| 1. |
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| 2. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Abelev, Betty, et al.
(author)
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Measurement of prompt J/psi and beauty hadron production cross sections at mid-rapidity in pp collisions at root s=7 TeV
- 2012
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In: Journal of High Energy Physics. - 1029-8479. ; :11
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Journal article (peer-reviewed)abstract
- The ALICE experiment at the LHC has studied J/psi production at mid-rapidity in pp collisions at root s = 7 TeV through its electron pair decay on a data sample corresponding to an integrated luminosity L-int = 5.6 nb(-1). The fraction of J/psi from the decay of long-lived beauty hadrons was determined for J/psi candidates with transverse momentum p(t) > 1,3 GeV/c and rapidity vertical bar y vertical bar < 0.9. The cross section for prompt J/psi mesons, i.e. directly produced J/psi and prompt decays of heavier charmonium states such as the psi(2S) and chi(c) resonances, is sigma(prompt J/psi) (p(t) > 1.3 GeV/c, vertical bar y vertical bar < 0.9) = 8.3 +/- 0.8(stat.) +/- 1.1 (syst.)(-1.4)(+1.5) (syst. pol.) mu b. The cross section for the production of b-hadrons decaying to J/psi with p(t) > 1.3 GeV/c and vertical bar y vertical bar < 0.9 is a sigma(J/psi <- hB) (p(t) > 1.3 GeV/c, vertical bar y vertical bar < 0.9) = 1.46 +/- 0.38 (stat.)(-0.32)(+0.26) (syst.) mu b. The results are compared to QCD model predictions. The shape of the p(t) and y distributions of b-quarks predicted by perturbative QCD model calculations are used to extrapolate the measured cross section to derive the b (b) over bar pair total cross section and d sigma/dy at mid-rapidity.
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| 4. |
- Abelev, Betty, et al.
(author)
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Underlying Event measurements in pp collisions at root s=0.9 and 7 TeV with the ALICE experiment at the LHC
- 2012
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In: Journal of High Energy Physics. - 1029-8479. ; :7
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Journal article (peer-reviewed)abstract
- We present measurements of Underlying Event observables in pp collisions at root s = 0 : 9 and 7 TeV. The analysis is performed as a function of the highest charged-particle transverse momentum p(T),L-T in the event. Different regions are defined with respect to the azimuthal direction of the leading (highest transverse momentum) track: Toward, Transverse and Away. The Toward and Away regions collect the fragmentation products of the hardest partonic interaction. The Transverse region is expected to be most sensitive to the Underlying Event activity. The study is performed with charged particles above three different p(T) thresholds: 0.15, 0.5 and 1.0 GeV/c. In the Transverse region we observe an increase in the multiplicity of a factor 2-3 between the lower and higher collision energies, depending on the track p(T) threshold considered. Data are compared to PYTHIA 6.4, PYTHIA 8.1 and PHOJET. On average, all models considered underestimate the multiplicity and summed p(T) in the Transverse region by about 10-30%.
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| 5. |
- Bentham, James, et al.
(author)
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A century of trends in adult human height
- 2016
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In: eLIFE. - 2050-084X. ; 5
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Journal article (peer-reviewed)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.522.7) and 16.5 cm (13.319.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 6. |
- Bentham, James, et al.
(author)
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A century of trends in adult human height
- 2016
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In: eLIFE. - : eLife Sciences Publications Ltd. - 2050-084X. ; 5
-
Journal article (peer-reviewed)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.5–22.7) and 16.5 cm (13.3– 19.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8– 144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 7. |
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| 8. |
- Abelev, Betty, et al.
(author)
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Long-range angular correlations on the near and away side in p-Pb collisions at root S-NN=5.02 TeV
- 2013
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In: Physics Letters. Section B: Nuclear, Elementary Particle and High-Energy Physics. - : Elsevier BV. - 0370-2693. ; 719:1-3, s. 29-41
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Journal article (peer-reviewed)abstract
- Angular correlations between charged trigger and associated particles are measured by the ALICE detector in p-Pb collisions at a nucleon-nucleon centre-of-mass energy of 5.02 TeV for transverse momentum ranges within 0.5 < P-T,P-assoc < P-T,P-trig < 4 GeV/c. The correlations are measured over two units of pseudorapidity and full azimuthal angle in different intervals of event multiplicity, and expressed as associated yield per trigger particle. Two long-range ridge-like structures, one on the near side and one on the away side, are observed when the per-trigger yield obtained in low-multiplicity events is subtracted from the one in high-multiplicity events. The excess on the near-side is qualitatively similar to that recently reported by the CMS Collaboration, while the excess on the away-side is reported for the first time. The two-ridge structure projected onto azimuthal angle is quantified with the second and third Fourier coefficients as well as by near-side and away-side yields and widths. The yields on the near side and on the away side are equal within the uncertainties for all studied event multiplicity and p(T) bins, and the widths show no significant evolution with event multiplicity or p(T). These findings suggest that the near-side ridge is accompanied by an essentially identical away-side ridge. (c) 2013 CERN. Published by Elsevier B.V. All rights reserved.
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| 9. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 10. |
- Kehoe, Laura, et al.
(author)
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Make EU trade with Brazil sustainable
- 2019
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Journal article (other academic/artistic)
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| 11. |
- Turkenburg, Wim C, et al.
(author)
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Renewable Energy Technologies
- 2000
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In: World energy assessment: energy and the challenge of sustainability. - 9211261260 ; , s. 219-272
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Book chapter (peer-reviewed)abstract
- In 1998 renewable energy sources supplied 56 ± 10 exajoules,or about 14 percent of world primary energy consumption. Thesupply was dominated by traditional biomass (38 ± 10 exajoules a year. Other major contributions came from large hydropower (9 exajoules a year) and from modern biomass (7 exajoules). The contribution of all other renewables — small hydropower, geothermal, wind, solar, and marineenergy — was about 2 exajoules. That means that the energy supply from new renewables was about 9 exajoules (about 2 percent of world consumption). The commercial primary energy supply from renewable sources was 27 ± 6 exajoules (nearly 7 percent of world consumption), with 16 ± 6 exajoules from biomass. Renewable energy sources can meet many times the present world energy demand, so their potential is enormous. They can enhance diversity in energy supply markets, secure long-term sustainable energy supplies, and reduce local and global atmospheric emissions. They can also provide commercially attractive options to meet specific needs for energy services (particularly in developing countries and rural areas), create new employment opportunities, and offer possibilities for local manufacturing of equipment. There are many renewable technologies. Although often commerciallyavailable, most are still at an early stage of development and not technically mature. They demand continuing research, development, and demonstration efforts. In addition, few renewable energy technologies can compete with conventional fuels on cost, except in some niche markets. But substantial cost reductions can be achieved for most renewables, closing gaps and making them more competitive. That will require further technology development and market deployment — and boosting production capacities to mass production. For the long term and under very favourable conditions, the lowest cost to produce electricity might be $0.01–0.02 a kilowatt-hour for geothermal, $0.03 a kilowatt-hour for wind and hydro, $0.04 a kilowatt-hour for solar thermal and biomass, and $0.05–0.06 a kilowatt-hour for photovoltaics and marine currents. The lowest cost to produce heat might be $0.005 a kilowatt-hour for geothermal, $0.01 a kilowatt-hour for biomass, and $0.02–0.03 a kilowatt-hour for solar thermal. The lowest cost to produce fuels might be $1.5 a gigajoule for biomass, $6–7 a gigajoule for ethanol, $7–10 a gigajoule for methanol, and $6–8 a gigajoule for hydrogen. Scenarios investigating the potential of renewables reveal that they might contribute 20–50 percent of energy supplies in the second half of the 21st century. A transition to renewables-based energy systems would have to rely on: Successful development and diffusion of renewable energy technologies that become more competitive through cost reductions from technological and organisational developments. Political will to internalise environmental costs and other externalities that permanently increase fossil fuel prices. Many countries have found ways to promote renewables. As renewable energy activities grow and require more funding, the tendency in many countries is to move away from methods that let taxpayers carry the burden of promoting renewables, towards economic and regulatory methods that let energy consumers carry the burden.
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| 12. |
- Arvizu, Dan, et al.
(author)
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Summary for Policy Makers: Intergovernmental Panel on Climate Change Special Report Renewable Energy Sources (SRREN)
- 2011
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Reports (other academic/artistic)abstract
- The Working Group III Special Report on Renewable Energy Sources and Climate Change Mitigation (SRREN) presents an assessment of the literature on the scientific, technological, environmental, economic and social aspects of the contribution of six renewable energy (RE) sources to the mitigation of climate change. It is intended to provide policy relevant information to governments, intergovernmental processes and other interested parties. This Summary for Policymakers provides an overview of the SRREN, summarizing the essential findings. The SRREN consists of 11 chapters. Chapter 1 sets the context for RE and climate change; Chapters 2 through 7 provide information on six RE technologies, and Chapters 8 through 11 address integrative issues.
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| 13. |
- Arvizu, Dan, et al.
(author)
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Technical Summary
- 2011
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In: IPCC Special Report on Renewable Energy Sources and Climate Change Mitigation.
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Book chapter (peer-reviewed)
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| 14. |
- Harahap, Fumi, 1983-
(author)
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Exploring synergies between the palm oil industry and bioenergy production in Indonesia
- 2020
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Doctoral thesis (other academic/artistic)abstract
- Climate change along with increasing demand for food and fuel call for sustainable use of natural resources. One way to address these concerns is through efficient use of resources, which is also vital for the achievement of the Sustainable Development Goals and the Paris Agreement. In this context, the sustainable and efficient use of resources in the palm oil industry is an interesting case to scrutinise. This is particularly important for Indonesia, the leading palm oil producer in the world. Large quantities of oils and biomass are generated from oil palm plantations and processing, presenting the potential for the development of bio-based production systems. However, at present, sustainability is a matter of great concern in this industry, including land use issues and the fact that large portions of the residues generated are untreated, releasing greenhouse gas emissions, and imposing environmental threats.This doctoral thesis aims at exploring how resource efficiency can be enhanced in the palm oil industry. Three research questions are posed to address the objective. The first question examines the sectoral policy goals of biofuel, agriculture, climate, and forestry and their requirements for land. The second question is focused on new industrial configurations for efficient use of palm oil biomass for bioenergy production. The final question summarises the role of enhancing resource efficiency in the palm oil industry with regards to meeting the national bioenergy targets, which include 5.5 GWe installed capacity and biofuel blending with fossil fuels (30% biodiesel blending with diesel and 20% ethanol blending with gasoline) in the transport, industry, and power sectors. The research questions are explored using three main methods: policy coherence analysis, techno-economic analysis, and a spatio-temporal optimisation model (BeWhere Indonesia).The thesis identifies areas in which policy formulation, in terms of sectoral land allocation, can be improved. Adjustments and improvements in policy formulation and implementation are crucial for land allocation. The inconsistencies in the use of recognised land classifications in the policy documents, the unclear definition of specific land categories, and the multiple allocation of areas, should be addressed immediately to ensure coherent sectoral policies on land allocation. This can lead to more effective policy implementation, reduce pressure on land, enhance synergies, and resolve conflicts between policy goals.The transition towards a more sustainable palm oil industry requires a shift from current traditional practices. Such transition involves efficient use of palm oil biomass resources through improved biomass conversion technologies and integration of palm oil mills with energy production in biorefinery systems. The upgrading of the conventional production systems can serve multiple purposes including clean energy access and production of clean fuels for the transport, industry, and power sectors, ultimately helping the country meet its renewable energy and sustainable development targets, along with reduced emissions. More specifically, the efficient use of biomass and co-production of bioenergy carriers in biorefineries can enable Indonesia to reach its targets for bioenergy installed capacity and bio-based blending.At present, many government policies in Indonesia are working in the right direction. Nevertheless, various barriers still need to be overcome so that resource efficiency can be improved. This includes harnessing the full potential of bioenergy in the palm oil industry. There is room for enhancing the sustainability of the palm oil industry in Indonesia with adjustments to existing policies and practices, as shown in this thesis. First, guidance across sectoral policies can help to coordinate the use of basic resources. Second, the shift from traditional practices requires a strategy that includes improvement in agricultural practices (i.e., higher yields), infrastructure for biomass conversion technologies together with improved grid connectivity, and adoption of a biorefinery system. Strengthening policy support is needed to promote such a comprehensive shift. Third, various programmes can forge partnerships between oil palm plantations, the palm oil mills, and energy producers to ensure the development of sustainable industrial practices. A sustainable palm oil industry will improve resource and cost efficiency, and help open international markets for Indonesian products. This could pave the way for an enhanced role for the Indonesian palm oil industry in global sustainability efforts.
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| 15. |
- Hirata, Rafael Y. S., et al.
(author)
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Platinum nanoparticle-based microreactors protect against the behavioral and neurobiological consequences of chronic stress exposure
- 2022
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In: Brain Research Bulletin. - : PERGAMON-ELSEVIER SCIENCE LTD. - 0361-9230 .- 1873-2747. ; 190
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Journal article (peer-reviewed)abstract
- Excitotoxicity is described as the exacerbated activation of glutamate AMPA and NMDA receptors that leads to neuronal damage, and ultimately to cell death. Astrocytes are responsible for the clearance of 80-90% of syn-aptically released glutamate, preventing excitotoxicity. Chronic stress renders neurons vulnerable to excitotox-icity and has been associated to neuropsychiatric disorders, i.e., anxiety. Microreactors containing platinum nanoparticles (Pt-NP) and glutamate dehydrogenase have shown in vitro activity against excitotoxicity. The purpose of the present study was to investigate the in vivo effects of these microreactors on the behavioral and neurobiological effects of chronic stress exposure. Rats were either unstressed or exposed for 2 weeks to an unpredictable chronic mild stress paradigm (UCMS), administered intra-ventral hippocampus with the micro -reactors (with or without the blockage of astrocyte functioning), and seven days later tested in the elevated T -maze (ETM; Experiment 1). The ETM allows the measurement of two defensive responses, avoidance and escape, in terms of psychopathology respectively related to generalized anxiety and panic disorder. Locomotor activity in an open field was also measured. Since previous evidence shows that stress inhibits adult neurogenesis, we evaluated the effects of the different treatments on the number of cells expressing the marker of migrating neuroblasts doublecortin (DCX) in the dorsal and ventral hippocampus (Experiment 2). Results showed that UCMS induces anxiogenic effects, increases locomotion, and decreases the number of DCX cells in the dorsal and ventral hippocampus, effects that were counteracted by microreactor administration. This is the first study to demonstrate the in vivo efficacy of Pt-NP against the behavioral and neurobiological effects of chronic stress exposure.
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| 16. |
- Linderholm, Carl Johan, 1976, et al.
(author)
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1st International Conference on Negative CO2 Emissions - Summary and Highlights
- 2018
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In: GHGT 2018 - 14th International Conference on Greenhouse Gas Control Technologies. - : International Energy Agency Greenhouse Gas, IEAGHG.
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Conference paper (peer-reviewed)abstract
- Negative CO2 emissions technologies include a number of technologies and biospheric storage options, the objective of which is the removal of atmospheric CO2 and thus the limitation of future global warming. An international conference on negative emissions technologies was conceived to meet the need for a broader understanding of the possibilities and challenges facing these technologies. The International Conference on Negative CO2 Emissions was held in May 22-24, 2018, at Chalmers University of Technology, Gothenburg, Sweden. The conference was organized by Chalmers with support from the Global Carbon Project, the City of Gothenburg, Nordic Energy Research, ECOERA, the Center for Carbon Removal, Göteborg Energi, Stockholm Exergi, and the International Energy Agency, i.e. IEAGHG, IEAIETS and IEA Bioenergy. The purpose of the conference was to bring together a wide range of scientific and technological disciplines and stakeholders, in order to engage in various aspects of research relating to negative CO2 emissions. This included various negative emission technologies, socio-economic and climate modelling, and climate policies and incentives. The conference was a major scientific event and the first in a conference series. The next conference will be held in the spring of 2020. This paper reports highlights and important messages from the conference.
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| 17. |
- Scutelnic, Adrian, et al.
(author)
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Characteristics and outcomes of cerebral venous thrombosis associated with COVID-19
- 2024
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In: EUROPEAN STROKE JOURNAL. - 2396-9873 .- 2396-9881.
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Journal article (peer-reviewed)abstract
- Introduction: Previous reports and meta-analyses derived from small case series reported a mortality rate of up to 40% in patients with coronavirus disease 2019 associated cerebral venous thrombosis (COVID-CVT). We assessed the clinical characteristics and outcomes in an international cohort of patients with COVID-CVT. Patients and methods: This was a registry study of consecutive COVID-CVT patients diagnosed between March 2020 and March 2023. Data collected by the International Cerebral Venous Thrombosis Consortium from patients with CVT diagnosed between 2017 and 2018 served as a comparison. Outcome analyses were adjusted for age and sex. Results: We included 70 patients with COVID-CVT from 23 hospitals in 15 countries and 206 controls from 14 hospitals in 13 countries. The proportion of women was smaller in the COVID-CVT group (50% vs 68%, p < 0.01). A higher proportion of COVID-CVT patients presented with altered mental state (44% vs 25%, p < 0.01), the median thrombus load was higher in COVID-CVT patients (3 [IQR 2-4] vs 2 [1-3], p < 0.01) and the length of hospital stay was longer compared to controls (11 days [IQR 7-20] vs 8 [4-15], p = 0.02). In-hospital mortality did not differ (5/67 [7%, 95% CI 3-16] vs 7/206 [3%, 2-7], aOR 2.6 [95% CI 0.7-9]), nor did the frequency of functional independence after 6 months (modified Rankin Scale 0-2; 45/58 [78%, 95% CI 65-86] vs 161/185 [87%, 81-91], aOR 0.5 [95% CI 0.2-1.02]). Conclusion: In contrast to previous studies, the in-hospital mortality rate and functional outcomes during follow-up did not differ between COVID-CVT patients and the pre-COVID-19 controls.
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