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Considerations on C...
Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System
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- Chang, Keke (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Chen, Ruipeng (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Wang, Shun (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Li, Jianwei (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Hu, Xinran (author)
- School of Human Nutrition and Dietetics, McGill University, Canada.
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Liang, Hao (author)
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- Cao, Baiqiong (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Sun, Xiaohui (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Ma, Liuzheng (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Zhu, Juanhua (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China
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- Jiang, Min (author)
- College of life sciences, Henan Agricultural University, Zhengzhou, China
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- Hu, Jiandong (author)
- Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China; State key laboratory of wheat and maize crop science, Zhengzhou, China
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Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China School of Human Nutrition and Dietetics, McGill University, Canada (creator_code:org_t)
- 2015-08-19
- 2015
- English.
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In: Sensors. - : MDPI AG. - 1424-8220. ; 15:8, s. 20511-20523
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https://doi.org/10.3...
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https://www.mdpi.com...
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https://urn.kb.se/re...
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Abstract
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- The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 10(5) ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10(-5) refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- ADC conversion time
- biosensing system
- linear CCD array
- microcontroller
- refractive index
- surface plasmon resonance
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Chang, Keke
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Chen, Ruipeng
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Wang, Shun
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Li, Jianwei
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Hu, Xinran
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Liang, Hao
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show more...
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Cao, Baiqiong
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Sun, Xiaohui
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Ma, Liuzheng
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Zhu, Juanhua
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Jiang, Min
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Hu, Jiandong
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Sensors
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University of Gävle