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- Dunham, I, et al.
(author)
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The DNA sequence of human chromosome 22
- 1999
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 402:6761, s. 489-495
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Journal article (peer-reviewed)
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2. |
- Nguyen, N, et al.
(author)
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Chromosomal sequencing using a PCR-based biotin-capture method allowed isolation of the complete gene for the outer membrane protein A of Klebsiella pneumoniae
- 1998
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In: Gene. - 0378-1119 .- 1879-0038. ; 210:1, s. 93-101
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Journal article (peer-reviewed)abstract
- By employing a novel biotin-and PCR-assisted capture method, which allows determination of unknown sequences on chromosomal DNA. the gene for the outer membrane protein A (OmpA) of Klebsiella pneumoniae has been isolated and sequenced to completion. The method involves linear amplification of DNA from a biotinylated primer annealing to a region with known sequence. After capture of the amplified single-stranded DNA on to paramagnetic beads, unspecifically annealing primers, i.e. arbitrary primers, were used to generate fragments with only partly determined nt sequences. The homology of the sequenced gene to ompAs of related bacteria is discussed. The ompA gene was assembled for intracellular expression in Escherichia coli, and two different fusion proteins were produced and recovered with good yields. The importance of the novel chromosomal sequencing method for gene isolation in general and the potential use of the OmpA fusion proteins are discussed. (C) 1998 Elsevier Science B.V.
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3. |
- Nguyen, Son Tien, 1953-, et al.
(author)
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Photoluminescence and Zeeman effect in chromium-doped 4H and 6H SiC
- 1999
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In: Journal of Applied Physics. - : AIP Publishing. - 0021-8979 .- 1089-7550. ; 86:8, s. 4348-
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Journal article (peer-reviewed)abstract
- Photoluminescence (PL) and Zeeman effect measurements in near-infrared luminescence bands in Cr-doped 4H and 6H SiC are presented. The PL spectrum consists of two no-phonon lines (NPLs) at 1.1583 and 1.1898 eV in 4H SiC and three NPLs at 1.1556, 1.1797, and 1.1886 eV in 6H SiC. The observed Zeeman splittings and temperature dependence studies reveal the spin triplet of the ground state and the orbital doublet structure of the excited state of the Cr-related center. All the triplets have almost isotropic g values close to 2 with trigonal symmetry and small zero-field splitting values D. In contrast, the effective g values of the excited state of the center are very anisotropic with g|| in the range of 0.22-0.64 and g[perpendicular] = 0 for different NPLs in both polytypes. Based on the Zeeman results, the PL is attributed to the internal transition 1E(D)-->3A2(F) within the d shell of a substitutional, neutral chromium (Cr4 + ) in the 3d2 electronic configuration.
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