Development of a molecular test to determine the vitality status of Norway spruce (Picea abies) seedlings during frozen storage

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Stattin, Eva,1958-Högskolan Dalarna,Skog- och träteknik,Skog och Träteknik (author)

Development of a molecular test to determine the vitality status of Norway spruce (Picea abies) seedlings during frozen storage

Article/chapterEnglish2012

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2012-04-04
Springer Science and Business Media LLC,2012
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:du-10366
https://urn.kb.se/resolve?urn=urn:nbn:se:du-10366URI
https://doi.org/10.1007/s11056-012-9320-1DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

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Open Access
In boreal forest regions, a great portion of forest tree seedlings are stored indoors in late autumn to prevent seedlings from outdoor winter damage. For seedlings to be able to survive in storage it is crucial that they store well and can cope with the dark and cold storage environment. The aim of this study was to search for genes that can determine the vitality status of Norway spruce (Picea abies (L.) Karst.) seedlings during frozen storage. Furthermore, the sensitivity of the ColdNSure (TM) test, a gene activity test that predicts storability was assessed. The storability of seedlings was tested biweekly by evaluating damage with the gene activity test and the electrolyte leakage test after freezing seedlings to -25 A degrees C (the SELdiff-25 method). In parallel, seedlings were frozen stored at -3 A degrees C. According to both methods, seedlings were considered storable from week 41. This also corresponded to the post storage results determined at the end of the storage period. In order to identify vitality indicators, Next Generation Sequencing (NGS) was performed on bud samples collected during storage. Comparing physiological post storage data to gene analysis data revealed numerous vitality related genes. To validate the results, a second trial was performed. In this trial, gene activity was better in predicting seedling storability than the conventional freezing test; this indicates a high sensitivity level of this molecular assay. For multiple indicators a clear switch between damaged and vital seedlings was observed. A collection of indicators will be used in the future development of a commercial vitality test.

Subject headings and genre

vitality test
forest tree seedlings
storability
Picea abies
next generation sequencing
gene expression
Energy and Built Environments
Energi och samhällsbyggnad

Added entries (persons, corporate bodies, meetings, titles ...)

Verhoef, Nathalie (author)
Balk, Peter (author)
van Wordragen, Monique (author)
Lindström, Anders,1949-Högskolan Dalarna,Skog- och träteknik,Skog och Träteknik(Swepub:du)ali (author)
Högskolan DalarnaSkog- och träteknik (creator_code:org_t)

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In:New forests: Springer Science and Business Media LLC43:5-6, s. 665-6780169-42861573-5095

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