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An exploration of the methods to determine the protein-specific synthesis and breakdown rates in vivo in humans.

Holm, Lars (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
Dideriksen, Kasper (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
Nielsen, Rie H (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
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Doessing, Simon (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
Bechshoeft, Rasmus L (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
Højfeldt, Grith (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
Moberg, Marcus, 1986- (author)
Gymnastik- och idrottshögskolan,Eva Blomstrands forskningsgrupp
Blomstrand, Eva (author)
Gymnastik- och idrottshögskolan,Eva Blomstrands forskningsgrupp,Karolinska institutet
Reitelseder, Søren (author)
Institute of Sports Medicine and Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark
van Hall, Gerrit (author)
University of Copenhagen, Denmark
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 (creator_code:org_t)
2019-09-08
2019
English.
In: Physiological Reports. - : John Wiley & Sons. - 2051-817X. ; 7:17
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The present study explores the methods to determine human in vivo protein-specific myofibrillar and collagenous connective tissue protein fractional synthesis and breakdown rates. We found that in human myofibrillar proteins, the protein-bound tracer disappearance method to determine the protein fractional breakdown rate (FBR) (via 2 H2 O ingestion, endogenous labeling of 2 H-alanine that is incorporated into proteins, and FBR quantified by its disappearance from these proteins) has a comparable intrasubject reproducibility (range: 0.09-53.5%) as the established direct-essential amino acid, here L-ring-13 C6 -phenylalanine, incorporation method to determine the muscle protein fractional synthesis rate (FSR) (range: 2.8-56.2%). Further, the determination of the protein breakdown in a protein structure with complex post-translational processing and maturation, exemplified by human tendon tissue, was not achieved in this experimentation, but more investigation is encouraged to reveal the possibility. Finally, we found that muscle protein FBR measured with an essential amino acid tracer prelabeling is inappropriate presumably because of significant and prolonged intracellular recycling, which also may become a significant limitation for determination of the myofibrillar FSR when repeated infusion trials are completed in the same participants.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Keyword

Amino acid recycling
deuterated alanine
deuterated water
fractional breakdown rate
fractional synthesis rate
protein turnover
stable isotope
Medicin/Teknik
Medicine/Technology

Publication and Content Type

ref (subject category)
art (subject category)

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