| 1. |
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| 2. |
- Andersson, Martin, et al.
(author)
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- 2009
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Conference paper (other academic/artistic)
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| 3. |
- Aad, G., et al.
(author)
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- 2013
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swepub:Mat__t (peer-reviewed)
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| 4. |
- Kanje, Sara, 1986-
(author)
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Engineering of small IgG binding domains for antibody labelling and purification
- 2016
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Doctoral thesis (other academic/artistic)abstract
- In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. Antibodies used for these purposes are often ”man-made”, by immunising animals with the desired target, or by selections from combinatorial libraries. Naturally, antibodies are part of the immune defence protecting us from foreign attacks from e.g. bacteria or viruses. Some bacteria have evolved surface proteins that can bind to proteins abundant in the blood, like antibodies and serum albumin. By doing so, the bacteria can cover themselves in the host’s own proteins and through that evade being detected by the immune system. Two such proteins are Protein A from Staphylococcus aureus and Protein G from group C and G Streptococci. Both these proteins contain domains that bind to antibodies, one of which is denoted C2 (from Protein G) and another B (from Protein A). The B domain have been further engineered to the Z domain.In this thesis protein engineering has been used to develop variants of the C2 and Z domains for site-specific labelling of antibodies and for antibody purification with mild elution. By taking advantage of the domains’ inherent affinity for antibodies, engineering and design of certain amino acids or protein motifs of the domains have resulted in proteins with new properties. A photo crosslinking amino acid, p-benzoylphenylalanine, have been introduced at different positions to the C2 domain, rendering three new protein domains that can be used for site-specific labelling of antibodies at the Fc or Fab fragment. These domains were used for labelling antibodies with lanthanides and used for detection in a multiplex immunoassay. Moreover, a library of calcium-binding loops was grafted onto the Z domain and used for selection of a domain that binds antibodies in a calcium dependent manner. This engineered protein domain can be used for the purification of antibodies using milder elution conditions, by calcium removal, as compared to traditional antibody purification.
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| 5. |
- Mako, T., et al.
(author)
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Patients' perceptions of the meaning of good care in surgical care: a grounded theory study
- 2016
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In: Bmc Nursing. - : Springer Science and Business Media LLC. - 1472-6955. ; 15
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Journal article (peer-reviewed)abstract
- Background: Patients in surgical care have reported a fear of being discharged prior to sufficient recovery and a lack of control of their situation. Establishing the patient-nurse relationship is essential in the context of the care. The Swedish National Board of Health and Welfare has established indicators for good care for comparison, evaluation and improvement of the quality of the health care system. These indicators are knowledge-based, appropriate, safe, effective and equal health care, as well as care within a reasonable time and patient-centred care. Current core competence in nursing education include quality improvement, patient-centred care, teamwork and collaboration, using evidence-based practice, safety and informatics. This study investigates patients' perceptions of the meaning of good care in inpatient surgical care. Methods: Grounded theory according to Charmaz was chosen as the study design. Interviews were conducted with 13 patients from six surgical wards in the south of Sweden in 2014-2015. Results: The results showed that patients in surgical care perceived good care as being safe, as they were vulnerable and anxious. This could be achieved through accessible care, reliable care, caring attitudes and participating in one's own care. Patient participation was achieved by information and education and the possibility to affect their care. Conclusion: Patients need safety to experience good care. Caring attitudes and patient participation can be attained through patient-centred care. Bedside handover can improve patients' perceptions of accessible care and reliable care and can increase patient participation. Continuously maintaining competence and using evidence-based practice are needed to achieve reliable care.
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| 6. |
- Ye, Guozhu, et al.
(author)
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Recovery of Zn and Mn from spent alkaline batteries
- 2018
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In: Part of the The Minerals, Metals & Materials Series book series (MMMS). - Cham : Springer International Publishing. - 9783319721378 ; , s. 329-341
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Conference paper (peer-reviewed)abstract
- The most used technology for recycling of spent alkaline batteries consists of crushing, magnetic separation and processing of the nonmagnetic fraction, black mass, using a rotary kiln. In the process only zinc is recovered and all manganese in the black mass is lost. According to the EU regulation for recycling of batteries, more than 50% of batteries should be recovered. To meet the demand, Swerea MEFOS has together with a major battery recycler IsoLogistics in Finland and Sweden developed technologies for efficient recovery of zinc and manganese aiming for >50% recovery. This paper will focus on a treatment process using a DC furnace to produce a ZnO- and a MnO-product. The process has been demonstrated in pilot scale, 1, ton/h. The major outcomes will be highlighted. Another approach by using thermal vacuum technology for distillation of zinc metal has also been demonstrated in laboratory scale which is also reported in this paper.
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| 7. |
- Grage, M.M.-L., et al.
(author)
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Conformational disorder of a substituted polythiophene in solution revealed by excitation transfer
- 2001
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In: Chemical Physics Letters. - 0009-2614 .- 1873-4448. ; 339:1-2, s. 96-102
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Journal article (peer-reviewed)abstract
- Anisotropy and isotropic transient absorption decays of a polythiophene derivative in dilute solution have been measured and compared to calculated decays on Monte Carlo simulated polymer chains to obtain knowledge about the geometrical conformation of the polymer. The polymer chain conformations are generated by a partially correlated self-avoiding random walk. Excitation migration along the isolated chain is modeled assuming a hopping mechanism. This approach can be used to determine conformational disorder and possible realizations of the polymer chains in solution as well as the trapping time and migration length of the excitation. © 2001 Elsevier Science B.V.
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| 8. |
- Graham, Alastair G.C., et al.
(author)
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Rapid retreat of Thwaites Glacier in the pre-satellite era
- 2022
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In: Nature Geoscience. - : Springer Science and Business Media LLC. - 1752-0894 .- 1752-0908. ; 15, s. 706-713
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Journal article (peer-reviewed)abstract
- Understanding the recent history of Thwaites Glacier, and the processes controlling its ongoing retreat, is key to projecting Antarctic contributions to future sea-level rise. Of particular concern is how the glacier grounding zone might evolve over coming decades where it is stabilized by sea-floor bathymetric highs. Here we use geophysical data from an autonomous underwater vehicle deployed at the Thwaites Glacier ice front, to document the ocean-floor imprint of past retreat from a sea-bed promontory. We show patterns of back-stepping sedimentary ridges formed daily by a mechanism of tidal lifting and settling at the grounding line at a time when Thwaites Glacier was more advanced than it is today. Over a duration of 5.5 months, Thwaites grounding zone retreated at a rate of >2.1 km per year—twice the rate observed by satellite at the fastest retreating part of the grounding zone between 2011 and 2019. Our results suggest that sustained pulses of rapid retreat have occurred at Thwaites Glacier in the past two centuries. Similar rapid retreat pulses are likely to occur in the near future when the grounding zone migrates back off stabilizing high points on the sea floor.
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| 9. |
- Korakianitis, T, et al.
(author)
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Parametric performance of combined-cogeneration power plants with various power and efficiency enhancements
- 2005
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In: Journal of engineering for gas turbines and power. - : ASME International. - 0742-4795 .- 1528-8919. ; 127:1, s. 65-72
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Journal article (peer-reviewed)abstract
- The design-point performance characteristics of a wide variety of combined-cogeneration power plants, with different amounts of supplementary firing (or no supplementary firing), different amounts of steam injection (or no steam injection), different amounts of exhaust gas condensation, etc., without limiting these parameters to present-day limits are investigated. A representative power plant with appropriate components for these plant enhancements is developed. A computer program,is used to evaluate the performance of various power plants using standard inputs for component efficiencies, and the design-point performance of these plants is computed. The results are presented as thermal efficiency, specific power, effectiveness, and specific rate of energy in district heating. The performance of the simple-cycle gas turbine dominates the overall plant performance; the plant efficiency and power are mainly determined by turbine inlet temperature and compressor pressure ratio; increasing amounts of steam injection in the gas turbine increases the efficiency and power; increasing amounts of supplementary firing decreases the efficiency but increases the power; with sufficient amounts of supplementary team injection the exhaust-gas condensate is sufficient to make up for water lost in steam injection; and the steam-turbine power is a fraction (0.1 to 0.5) of the gas-turbine power output. Regions of "optimum" parameters for the power plant based oil design-point power hot-water demand, and efficiency are shown. A method,for fuel-cost allocation between electricity and hot water is recommended.
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| 10. |
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