Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations

• The quality of clinical biobank samples is crucial to their value for life sciences research. A number of factors related to the collection and storage of samples may affect the biomolecular composition. We have studied the effect of long-time freezer storage, chronological age at sampling, season and month of the year and on the abundance levels of 108 proteins in 380 plasma samples collected from 106 Swedish women. Storage time affected 18 proteins and explained 4.8-34.9% of the observed variance. Chronological age at sample collection after adjustment for storage-time affected 70 proteins and explained 1.1-33.5% of the variance. Seasonal variation had an effect on 15 proteins and month (number of sun hours) affected 36 proteins and explained up to 4.5% of the variance after adjustment for storage-time and age. The results show that freezer storage time and collection date (month and season) exerted similar effect sizes as age on the protein abundance levels. This implies that information on the sample handling history, in particular storage time, should be regarded as equally prominent covariates as age or gender and need to be included in epidemiological studies involving protein levels.

Subject headings

Medical and Health Sciences  (hsv)

Clinical Medicine  (hsv)

Family Medicine  (hsv)

Medicin och hälsovetenskap  (hsv)

Klinisk medicin  (hsv)

Allmän medicin  (hsv)

Keyword

Plasma proteins

Biobank

Covariate

Storage time

Sampling month

Proximity extension assay