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The effects of pantothenate deficiency and acetate addition on anaerobic batch fermentation of glucose by Saccharomyces cerevisiae

Taherzadeh, Mohammad J, 1965- (author)
Chalmers University
Lidén, G (author)
Gustafsson, L (author)
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Niklasson, C (author)
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 (creator_code:org_t)
Springer, 1996
1996
English.
In: Applied Microbiology and Biotechnology. - : Springer. - 0175-7598 .- 1432-0614. ; 46:2, s. 176-182
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Physiological effects of deficiency of pantothenate, a necessary precursor in the synthesis of coenzyme A, were studied using the yeast strain Saccharomyces cerevisiae CBS 8066. Cells were grown on defined media in anaerobic batch cultures with glucose (50 g/l) as the carbon and energy source. Batch cultures containing more than 60 μg/l pantothenate showed no significant differences with respect to growth rates and product yields. However, with an initial pantothenate concentration of 30 μg/l, the average glucose consumption rate was 50% lower than in rich medium and, at even lower concentrations of pantothenate, the culture did not consume all the glucose in the medium. Furthermore, pantothenate deficiency caused the acetate and pyruvate yields to increase and the biomass yield to decrease, compared to the yields in pantothenate-rich medium. The increased acetate formation could be counteracted by initial addition of acetate to the medium, and thereby the glycerol yield could be decreased. An initial addition of acetate of 1.6 g/l to pantothenate-deficient medium (30 μg/l) caused a 35% decrease in glycerol yield and a 6% increase in ethanol yield. Furthermore, the time required for complete conversion of the glucose decreased by 40%. Acetate addition affected the acetate and glycerol yields in a similar way in pantothenate-rich medium (1000 μg/l) also.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)

Keyword

acetic acid
coenzyme a
glucose
pantothenic acid
anaerobic metabolism
article
biosynthesis
biotransformation
controlled study
fermentation
nonhuman
saccharomyces cerevisiae
Acetates
Culture Media
Fermentation
Glucose
Pantothenic Acid
Saccharomyces cerevisiae
Saccharomyces cerevisiae

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ref (subject category)
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Taherzadeh, Moha ...
Lidén, G
Gustafsson, L
Niklasson, C
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ENGINEERING AND TECHNOLOGY
ENGINEERING AND ...
and Industrial Biote ...
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University of Borås

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