| 1. |
- Hemström, Petrus, et al.
(author)
-
Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins.
- 2006
-
In: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 29:1, s. 25-32
-
Journal article (peer-reviewed)abstract
- Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.
|
|
| 2. |
- Nordborg, Anna, et al.
(author)
-
Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths
- 2005
-
In: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 28:17, s. 2401-2406
-
Journal article (peer-reviewed)abstract
- The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 mu m ID capillaries and their application for mu-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 mu m suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.
|
|
