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- Agius, Stephanie C, et al.
(author)
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The internal rotenone-insensitive NADPH dehydrogenase contributes to malate oxidation by potato tuber and pea leaf mitochondria
- 1998
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In: Physiologia Plantarum. - : Wiley. - 0031-9317. ; 104:3, s. 329-336
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Journal article (peer-reviewed)abstract
- Inside-out submitochondrial particles from both potato (Solanum tuberosum L. cv. Bintje) tubers and pea (Pisum sativum L. cv. Oregon) leaves possess three distinct dehydrogenase activities: Complex I catalyzes the rotenone-sensitive oxidation of deamino-NADH, NDin(NADPH) catalyzes the rotenone-insensitive and Ca2+-dependent oxidation of NADPH and NDin(NADH) catalyzes the rotenone-insensitive and Ca2+-independent oxidation of NADH. Diphenylene iodonium (DPI) inhibits complex I, NDin(NADPH) and NDin (NADH) activity with a Ki of 3.7, 0.17 and 63 µM, respectively, and the 400-fold difference in Ki between the two NDin made possible the use of DPI inhibition to estimate NDin (NADPH) contribution to malate oxidation by intact mitochondria. The oxidation of malate in the presence of rotenone by intact mitochondria from both species was inhibited by 5 µM DPI. The maximum decrease in rate was 10–20 nmol O2 mg-1 min-1. The reduction level of NAD(P) was manipulated by measuring malate oxidation in state 3 at pH 7.2 and 6.8 and in the presence and absence of an oxaloacetate-removing system. The inhibition by DPI was largest under conditions of high NAD(P) reduction. Control experiments showed that 125 µM DPI had no effect on the activities of malate dehydrogenase (with NADH or NADPH) or malic enzyme (with NAD+ or NADP+) in a matrix extract from either species. Malate dehydrogenase was unable to use NADP+ in the forward reaction. DPI at 125 µM did not have any effect on succinate oxidation by intact mitochondria of either species. We conclude that the inhibition caused by DPI in the presence of rotenone in plant mitochondria oxidizing malate is due to inhibition of NDin(NADPH) oxidizing NADPH. Thus, NADP turnover contributes to malate oxidation by plant mitochondria.
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| 3. |
- Bountouvi, Evangelia, et al.
(author)
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Longitudinal Data in Patients with Niemann-Pick Type C Disease Under Combined High Intrathecal and Low Intravenous Dose of 2-hydroxylpropyl-β-cyclodextrin.
- 2021
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In: Innovations in clinical neuroscience. - 2158-8333. ; 18:1-3, s. 11-16
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Journal article (peer-reviewed)abstract
- Niemann-Pick Type C disease (NPC) is a rare, incurable, autosomal-recessive, lysosomal storage disorder with protean and progressive neurovisceral manifestations characterized by accumulation of intracellular unesterified cholesterol. The investigational use of 2-hydroxypropyl-beta-cyclodextrin (HP-β-CD) in the treatment of NPC has shown promising results in improving life expectancy and reducing neurological damage in this patient population. This case report describes two children with the neurological form of NPC: a 5-year-old male patient in advanced stage of the disease and an 11-year-old female patient in moderately advanced stage. Despite treatment with the enzyme inhibitor, miglustat, both patients continued to exhibit severe neurodegeneration. High intrathecal (900mg) and low intravenous (350-500mg/kg) doses of HP-β-CD (Trappsol®Cyclo™) were administrated twice monthly to the patients in addition to miglustat therapy. The patients were monitored clinically as well as by imaging, laboratory, and biomarker (e.g., total tau protein [T-tau]; phosphorylated tau [P-tau]; neurofilament light [NFL], oxysterols) studies over a period of 16 to 22 months. The combination therapy of miglustat and HP-β-CD resulted in disease stabilization in both patients. The combination therapy demonstrated a good safety profile, and no adverse effects on hearing were observed. Additionally, CSF biomarkers appeared useful in monitoring neuronal damage. Large, randomized studies are needed to confirm these findings.
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| 6. |
- Chen, Zihan, et al.
(author)
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Design of Compact Passive Tag Antenna for Practical RFID Applications
- 2014
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In: PIERS 2014 GUANGZHOU. - : ELECTROMAGNETICS ACAD. - 9781934142288 ; , s. 680-685
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Conference paper (peer-reviewed)abstract
- The communication quality between an RFID tag and a reader in a RFID network, especially the parameters of the passive tag antenna such as reading range, directivity and return loss, is affected by several factors from the environment. For practical RFID application, since the environment is complex and volatile, these factors include metallic surface of products, absorptions and impacts from neighbor tag antennas like scattering and mutual coupling. In this paper, it consists of the analysis of impacts caused by these factors and the design of passive tag antennas with resonant frequency 915 MHz able to be used in practical RFID applications against those impacts. Comparing with former ones, the proposed antenna reduces the size of the tag antenna to approximately commercial standard (18 mm x 48 mm x 3 mm) by using certain structures applied in the unit cell of metamaterial or HIS, while the properties including directivity, return loss and input impedance are still appropriate for RFID applications. Simulation results from HFSS indicates that at 915 MHz, the return loss can be about 21 dB, as most of the power can be radiated into the space towards the receiver. Meanwhile, the input impedance Z(0) = 9.4738 + j143.9189 Omega, perfectly matching the impedance of the RFID chip placed on the top surface of the antenna. The above improved parameters play an important role in prolonging the reading range.
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| 8. |
- Huvila, Isto, 1976-
(author)
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Towards information leadership
- 2014
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In: Aslib Journal of Info Mgmt. - 2050-3806. ; 66:6, s. 663-677
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Journal article (peer-reviewed)abstract
- Purpose The purpose of this paper is to propose information leadership as a concept for describing the activity of leading information processes in organisations and in the society, and consequently, to distinguish and articulate the influence and consequences of making decisions about the use, organisation and management of information resources and information infrastructures. Design/methodology/approach To complement the earlier observations of the usefulness of making an analytical distinction between information and knowledge, and management and leadership within information management/knowledge management, this paper posits that it is useful to distinguish information and knowledge leadership as two separate yet inherently connected processes of orchestrating respectively information infrastructures and resources, and the processes of knowing in an organisation. Findings This paper defines information leadership as the leadership of information resources and infrastructures in contrast to the leadership of social knowledge processes, knowing and organisational learning. The distinction helps to explicate the informational premises of knowledge in organisations, articulate the difference of the infrastructural and social forms of leadership and to be more explicit about the respective consequences of information and information infrastructures and knowledge held and practised within an organisation and on its boundaries. Practical implications The concept of information leadership can be used both for describing the activity of leading the development and using information resources and infrastructures in organisations, and for articulating the influence and consequences of making decisions about the use, organisation and management of information resources and information infrastructures. Originality/value To the knowledge of the authors, this is the first time information leadership is defined comprehensively in the context of information and knowledge management and specifically in relation to knowledge leadership.
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