Extraction and characterization of fungal chitin nanofibers from Mucor indicus cultured in optimized medium conditions

• Chitin nanofibers (ChNFs) were extracted from Mucor indicus by a purification method followed by a mechanical treatment, cultivated under obtained optimum culture medium conditions to improve fungal chitin production rate. A semi synthetic media containing 50 g/l glucose was used for cultivation of the fungus in shake flasks. The cell wall analysis showed that N-acetyl glucosamine (GlcNAc) content, which is an indication of chitin content, was maximum in presence of 2.5 g/l H3PO4, 2.5 g/l of NaOH, 1 g/l of yeast extract, 1 mg/l of plant hormones, and 1 ml/l of trace metals. The chemical characterizations demonstrated that the isolated fibers had a degree of deacetylation lower than of 10%, and were phosphate free. The FTIR results revealed successful removal of different materials during the purification step. The FE-SEM of fibrillated chitin illustrated an average diameter of 28 nm. Finally, X-ray diffraction results showed that the crystallinity index of nanofibers was 82%.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Biomaterial (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Bio Materials (hsv//eng)

Keyword

Chitin nanofiber

Mucor indicus

Optimized cultivation

chitin nanoparticle

glucose

n acetylglucosamine

phosphate

chitin

chitosan

fungal polysaccharide

nanofiber

Article

concentration (parameter)

controlled study

deacetylation

decolorization

field emission scanning electron microscopy

Fourier transform infrared spectroscopy

fungal biomass

fungal cell wall

high performance liquid chromatography

Mucor

nonhuman

purification

reaction optimization

scanning electron microscopy

X ray diffraction

analysis

biomass

chemistry

conditioned medium

culture medium

fractionation

infrared spectroscopy

isolation and purification

procedures

Chemical Fractionation

Culture Media

Culture Media

Conditioned

Fungal Polysaccharides

Nanofibers

Phosphates

Spectroscopy

Fourier Transform Infrared

X-Ray Diffraction

Resource Recovery

Resursåtervinning

Publication and Content Type

ref (subject category)

art (subject category)