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Platelets derived c...
Platelets derived citrullinated proteins and microparticles are potential autoantibodies ACPA targets in RA patients
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- Xu, Minjie (författare)
- Central China Normal University, Wuhan, China
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- Du, Rong (författare)
- Huazhong University of Science and Technology, Wuhan, China
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- Xing, Wenping (författare)
- Central China Normal University, Wuhan, China
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- Chen, Xueting (författare)
- Central China Normal University, Wuhan, China
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- Wan, Jian (författare)
- Central China Normal University, Wuhan, China
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- Xiong, Li (författare)
- Central China Normal University, Wuhan, China
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- Wang, Shengqiang (författare)
- Hospital affiliated to Central China Normal University, Wuhan, China
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- Nandakumar, Kutty Selva, 1965- (författare)
- Högskolan i Halmstad,Akademin för företagande, innovation och hållbarhet
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- Holmdahl, Rikard (författare)
- Karolinska Institutet
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- Geng, Hui (författare)
- Central China Normal University, Wuhan, China
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(creator_code:org_t)
- 2023-01-24
- 2023
- Engelska.
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Ingår i: Frontiers in Immunology. - Lausanne : Frontiers Media S.A.. - 1664-3224. ; 14
- Relaterad länk:
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https://doi.org/10.3...
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https://hh.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Citrullinated neoepitopes have emerged as key triggers of autoantibodies anti-citrullinated protein antibodies (ACPA) synthesis in rheumatoid arthritis (RA) patients. Apart from their critical role in homeostasis and thrombosis, platelets have a significant contribution to inflammation as well. Although anuclear in nature, platelets have an intricate post-translational modification machinery. Till now, citrullination in platelets and its contribution to trigger autoantibodies ACPA production in RA is an unexplored research direction. Herein, we investigated the expression of peptidylarginine deiminase (PAD) enzymes and citrullinated proteins/peptides in the human platelets and platelet derived microparticles (PDP). Both PAD4 mRNA and protein, but not the other PAD isoforms, are detectable in the human platelets. With a strict filtering criterion,108 citrullination sites present on 76 proteins were identified in the human platelets, and 55 citrullinated modifications present on 37 different proteins were detected in the PDPs. Among them, some are well-known citrullinated autoantigens associated with RA. Citrullinated forms of thrombospondin-1, β-actin, and platelet factor-4 (also known as CXCL4) are highly immunogenic and bound by autoantibodies ACPA. Furthermore, ACPA from RA sera and synovial fluids recognized citrullinated proteins from platelets and significantly activated them as evidenced by P-selectin upregulation and sCD40 L secretion. These results clearly demonstrate the presence of citrullinated autoantigens in platelets and PDPs, thus could serve as potential targets of ACPA in RA. Copyright © 2023 Xu, Du, Xing, Chen, Wan, Wang, Xiong, Nandakumar, Holmdahl and Geng.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- rheumatoid arthritis
- citrullination
- platelets
- anti-citrullinated protein antibodies (ACPA)
- platelet derived microparticles (PDP)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Xu, Minjie
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Du, Rong
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Xing, Wenping
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Chen, Xueting
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Wan, Jian
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Xiong, Li
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Wang, Shengqiang
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Nandakumar, Kutt ...
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Holmdahl, Rikard
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Geng, Hui
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Reumatologi och ...
- Artiklar i publikationen
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Frontiers in Imm ...
- Av lärosätet
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Högskolan i Halmstad
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Karolinska Institutet