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Human iPS-Derived A...
Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models
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- Lundin, Anders (author)
- Karolinska Institutet
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- Delsing, Louise (author)
- Gothenburg University,Göteborgs universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden / Institute of Neuroscience and Physiology, Department of Neurochemistry, the Sahlgrenska Academy at the University of Gothenburg, Mölndal, Sweden,Bioinformatik, Bioinformatics,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Clausen, Maryam (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Ricchiuto, Piero (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Sanchez, José (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Sabirsh, Alan (author)
- Pharmaceutical Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Ding, Mei (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Synnergren, Jane (author)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatik, Bioinformatics
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Brolén, Gabriella (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Hicks, Ryan (author)
- Discovery Sciences, IMED Biotech Unit, AstraZeneca, Mölndal, Sweden
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- Herland, Anna (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik,Department of Micro and Nanosystems KTH Royal Institute of Technology, Stockholm, Sweden / Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
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- Falk, Anna (author)
- Karolinska Institutet
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(creator_code:org_t)
- Cell Press, 2018
- 2018
- English.
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In: Stem Cell Reports. - : Cell Press. - 2213-6711. ; 10:3, s. 1030-1045
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Abstract
Subject headings
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- In vivo studies of human brain cellular function face challenging ethical and practical difficulties. Animal models are typically used but display distinct cellular differences. One specific example is astrocytes, recently recognized for contribution to neurological diseases and a link to the genetic risk factor apolipoprotein E (APOE). Current astrocytic in vitro models are questioned for lack of biological characterization. Here, we report human induced pluripotent stem cell (hiPSC)-derived astroglia (NES-Astro) developed under defined conditions through long-term neuroepithelial-like stem (ltNES) cells. We characterized NES-Astro and astrocytic models from primary sources, astrocytoma (CCF-STTG1), and hiPSCs through transcriptomics, proteomics, glutamate uptake, inflammatory competence, calcium signaling response, and APOE secretion. Finally, we assess modulation of astrocyte biology using APOE-annotated compounds, confirming hits of the cholesterol biosynthesis pathway in adult and hiPSC-derived astrocytes. Our data show large diversity among astrocytic models and emphasize a cellular context when studying astrocyte biology.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- apolipoproteins E
- astrocytes
- calcium signaling
- cell differentiation
- drug discovery
- glutamate plasma membrane transport proteins
- high-throughput screening assays
- induced pluripotent stem cells
- neurodegenerative diseases
- neuroinflammation
- Bioinformatik
- Bioinformatics
- INF502 Biomarkers
- INF502 Biomarkörer
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Lundin, Anders
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Delsing, Louise
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Clausen, Maryam
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Ricchiuto, Piero
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Sanchez, José
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Sabirsh, Alan
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show more...
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Ding, Mei
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Synnergren, Jane
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Zetterberg, Henr ...
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Brolén, Gabriell ...
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Hicks, Ryan
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Herland, Anna
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Falk, Anna
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Stem Cell Report ...
- By the university
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University of Skövde
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Royal Institute of Technology
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Karolinska Institutet
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University of Gothenburg