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No evidence for xen...
No evidence for xenotropic murine leukemia-related virus infection in Sweden using internally controlled multiepitope suspension array serology
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- Blomberg, Jonas (författare)
- Uppsala universitet,Klinisk virologi
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- Blomberg, Fredrik (författare)
- Uppsala universitet,Klinisk virologi
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- Sjösten, Anna (författare)
- Uppsala universitet,Klinisk virologi
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visa fler...
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- Sheikholvaezin, Ali (författare)
- Uppsala universitet,Klinisk virologi
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Bolin-Wiener, Agnes (författare)
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- Elfaitouri, Amal (författare)
- Uppsala universitet,Klinisk virologi
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Hessel, Sanna (författare)
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Gottfries, Carl-Gerhard (författare)
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Zachrisson, Olof (författare)
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- Öhrmalm, Christina (författare)
- Uppsala universitet,Klinisk virologi
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- Jobs, Magnus (författare)
- Uppsala universitet,Högskolan Dalarna,Medicinsk vetenskap,Klinisk virologi
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Pipkorn, Ruediger (författare)
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(creator_code:org_t)
- American Society for Microbiology, 2012
- 2012
- Engelska.
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Ingår i: Clinical and Vaccine Immunology. - : American Society for Microbiology. - 1556-6811 .- 1556-679X. ; 19:9, s. 1399-1410
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Many syndromes have a large number of differential diagnoses, a situation which calls for multiplex diagnostic systems. Myalgic encephalomyelitis (ME), also named chronic fatigue syndrome (CFS), is a common disease of unknown etiology. A mouse retrovirus, xenotropic murine leukemia-related virus (XMRV), was found in ME/CFS patients and blood donors, but this was not corroborated. However, the paucity of serological investigations on XMRV in humans prompted us to develop a serological assay which cover many aspects of XMRV antigenicity. It is a novel suspension array method, using a multiplex IgG assay with nine recombinant proteins from the env and gag genes of XMRV and 38 peptides based on known epitopes of vertebrate gamma-retroviruses. IgG antibodies were sought in 520 blood donors and 85 ME/CFS patients and in positive-and negative-control sera from animals. We found no differences in seroreactivity between blood donors and ME/CFS patients for any of the antigens. This did not support an association between ME/CFS and XMRV infection. The multiplex serological system had several advantages: (i) biotinylated protein G allowed us to run both human and animal sera, which is essential because of a lack of XMRV-positive humans; (ii) a novel quality control was a pan-peptide positive-control rabbit serum; and (iii) synthetic XMRV Gag peptides with degenerate positions covering most of the variation of murine leukemia-like viruses did not give higher background than nondegenerate analogs. The principle may be used for creation of variant tolerant peptide serologies. Thus, our system allows rational large-scale serological assays with built-in quality control.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Hälsa och välfärd
- Health and Welfare
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Blomberg, Jonas
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Blomberg, Fredri ...
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Sjösten, Anna
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Sheikholvaezin, ...
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Bolin-Wiener, Ag ...
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Elfaitouri, Amal
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visa fler...
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Hessel, Sanna
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Gottfries, Carl- ...
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Zachrisson, Olof
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Öhrmalm, Christi ...
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Jobs, Magnus
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Pipkorn, Ruedige ...
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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Clinical and Vac ...
- Av lärosätet
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Högskolan Dalarna
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Uppsala universitet