1. |
- Rich, Rebecca L., et al.
(author)
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A global benchmark study using affinity-based biosensors
- 2009
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In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
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Journal article (peer-reviewed)abstract
- To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
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2. |
- Thompson, Rodger I., et al.
(author)
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A molecular probe of dark energy
- 2008
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In: Advances in Space Research. - : Elsevier BV. - 1879-1948 .- 0273-1177. ; 42:3, s. 596-598
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Journal article (peer-reviewed)abstract
- Many theoretical models of dark energy invoke rolling scaler fields which in turn predict time varying values of the fundamental constants. Establishing the value of the fundamental constants at various times in the universe can probe and test the various dark energy theories. One of the constants that is predicted to vary is the ratio of the electron to proton mass μ. It was established early on that molecular spectra are sensitive to the value of μ and can be used as probes of that value. This article describes the use of the spectrum of molecular hydrogen in high redshift Damped Lyman Alpha systems (DLAs) as a sensitive probe of the time evolution of μ.
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3. |
- Thompson, Rodger I., et al.
(author)
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Alternative data reduction procedures for UVES: Wavelength calibration and spectrum addition
- 2009
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In: New Astronomy. - : Elsevier BV. - 1384-1076 .- 1384-1092. ; 14:4, s. 379-390
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Journal article (peer-reviewed)abstract
- This paper addresses alternative procedures to the ESO supplied pipeline procedures for the reduction of UVES spectra of two quasar spectra to determine the value of the fundamental constant μ=Mp/Me at early times in the universe. The procedures utilize intermediate product images and spectra produced by the pipeline with alternative wavelength calibration and spectrum addition methods. Spectroscopic studies that require extreme wavelength precision need customized wavelength calibration procedures beyond that usually supplied by the standard data reduction pipelines. An example of such studies is the measurement of the values of the fundamental constants at early times in the universe. This article describes a wavelength calibration procedure for the UV–visual Echelle spectrometer on the very large telescope, however, it can be extended to other spectrometers as well. The procedure described here provides relative wavelength precision of better than 3×10-7 for the long-slit Thorium–Argon calibration lamp exposures. The gain in precision over the pipeline wavelength calibration is almost entirely due to a more exclusive selection of Th/Ar calibration lines.
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4. |
- Thompson, Rodger I., et al.
(author)
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An observational determination of the proton to electron mass ratio in the early Universe
- 2009
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In: Astrophysical Journal. - 1538-4357 .- 0004-637X. ; 703:2009 October 1, s. 1648 - 1662
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Journal article (peer-reviewed)abstract
- In an effort to resolve the discrepancy between two measurements of the fundamental constant μ, the proton to electron mass ratio, at early times in the universe we reanalyze the same data used in the earlier studies. Our analysis of the molecular hydrogen absorption lines in archival Very Large Telescope/Ultraviolet and Visible Echelle Spectrometer (UVES) spectra of the damped Lyman alpha systems in the quasi-stellar objects Q0347-383 and Q0405-443 yields a combined measurement of a Δμ/μ value of (–7 ± 8) × 10–6, consistent with no change in the value of μ over a time span of 11.5 Gyr. Here, we define Δμ as (μ z – μ0) where μ z is the value of μ at a redshift of z and μ0 is the present-day value. Our null result is consistent with the recent measurements of King et al., Δμ/μ = (2.6 ± 3.0) × 10–6, and inconsistent with the positive detection of a change in μ by Reinhold et al. Both of the previous studies and this study are based on the same data but with differing analysis methods. Improvements in the wavelength calibration over the UVES pipeline calibration is a key element in both of the null results. This leads to the conclusion that the fundamental constant μ is unchanged to an accuracy of 10–5 over the last 80% of the age of the universe, well into the matter dominated epoch. This limit provides constraints on models of dark energy that invoke rolling scalar fields and also limits the parameter space of supersymmetric or string theory models of physics. New instruments, both planned and under construction, will provide opportunities to greatly improve the accuracy of these measurements.
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