPs cause clear IL-1 beta release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions.
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| 32. |
- Ekelund, Malin, et al.
(author)
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Does Expertise Reduce Rates of Inattentional Blindness? A Meta-Analysis
- 2022
-
In: Perception. - : SAGE Publications. - 0301-0066 .- 1468-4233. ; 51:2, s. 131-147
-
Journal article (peer-reviewed)abstract
- Inattentional blindness occurs when one fails to notice a fully visible stimulus because one's attention is on another task. Researchers have suggested that expertise at this other task should reduce rates of inattentional blindness. However, research on the topic has produced mixed findings. To gain clarity on the issue, we meta-analyzed the extant studies (K = 14; N = 1153). On average, experts showed only a slight reduction in rates of inattentional blindness: 62% of novices experienced inattentional blindness compared to 56% of experts, weighted odds ratio = 1.33, 95% CI [0.78, 2.28]. The relevance of the stimuli to the experts’ domain of expertise showed no notable moderating effects. The low number of the included studies, and the small sample sizes of the original studies, weaken our conclusions. Nonetheless, when taken together, the available evidence provides little support for any reliable influence of expertise on rates of inattentional blindness. © The Author(s) 2022.
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| 33. |
- Elihn, Karine, et al.
(author)
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Air quality impacts of a large waste fire in Stockholm, Sweden
- 2023
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In: Atmospheric Environment. - : Elsevier BV. - 1352-2310 .- 1873-2844. ; 315
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Journal article (peer-reviewed)abstract
- Fires in waste facilities are a common occurrence. Since many waste facilities are located adjacent to densely populated areas, these fires could potentially expose large populations to the emitted pollutants. However, at the moment there are only few field studies investigating the impact of waste fire emissions on air quality since the unpredictable nature of these events makes them challenging to capture. This study investigated the impact of a large and persistent un-prescribed fire in a waste storage facility in Stockholm county, Sweden, on the local air quality of two residential areas in close proximity to the fire. In-situ measurements of particulate matter, black carbon and nitrogen oxide concentrations were conducted both during open burning and after the fire was fully covered. In addition, filter samples were collected for offline analysis of organic composition, metal content and toxicity. Strongly increased concentrations of PM10, PM2.5 and black carbon were found during the open burning period, especially when the wind was coming from the direction of the fire. In addition, elevated concentrations of particulate heavy metals and polycyclic aromatic hydrocarbons were observed in the air during the open burning period. These results show that waste fires can have a strong impact on the air quality of nearby residential areas.
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| 34. |
- Engqvist, Hanna, 1985, et al.
(author)
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Integrative genomics approach identifies molecular features associated with early-stage ovarian carcinoma histotypes.
- 2020
-
In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
-
Journal article (peer-reviewed)abstract
- Ovarian cancer comprises multiple subtypes (clear-cell (CCC), endometrioid (EC), high-grade serous (HGSC), low-grade serous (LGSC), and mucinous carcinomas (MC)) with differing molecular and clinical behavior. However, robust histotype-specific biomarkers for clinical use have yet to be identified. Here, we utilized a multi-omics approach to identify novel histotype-specific genetic markers associated with ovarian carcinoma histotypes (CCC, EC, HGSC, and MC) using DNA methylation, DNA copy number alteration and RNA sequencing data for 96 primary invasive early-stage (stage I and II) ovarian carcinomas. More specifically, the DNA methylation analysis revealed hypermethylation for CCC in comparison with the other histotypes. Moreover, copy number imbalances and novel chromothripsis-like rearrangements (n=64) were identified in ovarian carcinoma, with the highest number of chromothripsis-like patterns in HGSC. For the 1000 most variable transcripts, underexpression was most prominent for all histotypes in comparison with normal ovarian samples. Overall, the integrative approach identified 46 putative oncogenes (overexpressed, hypomethylated and DNA gain) and three putative tumor suppressor genes (underexpressed, hypermethylated and DNA loss) when comparing the different histotypes. In conclusion, the current study provides novel insights into molecular features associated with early-stage ovarian carcinoma that may improve patient stratification and subclassification of the histotypes.
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| 35. |
- Engqvist, Hanna, 1985, et al.
(author)
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Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry
- 2020
-
In: Frontiers in Oncology. - : Frontiers Media SA. - 2234-943X. ; 10
-
Journal article (peer-reviewed)abstract
- Early-stage (I and II) ovarian carcinoma patients generally have good prognosis. Yet, some patients die earlier than expected. Thus, it is important to stratify early-stage patients into risk groups to identify those in need of more aggressive treatment regimens. The prognostic value of 29 histotype-specific biomarkers identified using RNA sequencing was evaluated for early-stage clear-cell (CCC), endometrioid (EC) and mucinous (MC) ovarian carcinomas (n = 112) using immunohistochemistry on tissue microarrays. Biomarkers with prognostic significance were further evaluated in an external ovarian carcinoma data set using the web-based Kaplan-Meier plotter tool. Here, we provide evidence of aberrant protein expression patterns and prognostic significance of 17 novel histotype-specific prognostic biomarkers [10 for CCC (ARPC2, CCT5, GNB1, KCTD10, NUP155, RPL13A, RPL37, SETD3, SMYD2, TRIO), three for EC (CECR1, KIF26B, PIK3CA), and four for MC (CHEK1, FOXM1, KIF23, PARPBP)], suggesting biological heterogeneity within the histotypes. Combined predictive models comprising the protein expression status of the validated CCC, EC and MC biomarkers together with established clinical markers (age, stage, CA125, ploidy) improved the predictive power in comparison with models containing established clinical markers alone, further strengthening the importance of the biomarkers in ovarian carcinoma. Further, even improved predictive powers were demonstrated when combining these models with our previously identified prognostic biomarkers PITHD1 (CCC) and GPR158 (MC). Moreover, the proteins demonstrated improved risk prediction of CCC-, EC-, and MC-associated ovarian carcinoma survival. The novel histotype-specific prognostic biomarkers may not only improve prognostication and patient stratification of early-stage ovarian carcinomas, but may also guide future clinical therapy decisions.
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| 36. |
- Ericsson, Stina, 1972, et al.
(author)
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Att analysera interaktion
- 2023
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Book (other academic/artistic)abstract
- Snart sagt alla situationer en människa befinner sig i präglas av interaktion. Människor pratar med varandra, rör sig genom ett rum, utbyter blickar, lär sig saker, arbetar, skämtar, håller i föremål ... Utmärkande för oss människor är nämligen att vi får saker och ting gjorda med vårt språk och våra kroppsliga resurser. Genom sådana handlingar påverkar vi vår omvärld och våra medmänniskor - och vi gör det tillsammans med dem. Det är studiet av denna mänskliga interaktion som boken handlar om. I första delen introduceras forskningsfältet interaktionsanalys och de arbetssätt, redskap och etiska ställningstaganden som hör till fältet. Den andra delen innehåller forskningsstudier som visar på bredden inom fältet och som pedagogiskt leder läsaren genom hela analysarbetet. Till boken hör även ett videomaterial som illustrerar några av bokens exempel, och som är tillgängligt för läsarens egna studier. Att analysera interaktion är avsedd för utbildningar inom språk vetenskap och angränsande ämnen som sociologi, utbildningsvetenskap och kommunikation.
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| 37. |
- Eriksson, Hanna, et al.
(author)
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Acute symptomatic seizures and epilepsy after mechanical thrombectomy
- 2020
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In: Epilepsy and Behavior. - : Elsevier BV. - 1525-5050 .- 1525-5069. ; 104
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Journal article (peer-reviewed)abstract
- Purpose: The purpose of this study was to assess the incidence of acute symptomatic seizures and poststroke epilepsy (PSE) in a well-characterized cohort of patients treated with mechanical thrombectomy. In addition, we aimed to describe the dynamics of blood markers of brain injury in patients that developed PSE. Methods: Participants of the prospective AnStroke Trial of anesthesia method during mechanical thrombectomy were included and acute symptomatic seizures and PSE ascertained by medical records review. Blood markers neurofilament light (NFL), tau, glial fibrillary acidic protein (GFAP), S100 calcium-binding protein B (S100B), and neuron-specific enolase (NSE) were assessed. Results: A total of 90 patients with acute anterior ischemic stroke were included. Median National Institutes of Health Stroke Scale (NIHSS) at admission to hospital was 18 (IQR 15–22). Recanalization was achieved in 90%. No patients had epilepsy prior to the ischemic stroke. Four patients (4.4%) had acute symptomatic seizures and four patients (4.4%) developed PSE during the follow-up time (to death or last medical records review) of 0–4.5 years (median follow-up 1070 days IQR 777–1306), resulting in a two-year estimated PSE risk of 5.3% (95%CI: 0.2–10.4%). Blood markers of brain injury (NFL, tau, GFAP, S100B, and NSE) were generally above the cohort median in patients that developed PSE. Conclusions: The incidence of PSE after mechanical thrombectomy was low in our cohort. All blood biomarkers displayed interesting sensitivity and specificity. However, the number of PSE cases was small and more studies are needed on risk factors for PSE after mechanical thrombectomy. The potential of blood markers of brain injury markers to contribute to assessment of PSE risk should be explored further. This article is part of the Special Issue "Seizures & Stroke".
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| 38. |
- Fernlund, Eva, et al.
(author)
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Hereditary hypertrophic cardiomyopathy in children and young adults—The value of reevaluating and expanding gene panel analyses
- 2020
-
In: Genes. - : MDPI AG. - 2073-4425. ; 11:12
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Journal article (peer-reviewed)abstract
- Introduction: Sudden cardiac death (SCD) and early onset cardiomyopathy (CM) in the young will always lead to suspicion of an underlying genetic disorder. Incited by the rapid advances in genetic testing for disease we have revisited families, which previously tested “gene-negative” for familial predominantly pediatric CM, in hopes of finding a causative gene variant. Methods: 10 different families with non-syndromic pediatric CM or hypertrophic cardiomyopathy (HCM) with severe disease progression and/or heredity for HCM/CM related SCD with “gene-negative” results were included. The index patient underwent genetic testing with a recently updated gene panel for CM and SCD. In case of failure to detect a pathogenic variant in a relevant gene, the index patient and both parents underwent clinical (i.e., partial) exome sequencing (trio-exome) in order to catch pathogenic variants linked to the disease in genes that were not included in the CM panel. Results: The mean age at clinical presentation of the 10 index cases was 12.5 years (boys 13.4 years, n = 8; girls 9 years, n = 2) and the family history burden was 33 HCM/CM cases including 9 HCM-related SCD and one heart transplantation. In 5 (50%) families we identified a genetic variant classified as pathogenic or likely pathogenic, in accordance with the American College of Medical Genetics and Genomics (ACMG) criteria, in MYH7 (n = 2), RBM20, ALPK3, and PGM1, respectively, and genetic variants of unknown significance (VUS) segregating with the disease in an additional 3 (30%) families, in MYBPC3, ABCC9, and FLNC, respectively. Conclusion: Our results show the importance of renewed thorough clinical assessment and the necessity to challenge previous genetic test results with more comprehensive updated gene panels or exome sequencing if the initial test failed to identify a causative gene for early onset CM or SCD in children. In pediatric cardiomyopathy cases when the gene panel still fails to detect a causative variant, a trio exome sequencing strategy might resolve some unexplained cases, especially if a multisystemic condition is clinically missed.
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| 39. |
- Gericke, Sabrina Maria, et al.
(author)
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In Situ H2 Reduction of Al2O3-Supported Ni- and Mo-Based Catalysts
- 2022
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In: Catalysts. - : MDPI. - 2073-4344. ; 12:7
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Journal article (peer-reviewed)abstract
- Nickel (Ni)-promoted Molybdenum (Mo)-based catalysts are used for hydrotreatment processes in the chemical industry where the catalysts are exposed to high-pressure H2 at elevated temperature. In this environment, the catalyst transforms into the active phase, which involves the reduction of the oxide. Here, we report on the first in situ study on the reduction of alumina supported Ni- and Mo-based catalysts in 1 mbar H2 using ambient-pressure X-ray photoelectron spectroscopy (APXPS). The study confirms that mixing Ni and Mo lowers the reduction temperature of both Ni- and Mo-oxide as compared to the monometallic catalysts and shows that the MoO3 reduction starts at a lower temperature than the reduction of NiO in NiMo/Al2O3 catalysts. Additionally, the reduction of Ni and Mo foil was directly compared to the reduction of the Al2O3-supported catalysts and it was observed that the reduction of the supported catalysts is more gradual than the reduction of the foils, indicating a strong interaction between the Ni/Mo and the alumina support. © 2022 by the authors.
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| 40. |
- Gliga, Anda R., et al.
(author)
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Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor signaling in immune-competent human cell lines
- 2020
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In: Nanoscale Advances. - : ROYAL SOC CHEMISTRY. - 2516-0230. ; 2:2, s. 648-658
-
Journal article (peer-reviewed)abstract
- Silver (Ag) nanoparticles are commonly used in consumer products due to their antimicrobial properties. Here we studied the impact of Ag nanoparticles on immune responses by using cell lines of monocyte/macrophage and lung epithelial cell origin, respectively. Short-term experiments (24 h) showed that Ag nanoparticles reduced the lipopolysaccharide (LPS)-induced secretion of pro-inflammatory cytokines in THP-1 cells under serum-free conditions. ICP-MS analysis revealed that cellular uptake of Ag was higher under these conditions. Long-term exposure (up to 6 weeks) of BEAS-2B cells to Ag nanoparticles also suppressed pro-inflammatory cytokine production following a brief challenge with LPS. Experiments using reporter cells revealed that Ag nanoparticles as well as AgNO3 inhibited LPS-triggered Toll-like receptor (TLR) signaling. Furthermore, RNA-sequencing of BEAS-2B cells indicated that Ag nanoparticles affected TLR signaling pathways. In conclusion, Ag nanoparticles reduced the secretion of pro-inflammatory cytokines in response to LPS, likely as a result of the release of silver ions leading to an interference with TLR signaling. This could have implications for the use of Ag nanoparticles as antibacterial agents. Further in vivo studies are warranted to study this.
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| 41. |
- Grassi, Lorenzo, et al.
(author)
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3D Finite Element Models Reconstructed From 2D Dual-Energy X-Ray Absorptiometry (DXA) Images Improve Hip Fracture Prediction Compared to Areal BMD in Osteoporotic Fractures in Men (MrOS) Sweden Cohort
- 2023
-
In: Journal of Bone and Mineral Research. - : John Wiley & Sons. - 0884-0431 .- 1523-4681. ; 38:9, s. 1258-1267
-
Journal article (peer-reviewed)abstract
- Bone strength is an important contributor to fracture risk. Areal bone mineral density (aBMD) derived from dual-energy X-ray absorptiometry (DXA) is used as a surrogate for bone strength in fracture risk prediction tools. 3D finite element (FE) models predict bone strength better than aBMD, but their clinical use is limited by the need for 3D computed tomography and lack of automation. We have earlier developed amethod to reconstruct the 3D hip anatomy froma 2D DXA image, followed by subject-specific FE-based prediction of proximal femoral strength. In the current study, we aim to evaluate the method's ability to predict incident hip fractures in a populationbased cohort (Osteoporotic Fractures in Men [MrOS] Sweden). We defined two subcohorts: (i) hip fracture cases and controls cohort: 120men with a hip fracture (<10 years frombaseline) and two controls to each hip fracture case, matched by age, height, and body mass index; and (ii) fallers cohort: 86men who had fallen the year before their hip DXA scan was acquired, 15 of which sustained a hip fracture during the following 10 years. For each participant, we reconstructed the 3D hip anatomy and predicted proximal femoral strength in 10 sideways fall configurations using FE analysis. The FE-predicted proximal femoral strength was a better predictor of incident hip fractures than aBMD for both hip fracture cases and controls (difference in area under the receiver operating characteristics curve, Delta AUROC = 0.06) and fallers (Delta AUROC = 0.22) cohorts. This is the first time that FE models outperformed aBMD in predicting incident hip fractures in a population-based prospectively followed cohort based on 3D FE models obtained from a 2D DXA scan. Our approach has potential to notably improve the accuracy of fracture risk predictions in a clinically feasible manner (only one single DXA image is needed) and without additional costs compared to the current clinical approach.
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| 42. |
- Grassi, Lorenzo, et al.
(author)
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3d Finite Element Models Reconstructed From 2d Dxa Images Improve Hip Fracture Prediction Compared to Areal Bmd in Mros Sweden Cohort
- 2023
-
In: Journal of Bone and Mineral Research. - 1523-4681. ; 38:9, s. 1258-1267
-
Journal article (peer-reviewed)abstract
- Bone strength is an important contributor to fracture risk. Areal bone mineral density (aBMD) derived from dual-energy X-ray absorptiometry (DXA) is used as a surrogate for bone strength in fracture risk prediction tools. 3D finite element (FE) models predict bone strength better than aBMD, but their clinical use is limited by the need for 3D computed tomography and lack of automation. We have earlier developed a method to reconstruct the 3D hip anatomy from a 2D DXA image, followed by subject-specific FE-based prediction of proximal femoral strength. In the current study, we aim to evaluate the method's ability to predict incident hip fractures in a population-based cohort (MrOS Sweden). We defined two sub-cohorts: (i) hip fracture cases and controls cohort: 120 men with a hip fracture (<10 years from baseline) and 2 controls to each hip fracture case, matched by age, height, and body mass index; (ii) fallers cohort: 86 men who had fallen the year before their hip DXA scan was acquired, 15 of which sustained a hip fracture during the following 10 years. For each participant, we reconstructed the 3D hip anatomy and predicted proximal femoral strength in 10 sideways fall configurations using FE analysis. The FE-predicted proximal femoral strength was a better predictor of incident hip fractures than aBMD for both hip fracture cases and controls (difference in area under the receiver operating characteristics curve, ΔAUROC = 0.06) and fallers (ΔAUROC = 0.22) cohorts. This is the first time that FE models outperform aBMD in predicting incident hip fractures in a population-based prospectively followed cohort based on 3D FE models obtained from a 2D DXA scan. Our approach has potential to notably improve the accuracy of fracture risk predictions in a clinically feasible manner (only one single DXA image is needed) and without additional costs compared to the current clinical approach.
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| 43. |
- Grundström, Hanna, et al.
(author)
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Previous Trauma Exposure and Its Associations with Fear of Childbirth and Quality of Life among Pregnant Lesbian, Bisexual, Transgender, and Queer People and Their Partners
- 2023
-
In: LGBTQ FAMILY-AN INTERDISCIPLINARY JOURNAL. - : ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD. - 2770-3371 .- 2770-338X. ; 19:2, s. 175-185
-
Journal article (peer-reviewed)abstract
- The primary aim of this study was to determine the prevalence of previous trauma exposure among expectant birth-giving parents and their partners within a LBTQ population. The secondary aims were to compare fear of childbirth (FOC) and quality of life (QoL) in relation to previous trauma exposure in pregnant LBTQ people and their partners. A further aim was to analyze associations between severe FOC and clinical and demographic factors in this population. Data was collected from a Swedish LGBTQ competent antenatal clinic. Trauma-exposed pregnant (n = 32) and non-pregnant (n = 21) individuals and pregnant (n = 48) and non-pregnant (n = 30) individuals without previous trauma experiences responded to instruments measuring FOC (Wijma Delivery Expectancy Questionnaire) and QoL (EuroQol 5 D-index/-visual analogue scale, VAS). Differences between groups were assessed using non-parametric tests. The proportion of trauma exposure was similar among pregnant responders and their partners (40.0% vs. 41.2%). Trauma-exposed pregnant respondents had a significantly higher prevalence of severe FOC compared to the pregnant respondents without previous trauma. Pregnant trauma-exposed respondents scored lower on EQ5D-VAS than pregnant respondents without trauma, as did non-pregnant trauma exposed respondents compared with non-pregnant -respondents without trauma. Furthermore, trauma-exposed non-pregnant respondents scored lower on the EQ5D-index compared to non-pregnant respondents without trauma. Previous trauma was the only clinical and demographic factor that had any significant association withto severe FOC in the regression analysis. In conclusion, our results suggest that previous trauma exposure may contribute to the risk of suffering from severe FOC and lower QoL among LBTQ-identifying prospective parents.
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| 44. |
- Gustafsson, Tanja, PhD student, 1980-, et al.
(author)
-
Nurse assistants´ perceptions of developing person-centred communication
- 2021
-
Conference paper (peer-reviewed)abstract
- Background: Communication is important for nurse assistants (NAs) when caring for older persons. There is limited research about how to improve the communication competence of NAs in home care. The aim was to describe NAs perception on learning in relation to an educational intervention on person-centred communication.Methods: A qualitative descriptive study was conducted. Data consisted of four group interviews, five individual in-depth interviews and written reflection assignments from participants gathered during the web-based intervention. In total 23 NAs at two home care units participated. The data were analysed using a phenomenographic approach. This is a qualitative method for analysis developed from Nordic traditions of phenomenology. Phenomenography aims to describe individual perceptions of a certain phenomenon.Preliminary findings points to the nurse assistants perceiving that the education supported their development of person-centred communication. They pointed out self-reflections as important for their learning as well as to get confirmation on what was already known. The NAs described that the intervention added to their knowledge and skills. Even though, there was more to learn and challenges that remained regarding communication in challenging situations such as caring for persons in end-of-life and supporting their relatives, caring for persons being aggressive or violent.Preliminary implications of research: This study can provide knowledge on participants’ perspective on their learning process, which may be important to consider when conducting educational interventions in home care as well as other health care contexts.
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| 45. |
- Gustafsson, Tanja, PhD student, 1980-, et al.
(author)
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Process evaluation of the ACTION programme : a strategy for implementing person‐centred communication in home care
- 2021
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In: BMC Nursing. - : Springer Nature. - 1472-6955. ; 20:1
-
Journal article (peer-reviewed)abstract
- Background: There is currently a strong emphasis on person-centred care (PCC) and communication; however, little research has been conducted on how to implement person-centred communication in home care settings. Therefore, the ACTION (A person-centred CommunicaTION) programme, which is a web-based education programme focusing on person-centred communication developed for nurse assistants (NAs) providing home care for older persons, was implemented. This paper reports on the process evaluation conducted with the aim to describe and evaluate the implementation of the ACTION programme. Methods: A descriptive design with a mixed method approach was used. Twenty-seven NAs from two units in Sweden were recruited, and 23 of them were offered the educational intervention. Quantitative and qualitative data were collected from multiple sources before, during and after the implementation. Quantitative data were used to analyse demographics, attendance and participation, while qualitative data were used to evaluate experiences of the implementation and contextual factors influencing the implementation. Results: The evaluation showed a high degree of NA participation in the first five education modules, and a decrease in the three remaining modules. Overall, the NAs perceived the web format to be easy to use and appreciated the flexibility and accessibility. The content was described as important. Challenges included time constraints; the heavy workload; and a lack of interaction, space and equipment to complete the programme. Conclusions: The results suggest that web-based education seems to be an appropriate strategy in home care settings; however, areas for improvement were identified. Our findings show that participants appreciated the web-based learning format in terms of accessibility and flexibility, as well as the face-to-face group discussions. The critical importance of organizational support and available resources are highlighted, such as management involvement and local facilitation. In addition, the findings report on the implementation challenges specific to the dynamic home care context. Trial registration: This intervention was implemented with nursing assistants, and the evaluation only involved nursing staff. Patients were not part of this study. According to the ICMJE, registration was not necessary (). © 2021, The Author(s).
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| 46. |
- Gustafsson, Tanja, PhD student, 1980-, et al.
(author)
-
‘Sometimes you need an eye-opener’: A qualitative study on nursing assistants' experiences of developing communication skills through an educational intervention on person-centred communication
- 2023
-
In: Nursing Open. - : John Wiley & Sons. - 2054-1058.
-
Journal article (peer-reviewed)abstract
- AimTo explore nursing assistants' (NAs') experiences of developing communication skills while participating in an educational intervention on person-centred communication.DesignA descriptive qualitative study was conducted.MethodsData were collected from interviews and written assignments before, during and after an educational intervention on person-centred communication targeting NAs in home care services. The data were analysed using a phenomenological approach. A total of 25 NAs participated in the study.ResultsThe findings describe NAs' experiences concerning the communication skills needed for building relationships with older persons and handling emotionally challenging situations. The educational intervention increased their knowledge and awareness of the importance of communication skills and how such skills are developed and refined.
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| 47. |
- Hennlock, Magnus, et al.
(author)
-
Klimatanpassning 2023 - Så långt har Sveriges kommuner kommit
- 2023
-
Reports (other academic/artistic)abstract
- Climate change entails risks for both people and ecosystems, and municipalities need to take measures to protect communities and infrastructure. Vulnerabilities need to be mapped and climate-adapted communities need to be built to cope with rising sea levels, increased risk of torrential rains, erosion, drought, heat waves and landslides in the municipalities. Against this background, Insurance Sweden and IVL Swedish Environmental Research Institute, began a collaboration in 2015 to map the municipalities' strategic climate adaptation work. The purpose of recurring surveys to all of Sweden's 290 municipalities is to get an overview of how climate adaptation work is developing at the local level in Sweden and to identify success factors and challenges that the municipalities are facing. By mapping existing strategies, plans and measures, we can better understand how the municipalities work to meet the challenges of climate change and what efforts need to be made going forward. By highlighting good examples and spreading knowledge, we can also inspire and encourage other municipalities to take measures for climate adaptation. We are publishing the sixth report regarding climate adaptation in Swedish municipalities. As in previous surveys, the questions are based on the tool for climate adaptation work that was launched in connection with the EU's strategy for climate adaptation in 2013. More than nine out of ten municipalities state that they have been affected by climate change and/or extreme weather events in this survey. Overall, climate adaptation work among the municipalities has continued to increase in each of our surveys, but the rate of increase has slowed down since the last survey in 2021. Statistical analyzes show that the strongest driving force in our data to implement climate adaptation measures is still being affected by natural damage. That the impact of natural damage temporarily decreased after 2019 for most municipalities, after having increased before that, can explain that the rate of increase in climate adaptation work has decreased since the 2021 survey. As a result of the decreasing rate of the increase in climate adaptation work in recent years, parts of the recommendations in the survey from 2021 are sharpened and repeated. The municipalities need to switch from a reactive to a more proactive climate adaptation work and the county administrations ensure their tasks to coordinate and follow up the municipalities' climate adaptation work. Without preventive climate adaptation work, the municipalities are forced to act reactively to the effects of climate change. The risk is then that the municipalities do not plan and prioritize resources and proactive measures in a timely and efficient manner. In the 2023 ranking of the municipalities' climate adaptation work, Norrköping and Boden got a total of 33 points each, where Norrköping received a higher total score in the decisive questions. The average score was 16.5 points among the 194 municipalities that responded.
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| 48. |
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| 49. |
- Huuhtanen, Jani, et al.
(author)
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Single-cell analysis of immune recognition in chronic myeloid leukemia patients following tyrosine kinase inhibitor discontinuation
- 2024
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In: Leukemia. - : Springer Nature. - 0887-6924 .- 1476-5551. ; 38, s. 109-125
-
Journal article (peer-reviewed)abstract
- Immunological control of residual leukemia cells is thought to occur in patients with chronic myeloid leukemia (CML) that maintain treatment-free remission (TFR) following tyrosine kinase inhibitor (TKI) discontinuation. To study this, we analyzed 55 single-cell RNA and T cell receptor (TCR) sequenced samples (scRNA+TCRαβ-seq) from patients with CML (n = 13, N = 25), other cancers (n = 28), and healthy (n = 7). The high number and active phenotype of natural killer (NK) cells in CML separated them from healthy and other cancers. Most NK cells in CML belonged to the active CD56dim cluster with high expression of GZMA/B, PRF1, CCL3/4, and IFNG, with interactions with leukemic cells via inhibitory LGALS9-TIM3 and PVR-TIGIT interactions. Accordingly, upregulation of LGALS9 was observed in CML target cells and TIM3 in NK cells when co-cultured together. Additionally, we created a classifier to identify TCRs targeting leukemia-associated antigen PR1 and quantified anti-PR1 T cells in 90 CML and 786 healthy TCRβ-sequenced samples. Anti-PR1 T cells were more prevalent in CML, enriched in bone marrow samples, and enriched in the mature, cytotoxic CD8+TEMRA cluster, especially in a patient maintaining TFR. Our results highlight the role of NK cells and anti-PR1 T cells in anti-leukemic immune responses in CML.
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| 50. |
- Ikonen, Hanna-Mari, et al.
(author)
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Not just daycare : nordic mothers in research, development and innovation navigating work and childcare
- 2024
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In: Community, Work and Family. - : Routledge. - 1366-8803 .- 1469-3615. ; 27:2, s. 208-224
-
Journal article (peer-reviewed)abstract
- Nordic welfare policies mitigate work-childcare reconciliation; however, they are not enough for mothers working in intensive work cultures. In addition, there are differences among the three Nordic states in both work-family policies and cultural norms as to how they should be used. In this article, we study the resources mothers who work in research, development and innovation (R&D&I) in Finland, Norway and Sweden rely on in their work-childcare reconciliation. Thematic analysis of interviews with 74 professionals resulted in identifying four main resources: father involvement, parental leave system and daycare, flexible working, and grandparent help and networks. Our analysis brings to view the blind spots in work and childcare reconciliation that Nordic care policies and flexible work schemes do not cover in the case of professional R&D&I mothers. We find that the role of fathers is overarching, as it regulates which of the other resources are used and how. We also argue that the role grandparents play as a resource is understudied.
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