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- Lindberg, A. MichaelHögskolan i Kalmar,Naturvetenskapliga institutionen (author)
Echovirus 5 : infectious transcripts and complete nucleotide sequence from uncloned cDNA
- Article/chapterEnglish1999
Publisher, publication year, extent ...
- 1999
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:hik-649
- https://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-649URI
- https://doi.org/10.1016/S0168-1702(98)00127-0DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Echovirus 5 (EV5) may be isolated from various neurological and exanthematic diseases. To determine the relationship of EV5 to other enteroviruses and for studies of its interactions with the target cell, the complete nucleotide sequence of EV5 was determined. Three overlapping fragments, collectively representing the complete genome, were amplified with RT–PCR and sequenced. Analysis of the EV5 sequence revealed a typical enterovirus-like organization of the genome. To verify that the cDNA generated sequence was derived from infectious viruses, complete EV5 genomes were amplified in one amplicon by long distance PCR. Transfection of in vitro transcribed RNA from these amplicons into cell cultures resulted in replicating EV5. Comparison of the overall nucleotide and amino acid sequences demonstrates that EV5 can be regarded as a coxsackievirus B-like enterovirus. Variable sequences between EV5 and the well characterized coxsackievirus B3 (CVB3) are for the most part observed for amino acid residues that correspond to exposed sequences in the CVB3 capsid. This observation indicates that the reported EV5 strain recently diverged from group B coxsackieviruses.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- Picornavirus
- Enterovirus
- Sequence analysis
- EV5; PCR
- 5′-RACE
- 3′-RACE
- In vitro transcription
- Transfection
- Microbiology
- Mikrobiologi
- Virology
- Virologi
- Virologi
- Virology
Added entries (persons, corporate bodies, meetings, titles ...)
- Johansson, SusanneHögskolan i Kalmar,Naturvetenskapliga institutionen (author)
- Andersson, AnneHögskolan i Kalmar,Naturvetenskapliga institutionen (author)
- Högskolan i KalmarNaturvetenskapliga institutionen (creator_code:org_t)
Related titles
- In:Virus Research59:1, s. 75-870168-1702
Internet link
- http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T32-3VKT49W-7-1&_cdi=4934&_user=745831&_orig=search&_coverDate=01%2F31%2F1999&_sk=999409998&view=c&wchp=dGLbVlb-zSkzk&md5=9548d37ca6b2107785043ded1c6cea24&ie=/sdarticle.pdfLänk till fulltext
- https://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-649
- https://doi.org/10.1016/S0168-1702(98)00127-0
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